正常老年人EC301数字加工和计算量表检测研究

目的 研究正常老年人的年龄、不同教育程度、性别等因素对EC301-CR量表结果的影响.方法 对86例55～86岁老年人进行EC301-CR检测,获取计算能力数据材料,统计分析量表各亚项评分均数、标准差,分析年龄、性别、教育程度对评分的影响.结果 老年人教育程度影响其EC301-CR多数亚项检测评分,而年龄、性别的影响没有显著意义.结论 教育程度影响老年人计算能力,EC301-CR评分结果受教育程度的影响.     

动脉粥样硬化形成和消退的形态学研究

本文报告动脉粥样硬化(AS)形成和消退研究的部分工作。在复制AS形成和消退模型的基础上,研究了AS形成和消退中的内皮细胞、平滑肌细胞和单核细胞的改变。     

Lactate dehydrogenase content related to cross-sectional area in muscle fibres.

Cross-sectional areas and lactate dehydrogenase activity were determined of separate muscle fibres of mouse rectus femoris muscle in both ventral and dorsal parts of the muscle. The lactate dehydrogenase activity was measured quantitatively by microscope photometry using a tetrazolium staining in absence or presence of either vitamin K3 or phenazine methosulfate. If the observations are restricted to a relatively small area of the muscle cross section, one can distinguish categorically narrow ("red") and broad ("white) fibres with average values for cross sectional area and LDH content that depend on the part of the muscle chosen (higher LDH and smaller area in ventral part). However, if these data from the different parts or from the whole muscle are added together, no categorical distinction between the fibre types is allowed. On the basis of the observations a categorical division of muscle fibres within relatively large muscle areas or in a whole muscle is rather dubious. A relation is suggested between the subjective impression of different muscle fibre types in a field of observation with the number of motorunits present in that field.     

抗人DR5单抗-阿霉素交联物的制备及其细胞毒作用

目的：介绍抗人DR5单抗YM366EC-阿霉素结合物的制备及其细胞毒作用,以探讨YM366EC作为内源性导向载体的可能性。方法：采用氧化葡聚糖（Dex）T-40作为中介载体,联结抗人DR5YM366EC与阿霉素（ADR）制备交联物366EC-Dex-ADR,交联物中ADR与366EC的克分子比为71：1。经ELISA测定交联物的抗体活性大部分保持。MTT法体外测定其细胞毒性。结果：交联物对表达DR5受体的肿瘤细胞处理24小时的IC50是游离ADM的5倍,并且和肿瘤细胞DR5受体表达相关。结论：YM366EC具有良好的导向作用,结合物对肿瘤细胞有选择性杀伤作用。     

5—氨基水杨酸与乙基纤维素溶混性的计算机模拟

使用计算机辅助分子建模（ＣＡＭＭ）、分子力学和蒙特卡罗方法,模拟了５－氨基水杨酸（５－ＡＳＡ,Ｃ７Ｈ７ＮＯ３,美沙拉灵（ｓｐ－５７－６））与乙基纤维素（ＥＣ）的溶混性。模拟结果显示,在２７３Ｋ至３１６Ｋ的温度范围内,５－氨基水杨酸与乙基纤维素能以任何比例混溶。得出Ｅｍｉｘ（Ｔ）＝Ａ＋ＢＴ＋Ｃ／Ｔ模型,Ａ＝０．３６９４Ｅ＋０４,Ｂ＝－０．２６１０Ｅ－０２,ａｎｄＣ＝０．１８１８Ｅ＋０４,以及Ｅｍｉｘ（Ｔ）模型标准方差,Ｓ＝０．２６５４Ｅ－０３ｋｃａｌ／ｍｏｌ。对乙基纤维素及５－氨基水杨酸与乙基纤维素混溶过程的能量和构象分析表明,混溶过程中有乙基纤维素分子内和乙基纤维素与乙基纤维素分子间氢键形成。对两相混溶体系进行了热力学分析。     

Retrograde intubation dacryocystorhinostomy for proximal and midcanalicular obstruction

Objective

Retrograde intubation of canaliculi during dacryocystorhinostomy can restore canalicular patency in cases otherwise managed with bypass tubes. The surgical technique and success for this procedure are discussed.

Effect of low-dose phenobarbital on hepatic microsomal UDP-glucuronyl transferase activity

To determine whether hepatic microsomal enzyme induction occurs in rats following administration of phenobarbital at doses similar to those used in humans (0.5 to 7.5 mg/kg), UDP-glucuronyl transferase (UDPGT) and cytochrome P-450 activities were measured in liver homogenate and microsomal preparations from control rats and rats treated for 6 days with phenobarbital at 1 and 3 mg per kg per day. While no significant increases in liver weight and protein content of homogenate and microsomal preparations were observed with either dose of the drug, both UDPGT and P-450 activities were enhanced significantly following administration of phenobarbital at 3 mg per kg per day. The activity of P-450 was increased by approximately 30% and that of UDPGT by 15-24 and 45-66%, respectively, employing bilirubin and p-nitrophenol as the acceptor substrate. The extent of induction of bilirubin or p-nitrophenol UDPGT was similar when measured with "native" enzyme or with enzyme activated by UDP-N-acetyl glucosamine, digitonin or deoxycholate. These data suggest that the discordant effects of phenobarbital on UDPGT and cytochrome P-450 previously reported in humans and rats may not be attributable solely to differences in the drug doses employed.     

The immobilization response in rhesus monkey: a behavioral and electroencephalographic study.

Rhesus monkeys maintained in individual cages are rarely inactive when observed by humans unfamiliar to them. It has been observed that these animals display a greatly reduced behavioral repertoire after they are transferred to primate chairs. The present study used systemic behavioral observations to document those changes and to examine additional changes produced by arm restraint. Chair restraint was associated with a reduction in activity which was intensified when animals were further immobilized by arm restraint. This immobilization produced a reduction of tone in all limbs, a reduction of spontaneous behavior, and the appearance of eye closure. Electroencephalographic (EEG) correlates of the behavioral changes were examined also, using quantitative data generated through power spectral analysis of sensorimotor cortical EEG signals. Immobilization was accompanied by a significant increase in spectral density at 12 to 15 Hz which was most marked at mid and far lateral rholandic recording sites. No other significant changes were seen in the frequency bands studied. When the immobilized animal was alerted with novel stimuli, lower frequencies were attenuated but 12- to 15-Hz activity remained enhanced. These findings indicate that a unique immobilization response is elicited by restraint in the rhesus monkey which is associated with discrete changes in both behavior and accompanying EEG patterns.