酸性染料比色法测定甲氧异腈冻干品含量

本文采用酸性洒料比色法测定甲氧异腈冻干品的含量。结果表明，使用溴甲酚紫１．５ｍｌ，ＰＨ值为６．０的磷酸盐缓冲液５ｍｌ，氯仿５ｍｌ提取，经振摇２ｍｉｎ，静置１５ｍｉｎ为较理想比色条件，显色液稳定，甲氧异腈浓度在４－２０μｇ／ｍｌ范围内与吸收度呈良好线性关系。     

New high yield Cu(I) assisted 123I radioiodination of 15(p-I-phenyl)-9-methyl pentadecanoic acid,a potential myocardial tracer

A new high yield ¹²³I radioiodination (97%), based on the Cu(I) assisted isotopic exchange in an ethanol water mixture, of 15(p-I-phenyl)-9 methyl pentadecanoic acid, a potential myocardial tracer, is proposed. The method allows a true kit preparation of radioiodinated phenyl fatty acids for a substrate concentration of ±10^-6 moles. High specific activities (>0.1 mCi/g) can be obtained when coupling the labelling method to HPLC purification with an overall radiochemical yield of 75%.     

我国正常生育力男子精浆的微量元素研究

本文报告测试了68例正常生育力成年男子精浆中的锌、铜,铁等微量元素和镁,测试结果如下:锌130±5.64μg/ml,铜1.84±0.158μg/ml,铁0.801±0.104μg/ml,镁103.86±10.01μg/ml。所测得的数值均在正常范围内。在正常范围内的精液,精浆中的锌、镁含量与精液质量(精子密度和活动力)之间无显著差异。但铜的含量与精子活动度关系密切,含量高则活动度差,反之则活动度好。铁的含量与精子密度关系也十分密切,含量高时精子密度也高。     

Effect of maternal cadmium exposure on postnatal development and tissue cadmium,copper and zinc concentrations in rats

Administration of 60 ppm cadmium (Cd) in drinking water from the 1^st to the 20^th day of gestation to female rats did not affect the viability, body weight gain, food, and water consumption of offspring. The blood hemoglobin level was reduced in 2-week-old females and males but not in 16-week-old offspring. Hematocrit and serum glucose level were not affected at either age. Cadmium concentration in the intestinal wall was increased in both age groups, with marginal uptake in other organs. A decrease in copper (Cu) concentration was found in the brain of 2-week-old offspring of both sexes and of 16-week-old females. The brain zinc (Zn) concentration was decreased only in 16-week-old animals. The physical and neuromuscular development of offspring before weaning was not impaired by maternal Cd treatment. The alterations in Cu and Zn metabolism were associated with reduced locomotor activity and affected open-field behavior in adult offspring of either sex and with decreased avoidance acquisition in adult female offspring.The results obtained suggest a relationship between the reduced brain Cu and Zn levels and CNS dysfunction in adult offspring of female rats exposed to Cd during gestation.     

中国林蛙卵油和卵渣中微量元素的测定

目的：为中国林蛙油和卵渣应用提供科学依据。方法：采用等离子光谱和分光光度计法测定中国林蛙卵油和卵渣中的微量元素。结果：卵油和卵渣中均含 Ｚｎ, Ｍ ｎ, Ｃｕ, Ｆｅ 等 ９ 种人体必需微量元素,且 Ｚｎ／ Ｃｕ 比值较小。结论：卵油可能具有抗衰老及防治冠心病和高血压等作用。     

Effect of tetrakis-μ-3,5-diisopropylsalicylatodiaquodicopper(II) on the status of reduced glutathione in freshly isolated hepatocytes

Effects of different concentrations of tetrakis--3,5-diisopropylsalicylatodiaquodicopper(II) (Cu(II)₂(3,5-DIPS)₄(H₂O)₂) on the reduced status of glutathione (GSH), the major nonprotein thiol in tissues, were investigated using freshly isolated hepatocytes. Cu(II)₂(3,5-DIPS)₄ below 100 M did not have any significant effects on either the GSH content or viability of the hepatocytes, but at 150–250 M it decreased both parameters after 1 h of incubation. The decrease in cellular GSH was not followed by an increase in the oxidized form of GSH (GSSG) in the cell suspension. The addition of deferoxamine with Cu(II)₂(3,5-DIPS)₄ to the hepatocyte suspension prevented depletion in GSH content and loss of cell viability by Cu(II)₂(3,5-DIPS)₄. Both GSH depletion and loss of cell viability were found to be Cu(II)₂(3,5-DIPS)₄ dose dependent. From these results, it appears that Cu(II)₂(3,5-DIPS)₄ penetrated the cell membrane and acted by decreasing the GSH level by forming a copper-glutathione complex.     

罗氏促排卵汤加减对肾虚型无排卵性不孕症卵巢动脉血流动力学的影响

目的 观察“罗氏促排卵汤加减”对肾虚型无排卵性不孕症卵巢动脉血流动力学的影响，从“妇人以血为本”的角度探讨肾虚型无排卵性不孕症的发病机理和“罗氏促排卵汤加减”的作用机制。方法 用经阴道彩色多普勒超声测量30例正常妇女、30例肾虚偏肾阳虚型无排卵性不孕症患者治疗前后月经周期四时期最大卵泡平均直径(MFD)，卵巢动脉血流阻力指数(RI)和搏动指数(PI)。结果 健康组月经周期卵巢RI、PI有显著周期性变化。治疗组肾虚症状消失或缓解，卵泡增大，MFD与治疗前相比有显著差异，与健康组相比仍较低，仍有显著差异；RI值下降，与治疗前相比有非常显著差异，与健康组相比无显著差异，且有显著周期性改变；PI值下降，与治疗前相比有非常显著性差异，但与健康组相比仍有非常显著性差异，且无自身周期性变化。结论 “罗氏促排卵汤加减”作用机制是调整下丘脑-垂体-性腺轴，整体调节机体功能；对观察对象的影响是卵巢血管阻力降低，血供增加，血循环改善。     

铜、铁元素和麝香对癌细胞增殖与动力学影响的研究

目的：研究元素铜、铁、麝香对癌细胞增 殖及细胞周期的影响。方法：荷腹水肝癌 Ｂ Ａ Ｌ Ｂ／ｃ 小鼠分别ｉｇ 铜、铁、麝香制剂１２ 天,取腹水 癌细胞进行细胞分裂指数和流式细胞光度计分析。结果：铜、铁、麝香对癌细胞均有不同程度的抑制作用, 使分裂 指数 下降, 中小 细胞（ １０ ～２５μｍ ） 明 显增 加而 大 细胞（ ＞ ２５ μｍ ） 明 显 减少。铜、铁可使癌细胞主要阻滞在 Ｇ２ ＋ Ｍ 期。麝香对细胞大小和 细胞周期 基本无 影响。结论： 铜、铁使 癌细胞 主要阻滞在对抗癌药敏感的 Ｇ２ ＋ Ｍ 期。     

ICP-MS测定香芍软胶囊中砷 汞 铅 镉 铜的含量

目的：采用电感耦合等离子体质谱（ICP-MS）法测定香芍软胶囊（XSSC）囊心物中砷、汞、铅、镉、铜的含量。方法：样品经微波消解，以Re为内标，以茶叶、圆白菜标准物质为质控，采用ICP-MS测定上述5种元素。结果：对于所测5种元素，标准曲线的相关系数r〉0．9996，回收率为95．8-101．6％，RSD〈5．8％。结论：本测定方法快捷、准确、灵敏度高，适用于XSSC囊心物中上述5种元素的同时测定。