应用荧光免疫法检测生殖道沙眼衣原体及加德纳细菌

应用标记荧光素的抗沙眼衣原体(Chlamydia trachomatis,Ct)和抗阴道加德纳细菌(Gardnerella Vaginalis,GV)单克隆抗体,直接免疫荧光法(DFA)检测66例妇女生殖道标本。结果显示:Ct感染率为12.12％,GV感染率为19.70％。在妇科门诊患者Ct和GV感染率较高,应引起高度重视,18～40岁妇女是重点防治对象。     

泌尿生殖道患者沙眼衣原体和支原体检测及支原体药敏分析

目的　检测皮肤性病门诊泌尿生殖道患者沙眼衣原体 (CT)和支原体感染情况及支原体培养加药敏分析。方法　对5 0 95例泌尿生殖道感染者用金标法检测沙眼衣原体抗原 ,用微量肉汤稀释法检测解脲支原体 (Uu)和人型支原体 (Mh)并进行 8种抗生素的药敏试验。结果　 5 0 95例泌尿生殖道感染者中衣原体阳性 417例 ,总阳性率为 8 2 % ,女性患者阳性率 ( 11 2 % )明显高于男性患者阳性率 ( 6 2 % ) ,有显著性差异 ( χ2 =40 1,P <0 0 1) ;检出支原体 172 8例 ,总阳性率为 3 3 9% ,女性患者阳性率 ( 4 3 0 % )明显高于男性患者阳性率 ( 2 8 0 % ) ,有显著性差异 ( χ2 =114 8,P <0 0 1) ;其中Uu感染 ( 12 5 1例 ,2 4 6% )明显高于Mh感染 ( 71例 ,1 4% )和Uu +Mh混合感染 ( 4 0 6例 ,8 0 % )。Uu对红霉素、罗红霉素、交沙霉素、阿奇霉素、强力霉素、美满霉素、氧氟沙星、环丙沙星的高度敏感率分别为 60 3 %、67 5 %、73 2 %、85 3 %、5 5 7%、40 1%、2 5 6%、2 7%。Uu +Mh混合感染对 8种抗生素均出现不同程度的耐药菌株。结论　湖南地区泌尿生殖道感染患者中 ,衣原体、支原体感染处于国内较高水平。支原体对不同种类抗生素有较高的耐药性 ,特别是Uu +Mh混合感染 ;临床上做支原体培养加药敏试验 ,对指导     

免疫荧光法与 Giemsa 氏法检测沙眼衣原体的比较

本文用免疫荧光法与 Giemsa 氏法检测明显沙眼患者,两法阳性率相差无几。但在临床肉眼观察认为正常时,其阳性率分别为24.77%及11.58%(P<0.05),差异有显著意义,表明免疫荧光法检测早期沙眼较 Giemsa 氏法敏感。临床认为正常眼,经实验室检查25.56%为阳性,说明开展沙眼的实验室诊断是必要的。     

泌尿生殖道炎症患者沙眼衣原体感染的临床研究

目的　探讨在泌尿生殖道有炎症表现的情况下沙眼衣原体 (CT)感染的状况。方法　采用C -C快速法。结果　CT在泌尿生殖道炎症时的阳性率显著高于无炎症表现者 (P <0 .0 5) ;男性在有无炎症表现情况下的CT阳性率差异有显著性 (P <0 .0 5) ,而女性差异无显著性 (P >0 .0 5) ;2 1～ 50岁为高发年龄段。结论　CT感染症状隐匿 ,临床无症状者也应注意CT的检测 ,尤其是女性和高危人群。     

学报创刊15周年暨增刊通报

学报创刊１５周年暨增刊通报正值《中山医科大学学报》创刊１５周年之际，本刊编辑部于１９９５年６月１７日召开了“学报创刊１５周年总结会”。本刊编辑部主任、副主编关淡庄同志作了总结报告。首先感谢了学报的各位前辈为学报的创刊和发展所立下的汗马功劳，再次感谢了...     

不明原因不孕不育妇女子宫内膜沙眼衣原体检测

用ＰＣＲ检测３２例不明原因不孕、不育妇女子宫内膜沙眼衣原体，结果发现：２１例不明原因不孕妇女有７例阳性；８例不明原因自然流产２次以上妇女有３例阳性；３例不明原因死胎史妇女中１例阳性。结果揭示：子宫内膜的沙眼衣原体感染是引起不孕、不育的重要因素。     

微量沙眼衣原体组织培养法的建立及临床应用

建立了微量沙眼衣原体组织培养法，并应用于不同人群的生殖道标本的沙眼衣原体培养。结果生殖道沙眼衣原体培养的阳性率：男性尿道炎患者１４７％（９／６１）；正常孕妇３３％（７／２１０）；急性和亚急性盆腔炎女性患者６７％（７／１０４）；性病门诊患者１８２％（４９／２６９）。由于９６孔培养板及倒置荧光显微镜的应用，微量培养法试剂用量少、过程简化；自制的抗沙眼衣原体单克隆抗体大大降低了培养后的染色成本，适用于大宗标本。     

The occurrence of Chlamydia trachomatis in the semen of men participating in an IVF programme

Chlamydia trachomatis is one of the most common causative agentsof sexually transmitted diseases. The authors studied the occurrenceof C.trachomatis in the semen of 184 asymptomatic men participatingin the IVF programme. Twenty-six (14.1%) of the 184 tested werepositive for C.trachomatis, these patients and their wives receivingdoxycycline capsules twice, 100 mg on the first day and 100mg/day for the following 13 days. This treatment was effectivein 88.5% of the cases and in the rest, treatment continued witherithromycin 250 mg four times/day for 2 weeks. The authorscompared the semen parameters (cell count, motility, morphology,bovine mucus penetration and hypo-osmotic swelling test) inthe infected and non-infected groups and observed no significantdifference between these two groups     

Expression and Purification of the Major Outer Membrane Protein of Chlamydia Trachomatis in Prokaryotic Cell

Chlamydiatrachomatis(C.trachomatis)isanobligate intracellularbacterialpathogen.Ocularinfectionwith C.trachomatisserovarsA,B,BaandCleadstotracho ma,aleadingcauseofpreventableblindnessinmanyde velopingcountries[1].Urogenital tractinfectionwithC. trachomat…     

Value of papanicolaou smear in detection of Chlamydia trachomatis infection

This study was undertaken to assess the value of Papanicolaou smear for the diagnosis of Chlamydia trachomatis infection. The study was both retrospective (groups I and II) and prospective (group III). Group I consisted of 41 smears with cytomorphological changes proposed by Gupta, Kiviat, or Shiina. Group II was a control group, consisting of 30 cytologically normal smears. All these smears were subjected to specific immunofluorescent (IF) staining under identical conditions to confirm the diagnosis. In group III, 40 consecutive duplicate cervical smears were collected from patients attending the Sexually Transmitted Disease Clinic. One smear was routinely examined, and the specific IF staining was done on the other smear. The results in all the three groups were analysed. It was concluded that Papanicolaou smear is not useful in the detection of Chlamydia trachomatis infection.