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排序方式: 共有138条查询结果,搜索用时 15 毫秒
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【目的】研究栽培鸡血藤藤茎中次生代谢产物在不同生长期的动态积累变化趋势,为其采收期的合理制定提供科学依据。【方法】参照《中国药典》(2010年版一部)附录XA测定醇溶性浸出物的含量;采用紫外分光光度法测定总黄酮的含量,采用高效液相色谱法同时测定原儿茶酸、儿茶素及表儿茶素的含量。【结果】鸡血藤藤茎中醇溶性浸出物、总黄酮及指标性成分原儿茶酸、儿茶素和表儿茶素的含量均随着生长年限的增加呈递增的趋势;而1年中不同月份样品的醇溶性浸出物、总黄酮、表儿茶素的含量变化均呈抛物线状,7~9月含量达到峰值;儿茶素的含量则一直呈上升状态;7~9月采收的4~6年生鸡血藤样品中醇溶性浸出物已达到现行版《中国药典》的要求。【结论】初步建议栽培鸡血藤藤茎以7年生以上采收为宜,其最佳采收年限与采收月份有待进一步研究确定。 相似文献
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《Saudi Pharmaceutical Journal》2020,28(3):238-245
Polyphenols has attained pronounced attention due to their beneficial values of health and found to prevent several chronic diseases. Here, we elucidated binding mechanism between frequently consumed polyphenol “tea catechin” and milk protein bovine beta-lactoglobulin (β-Lg). We investigated the conformational changes of β-Lg due to interaction with catechin using spectroscopic and in silico studies. Fluorescence quenching data (Stern-Volmer quenching constant) revealed that β-Lg interacted with catechin via dynamic quenching. Thermodynamic data revealed that the interaction between β-Lg and catechin is endothermic and spontaneously interacted mainly through hydrophobic interactions. The UV-Vis absorption and far-UV circular dichroism (CD) spectroscopy exhibited that the tertiary as well as secondary structure of β-Lg distorted after interaction with catechin. Molecular docking and simulation studies also confirm that catechin binds at the central cavity of β-Lg with high affinity (~105 M−1) and hydrophobic interactions play significant role in the formation of a stable β-Lg-catechin complex. 相似文献
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Giovana Vechi Rita de Cássia Melo Vilhena de Andrade Fonseca da Silva Priscila de Souza Luísa Mota da Silva Sérgio Faloni de Andrade Valdir Cechinel Filho 《Pharmacological reports : PR》2019,71(5):950-957
BackgroundConsidering the therapeutic potential of phenolic compounds, the purpose of the present study was to investigate the mechanisms involved in the relaxation induced by cryptostrobin and catechin, isolated from Eugenia mattosii D. Legrand leaves, in the aorta of spontaneously hypertensive rats (SHR).MethodsThe thoracic aorta was isolated from SHR and kept in the organ bath system by recording contractile or relaxant responses.ResultsThe addition of cumulative concentrations of cryptostrobin and catechin induced endothelium-dependent and-independent relaxation in aorta rings from SHR, as well as both compounds were effective in reducing phenylephrine-induced contraction. Pretreatment of aortic rings with Nω-nitro-l-arginine methylester (L-NAME, an inhibitor of nitric oxide synthase) or 1H-[1,2,4] oxadiazolo[4,3-a]quinoxalin-1-one (ODQ, an inhibitor of soluble guanylate cyclase), resulted in a significant change of relaxant effect induced by catechin, and a slight influence on cryptostrobin-induced relaxation. Muscarinic receptor and potassium channels are involved in catechin-induced relaxation as assessed using atropine (a muscarinic receptor antagonist), tetraethylammonium (a non-selective K+ channel blocker) and glibenclamide (an ATP-sensitive K+ channel blocker). Conversely, cryptostrobin, but not catechin, blunted the contraction induced by the addition of phenylephrine in a calcium-free solution. Besides that, cryptostrobin attenuated the contraction of rat aorta rings induced by internal Ca2+ release and external Ca2+ influx.ConclusionsThese findings indicated that cryptostrobin and catechin alter vascular smooth muscle reactivity, and this effect may be involved, at least in part, by enhancing the endothelium NO/cGMP pathway and potassium channels activation. In addition, cryptostrobin reduced the phenylephrine, KCl and CaCl2-induced contractions in a calcium-free solution. 相似文献
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《Renal failure》2013,35(5):691-707
One of great use of immunosuppressant, Cyclosporine-A (CsA) is in the solid organ transplantation; however the extensive use of this is cautionable due to its toxic effect in renal tissue, characterized by the tubular atrophy, interstitial fibrosis, and progressive renal impairment. However, there are many mediators are associated with pathogenesis of nephrotoxicity of CsA, the exact mechanism is still in debate. Recent studies indicate that Reactive Oxygen Species (ROS) induced oxidative stress and lipid peroxidations are the important mechanisms implicated in the pathophysiology of nephrotoxicity with CsA. In the present study we examined effect of dietary flavonoid catechin on oxidative damage in cyclosporine-A induced nephrotoxicity. Chronic administration of CsA (20 mg/kg/day) subcutaneously for 21 days significantly decreased the body weight as compared with vehicle treated rats. CsA (20 mg/kg/day) administration for 21 days significantly decreased the renal function by increase in the serum creatinine, blood urea nitrogen, and decrease in the creatinine and urea clearance as compared with vehicle treated rats. Catechin (100 mg/kg/day) for 21 days along with CsA significantly reversed the changed renal parameters, however lower dose of catechin (50 mg/kg/day) restored only increased serum creatinine levels as compared with CsA alone treated group. Biochemical analysis revealed that chronic administration of CsA (20 mg/kg/day) for 21 days significantly induced lipid peroxidation and decreased the glutathione levels in the kidney homogenate of rats. It is also observed that chronic CsA administered rats showed decrease in antioxidant defense enzyme superoxide dismutase and increase in the catalase activity as compared with vehicle treated rats. Co-administration of catechin (100 mg/kg/day) orally along with CsA for 21 days significantly reduced the lipid peroxidation and restored the decreased glutathione levels as compared with CsA alone group, but lower dose of catechin (50 mg/kg/day) restored only decreased glutathione levels induced by CsA. Co-administration of only higher dose of catechin (100 mg/kg/day) along with CsA significantly increased the superoxide dismutase (SOD) levels as compared with CsA alone treated group. It is also observed that catechin (100 mg/kg/day) along with CsA further increased the catalase levels as compared with CsA alone treated group, but not with lower dose of catechin. Animals administered with catechin (100 mg/kg/day) alone for 21 days showed significant increase in the catalase levels as compared with vehicle treated group. The major findings of the present study suggest that oxidative stress might play a significant role in CsA-induced nephrotoxicity. In conclusion, dietary administration of flavonoid catechin could be a useful component for the prevention/treatment of CsA-induced nephrotoxicity. 相似文献
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目的:建立子宫锭中儿茶素的含量测定方法。方法:色谱柱为Agilent HC C18(250mm×4.6mm,5μm),流动相为0.04mol·L^-1枸橼酸溶液-N,N-二甲基甲酰胺-四氢呋喃(THF)(45:8:2),检测波长280nm,柱温35℃,流速1.0ml·min^-1。结果:儿茶素在501.2~1754.2ng之间呈良好的线性关系。平均加样回收率为98.47%,RSD=1.92%(n=9)。结论:方法简便可靠,分离度好,可用于子宫锭的质量控制。 相似文献
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儿茶药材中儿茶素和表儿茶素的高效液相色谱法分析研究 总被引:5,自引:0,他引:5
目的利用高效液相色谱法测定儿茶药材中儿茶素和表儿茶素的含量。方法色谱柱填料为十八烷基键合硅胶,流动相A:甲醇;流动相B:0.04 mol/L柠檬酸-N,N-甲基甲酰胺(45∶8,V/V)。A-B(15∶85,V/V),流速1m l/m in,柱温:35℃,检测波长280 nm。结果儿茶素在0.484~4.84μg、表儿茶素在0.184~1.84μg范围内呈良好的线性关系。两种成分的均回收率分别为98.11%和106.14%。结论该方法简便、准确、分离效果好,无干扰,可用于儿茶药材质量评价。 相似文献