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排序方式: 共有372条查询结果,搜索用时 15 毫秒
1.
Naruemon Klaikeaw Jutamas Wongphoom Duangporn Werawatganon Maneerat Chayanupatkul Prasong Siriviriyakul 《World journal of hepatology》2020,12(7):363-377
BACKGROUND Aloe vera exerts several biological activities, such as, anti-inflammatory, antioxidant, and antimicrobial effects. It was recently shown to reduce insulin resistance and triglyceride level. We hypothesized that aloe vera would have beneficial effects in alleviating non-alcoholic steatohepatitis(NASH) in rats.AIM To examine the therapeutic effects of aloe vera in NASH rats.METHODS All rats were randomly divided into 3 groups(n = 6 in each group). Rats in the control group were fed ad libitum with a standard diet for 8 wk. Rats in the NASH group were fed ad libitum with a high-fat high-fructose diet(HFHFD) for 8 wk. Rats in the aloe vera group were fed ad libitum with a HFHFD and aloe vera in dimethylsulfoxide(50 mg/kg) by gavage daily for 8 wk. Liver samples were collected at the end of the treatment period.RESULTS Hepatic malondialdehyde(MDA) levels increased significantly in the NASH group as compared with the control group(377 ± 77 nmol/mg vs 129 ± 51 nmol/mg protein, respectively, P 0.001). Glutathione(GSH) levels were significantly lower in the NASH group than the control group(9 ± 2 nmol/mg vs 24 ± 8 nmol/mg protein, respectively, P = 0.001). The expression of interleukin-18(IL-18), nuclear factor-kappa β, and caspase-3 increased, while peroxisome proliferator-activated receptor gamma decreased in the NASH group compared with the controls. Following aloe vera administration, MDA levels decreased(199 ± 35 nmol/mg protein) and GSH increased(18 ± 4 nmol/mg protein) markedly. Steatosis, hepatocyte ballooning, lobular inflammation and increased hepatocyte apoptosis were observed in the NASH group. Aloe vera treatment attenuated these changes in liver histology.CONCLUSION Aloe vera attenuated oxidative stress, hepatic inflammation and hepatocyte apoptosis, thus improving liver pathology in rats with NASH. 相似文献
2.
Susi Elaine Dal''Belo Lorena Rigo Gaspar Patrícia Maria Berardo Gonçalves Maia Campos 《Skin research and technology》2006,12(4):241-246
BACKGROUND/PURPOSE: The polysaccharide-rich composition of Aloe vera extracts (Aloe barbadensis Miller), often used in cosmetic formulations, may impart moisturizing properties to the product. The aim of this study was to evaluate the effect of cosmetic formulations containing different concentrations of freeze-dried Aloe vera extract on skin hydration, after a single and a 1- and 2-week period of application, by using skin bioengineering techniques. METHODS: Stable formulations containing 5% (w/w) of a trilaureth-4 phosphate-based blend were supplemented with 0.10%, 0.25% or 0.50% (w/w) of freeze-dried Aloe vera extract and applied to the volar forearm of 20 female subjects. Skin conditions in terms of the water content of the stratum corneum and of transepidermal water loss (TEWL) (Corneometer CM 825 and Tewameter TM 210) were analysed before and after a single and 1- and 2-week period of daily application. RESULTS: After a single application, only formulations supplemented with 0.25% and 0.50% (w/w) of Aloe vera extract increased the water content of the stratum corneum, while after the 2-week period application, all formulations containing the extract (0.10%, 0.25% and 0.50%) had the same effect, in both cases as compared with the vehicle. TEWL was not modified after a single and after 1- and 2-week period of application, when compared with the vehicle. CONCLUSION: Our results show that freeze-dried Aloe vera extract is a natural effective ingredient for improving skin hydration, possibly through a humectant mechanism. Consequently, it may be used in moisturizing cosmetic formulations and also as a complement in the treatment of dry skin. 相似文献
3.
目的 研究库拉索芦荟多糖对小鼠腹腔巨噬细胞的激活作用。方法 用25,50,100,200,400μg·mL-1的库拉索芦荟多糖体外诱导小鼠腹腔巨噬细胞48h ,测定细胞内乳酸脱氢酶、酸性磷酸酶、精氨酸酶活性及释放的TNF-α和IL-1含量。结果 库拉索芦荟多糖能上调腹腔巨噬细胞内乳酸脱氢酶、酸性磷酸酶和精氨酸酶活性,并能显著诱导腹腔巨噬细胞表达TNF-α和IL-1,增强吞噬中性红能力。结论 库拉索芦荟多糖对小鼠腹腔巨噬细胞具有激活作用。 相似文献
4.
目的: 优选库拉索芦荟多糖的醇沉工艺。方法: 以多糖得率为指标,选取浓缩程度、醇沉浓度、醇沉时间为考察因素,通过正交试验优选库拉索芦荟多糖的醇沉工艺。采用硫酸-苯酚法测定芦荟多糖含量。结果: 最佳醇沉工艺条件为新鲜库拉索芦荟多糖提取液浓缩比例1:2,醇沉体积分数70%,醇沉时间6 h;芦荟多糖平均得率达0.076%,平均纯度57.37%。结论: 该优选的工艺条件科学合理,可用于库拉索芦荟多糖的工业化生产。 相似文献
5.
反相高效液相色谱法测定芦荟中芦荟苷及芦荟大黄素 总被引:16,自引:0,他引:16
目的:建立以高效液相色谱法测定芦荟鲜汁及复方芦荟胶囊中的芦荟大黄素的方法。方法:A法以甲醇-水(45:55)为流动相,于354nm波长处以外标法直接测定样品中芦荟;B法先将样品加三氯化铁和盐酸水浴加热回流使芦荟苷水解为芦荟大黄素后在287nm波长处,以甲醇-水(70:30)为流动相,以外标法测定。结果:芦荟苷及芦荟大黄素线性范围均为0.04-0.4цg。在此范围内浓度与峰面积线性关系良好(r=0.9999)。芦荟苷的平均回收率为102.1%(复方芦荟胶囊),RSD为1.37%;芦荟大黄素平均回收率为102.5%(复方芦荟胶囊),RSD为3.6%。结论:该两种方法均比较灵敏、准确,而A法直接测定芦荟苷更为简便、快捷。 相似文献
6.
Extracts of Aloe vera whole plant, Eucalyptus globulus leaves, Ficus infectoria bark, Ficus religiosa bark and Piper betel leaves were studied for antibacterial activity on resistant and sensitive strains, isolated from skin and soft tissue infections. A combination of hot alcoholic extracts of Ficus infectoria, Ficus religiosa and Piper betel were found to be more effective against all the isolates. The combined extract was formulated in different ointment bases such as polyethylene glycol, gelatin, sodium alginate, carbopol, cream base and honey. These were then evaluated to find a suitable base for preparation of an ointment. In vitro study of the release of antimicrobials and kill-time studies of the herbal ointments was carried out against multi-drug resistant isolate of Pseudomonas. The ointment showed bactericidal activity within 2 h against the resistant strain of Pseudomonas spp. 相似文献
7.
目的研究库拉索芦荟多糖的保湿特性、经皮吸收特性以及皮肤安全性。方法采用超声辅助提取芦荟活性多糖,并通过人体皮肤水分含量和水分散失量测试法对所制备的芦荟活性多糖的保湿特性(包括锁水、保水两个方面)进行评价;Franz扩散池体外透皮吸收法评价其透皮吸收特性;人体斑贴试验评价其皮肤安全性。结果与空白对照组比较,库拉索芦荟多糖可显著提高皮肤水合状态(P〈0.05),同时降低经皮失水(P〈0.05);多糖质量浓度为5%时,皮肤渗透速率达到0.2510mg/(h·cm2),24h累计渗透量达(4.1519±0.0462)mg/cm2;人体皮肤斑贴试验中全部评定为0级反应。结论库拉索芦荟活性多糖对皮肤具有显著的保湿功效以及较强的皮肤渗透力,并且高度安全,可作为优良的保湿添加剂在化妆品等肤用产品中广泛应用。 相似文献
8.
目的:建立高效液相色谱法测定好望角芦荟中芦荟苷的含量。方法:流动相为乙腈-水(25:75),检测波长355nm,样品提取过滤后进行分析。结果:线性范围为0.4008~4.008μg·μL^-1,相关系数为r=0.9997,精密度为0.7%,加样回收率为99.7%。好望角芦荟中芦荟苷的含量为20.1%。结论:该法简便、灵敏,结果可靠准确,适用于芦荟中芦荟苷的含量测定。 相似文献
9.
芦荟抗菌作用与蒽醌化合物的关系 总被引:36,自引:1,他引:36
目的 :分析芦荟抗菌作用与蒽醌化合物含量的关系 ,比较芦荟大黄素和芦荟苷的抗菌作用 ,探讨糖基取代结构对芦荟苷抗菌活性的影响。方法 :利用琼脂扩散法分析皂质芦荟叶皮提取物、芦荟大黄素和芦荟苷对 3种革兰氏阴性菌、2种革兰氏阳性菌的抑菌活性 ,利用扫描电镜技术分析了芦荟苷对大肠杆菌的作用。结果 :芦荟的抗菌作用与蒽醌化合物的含量呈正相关性。芦荟苷 (1g·L-1)具有较强的抗菌活性 [抑菌直径大于 (7.1±0.15)mm] ,对革兰氏阳性菌的抗性高于革兰氏阴性菌。芦荟大黄素的抗菌活性较弱。芦荟苷能够改变细胞的形态 ,破坏细胞结构。结论 :蒽醌化合物是芦荟中的抗菌成分 ,芦荟苷是主要抗菌有效成分之一 ,葡萄糖基取代结构增强了其侵入细胞外被和杀菌的能力。 相似文献
10.
闽产木立芦荟多糖的分离纯化及初步分析 总被引:14,自引:2,他引:12
目的 分离纯化及初步分析产木立芦荟中的多糖。方法 芦荟经沸水提取,醇沉,Sevage法去除蛋白质,Sephadex G-200柱色谱分离纯化,制得闽产木立芦荟多糖,以硫酸-苯酚法测定其总糖含量。结果 闽产木立芦荟多糖经紫外吸收光谱扫描具有多糖特征吸收峰,未见核酸和蛋白质吸收峰,多糖含量可达89.7%,初步分析由D-甘露糖和D-葡萄糖等单糖组成。结论 从闽产木立芦荟中可提取分离纯度较高的杂多糖。 相似文献