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排序方式: 共有126条查询结果,搜索用时 31 毫秒
1.
Lin Luo Tingting Xu Pengbo Wang Liuliu Mao Chenghao Xi Jinhua Huang Wei Zhang 《Experimental and toxicologic pathology》2017,69(2):73-81
The pathological changes of parasympathetic nerve are considered as an independent prognostic factor of the survival rate of patients with chronic liver disease. The non-selective muscarinic acetylcholine receptors (mAchR) agonists and antagonists can affect the proliferation of hepatocytes and hepatic stellate cells, but the subtypes of mAchR expressions in HCs are still uncertain. Here, we investigate the expression of mAchR in hepatic fibrosis on rats. 3 ml/kg 40% carbon tetrachloride (CCL4) was given to induce hepatic fibrosis on rats and the hepatocytes were isolated. Compared to the normal state, the expression levels of m1, 3, 5 in fibrotic liver tissues or hepatocytes were obviously increased, while m2, 4 decreased. 10 μM pilocarpine or 10 μM acetylcholine could increase the alanine aminotransferase (ALT), hydroxyproline (Hyp), collagen I, III in the hepatocytes, and decreased albumin (ALB). They also changed the expressions of mAchR similarly as the fibrotic hepatocytes and livers. However, atropine could ameliorate the state of fibrotic hepatocytes. These data indicate that mAchR played an important role in the regulation of hepatic fibrosis process. Targeting mAchR would have therapeutic potential for hepatic fibrosis. 相似文献
2.
Bowen Qi Ayrianne J. Crawford Nicholas E. Wojtynek Megan B. Holmes Joshua J. Souchek Graca Almeida-Porada Quan P. Ly Samuel M. Cohen Michael A. Hollingsworth Aaron M. Mohs 《Nanomedicine : nanotechnology, biology, and medicine》2018,14(3):769-780
Pancreatic ductal adenocarcinoma is highly lethal and surgical resection is the only potential curative treatment for the disease. In this study, hyaluronic acid derived nanoparticles with physico-chemically entrapped indocyanine green, termed NanoICG, were utilized for intraoperative near infrared fluorescence detection of pancreatic cancer. NanoICG was not cytotoxic to healthy pancreatic epithelial cells and did not induce chemotaxis or phagocytosis, it accumulated significantly within the pancreas in an orthotopic pancreatic ductal adenocarcinoma model, and demonstrated contrast-enhancement for pancreatic lesions relative to non-diseased portions of the pancreas. Fluorescence microscopy showed higher fluorescence intensity in pancreatic lesions and splenic metastases due to NanoICG compared to ICG alone. The in vivo safety profile of NanoICG, including, biochemical, hematological, and pathological analysis of NanoICG-treated healthy mice, indicates negligible toxicity. These results suggest that NanoICG is a promising contrast agent for intraoperative detection of pancreatic tumors. 相似文献
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4.
D Schmitt N Tran S Riefler J Jacoby D Merkel P Marone N Naouli 《Food and chemical toxicology》2008,46(3):1048-1054
Modified gum acacia, produced from acacia gum by a process analogous to the production of modified food starch, was tested for mutagenicity in the microbial reverse mutation assay. The assay employed a wide range of dose levels, both with and without metabolic activation. Test results gave no indication that modified gum acacia possessed any mutagenic potential. The acute oral toxicity of modified gum acacia was determined in two studies employing Sprague-Dawley rats, and the LD50 values were found to be >2000 mg/kg. The primary dermal irritation potential of modified gum acacia was evaluated in rabbits by the Draize method. Test results indicated that modified gum acacia was slightly irritating by the Environmental Protection Agency (EPA) classification but not a primary irritant by Consumer Product Safety Commission (CPSC) guidelines. The subchronic toxicity of modified gum acacia was examined in Sprague-Dawley rats fed diets containing 0%, 1%, 2.5%, and 5% modified gum acacia for 13 weeks. No dose-related effects on survival, growth, hematology, blood chemistry, organ weights, or pathologic lesions were observed. Results of these studies indicate that modified gum acacia does not possess mutagenic potential and that animals are not adversely affected by acute or subchronic exposure to modified gum acacia. 相似文献
5.
目的探讨C反应蛋白/白蛋白(CRP/ALB)、淋巴细胞与单核细胞比值(LMR)对溃疡性结肠炎(UC)内镜下病情严重程度的评估价值。 方法收集189例UC患者,根据内镜下严重程度指数评分分为缓解组和活动组(包括轻度组、中度组和重度组),比较各组CRP/ALB、LMR和白细胞计数(WBC),采用ROC曲线分析CRP/ALB、LMR对UC内镜下病情严重程度的评估价值。 结果活动组患者WBC、CRP/ALB水平高于缓解组,LMR水平低于缓解组,且随内镜下病情严重程度增加趋势更明显(P<0.05)。ROC曲线分析显示,CRP/ALB、LMR单独及联合检测对UC内镜下病情严重程度均有预测价值,且联合检测的预测价值最高(P<0.05)。 结论LMR、CRP/ALB联合检测对UC内镜下病情严重程度有良好预测效能,可作为临床评估UC内镜下活动和重度UC的辅助指标。 相似文献
6.
Fukuda I Tsutsui M Yoshida T Toda T Tsuda T Ashida H 《Food and chemical toxicology》2011,49(12):3272-3278
Black soybean (Glycine max) has been used for traditional medicine and food in Asian countries, but safety of its hull has not been studied. We conducted acute and chronic oral toxicity studies. For the acute study, an extract of black soybean hull (BE; 2.5 g/kg body weight) was administered singly by intragastric intubation to Sprague–Dawley rats and C57BL/6 mice. There was no death or significant decrease in body weight in rats and mice, and the oral LD50 of BE was >2.5 g/kg body weight. In the chronic study, BE was administered at dietary levels of 0% (control), 2.0%, and 5.0% to male and female C57BL/6 mice for 26 weeks. No mortality or toxicologically significant clinical changes were observed through the experimental period. Although body weights, as well as abdominal fat, blood levels of triglyceride and total cholesterol in 5.0% males were significantly lower than that in control and 2.0% groups, these changes were considered not to be adverse. Hematology and histopathological observation revealed no toxicologically significant changes. The no-observed adverse-effect-level of BE was estimated to be 5.0% in the diet (5074.1 mg/kg body weight/day for males and 7617.9 mg/kg body weight/day for females). 相似文献
7.
早产儿及足月儿蛋白质水平差异的研究 总被引:1,自引:1,他引:0
目的 通过对早产儿及足月儿蛋白质指标的分析,找到更敏感更准确的反映新生儿营养的指标,并为早产儿营养支持提供依据.方法 依据入选标准对2008~2010年本院新生儿病房的部分早产儿及同期本院产科出生的部分足月适于胎龄儿分段抽血查前清蛋白(PA)、清蛋白(ALB)、总蛋白(TP),并行统计学分析.结果 胎龄越小或体质量越轻者,PA越低;早产儿在采用静脉营养后PA有显著升高;早产小于胎龄儿、早产适于胎龄儿、早产大于胎龄儿间也存在PA的差异.结论 PA作为营养指标较ALB、TP更敏感,可更准确地反映新生儿不同时期的营养状况,并为早产儿营养支持提供依据. 相似文献
8.
The toxicological effects of realgar after intragastrical administration (1 g/kg body weight) were investigated over a 21 day period in male Wistar rats using metabonomic analysis of 1H NMR spectra of urine, serum and liver tissue aqueous extracts. Liver and kidney histopathology examination and serum clinical chemistry analyses were also performed. 1H NMR spectra and pattern recognition analyses from realgar treated animals showed increased excretion of urinary Kreb's cycle intermediates, increased levels of ketone bodies in urine and serum, and decreased levels of hepatic glucose and glycogen, as well as hypoglycemia and hyperlipoidemia, suggesting the perturbation of energy metabolism. Elevated levels of choline containing metabolites and betaine in serum and liver tissue aqueous extracts and increased serum creatine indicated altered transmethylation. Decreased urinary levels of trimethylamine-N-oxide, phenylacetylglycine and hippurate suggested the effects on the gut microflora environment by realgar. Signs of impairment of amino acid metabolism were supported by increased hepatic glutamate levels, increased methionine and decreased alanine levels in serum, and hypertaurinuria. The observed increase in glutathione in liver tissue aqueous extracts could be a biomarker of realgar induced oxidative injury. Serum clinical chemistry analyses showed increased levels of lactate dehydrogenase, aspartate aminotransferase, and alkaline phosphatase as well as increased levels of blood urea nitrogen and creatinine, indicating slight liver and kidney injury. The time-dependent biochemical variations induced by realgar were achieved using pattern recognition methods. This work illustrated the high reliability of NMR-based metabonomic approach on the study of the biochemical effects induced by traditional Chinese medicine. 相似文献
9.
Silk-elastinlike protein polymers (SELPs) are recombinant polymers designed from silk fibroin and mammalian elastin amino acid repeats. These are versatile materials that have been examined as controlled release systems for intratumoral gene delivery. SELP hydrogels comprise monodisperse and tunable polymers that have the capability to control and localize the release and expression of plasmid DNA and viruses. This article reviews recent developments in the synthesis and characterization of SELP hydrogels and their use for matrix-mediated gene delivery. 相似文献
10.
Hedi Schelleman Colleen M Brensinger Jinbo Chen Brian S Finkelman Mark J Rieder Stephen E Kimmel 《British journal of clinical pharmacology》2010,70(3):393-399