Focal cerebral ischemia alters the spatio-temporal properties, but not the amount of locomotor activity in mice

Cerebral ischemia induces hyperlocomotion in gerbils, and sensorimotor and cognition dysfunctions in rodents ; however little is known about the spatio-temporal organization of locomotor activity after ischemia. In this study, we continuously （22 h） assessed the spatio-temporal organization of locomotor activity in focal cerebral ischemic mice in an open field with feeding and drinking supplies. To observe the spatio-temporal changes,     

Construction of pETNF-P16 plasmid and its expression properties in EC9706 cell line induced by X-ray irradiation

AIM: Recombined plasmid pETNF-P16 was constructed to investigate its expression properties in esophageal squamous carcinoma cell line EC9706 induced by X-ray irradiation and the feasibility of gene-radiotherapy for esophageal carcinoma. METHODS: Recombined plasmid pETNF-P16 was constructed and transfected into EC9706 cells with lipofectamine. ELISA,Western blot, and immunocytochemistry were performedto determine the expression properties of pETNF-P16 in EC9706 after transfection induced by X-ray irradiation. RESULTS: Eukaryotic expression vector pETNF-P16 was successfully constructed and transfected into EC9706 cells. TNFα expressions were significantly increased in the transfected cells after different doses of X-ray irradiation than in those after 0Gy irradiation (1 192.330-2 026.518 pg/mL,P<0.05-0.01), and the TNFα expressions and P16 were significantly higher 6-48 h after 2 Gy X-ray irradiation (358.963-585.571 pg/mL, P<0.05-0.001). No P16 expression was detected in normal EC9706 cells. However, there was strong expression in the transfected and irradiation groups. CONCLUSION: X-ray irradiation induction could significantly enhance TNFα and P16 expression in EC9706 cells transfected with pETNF-P16 plasmid. These results may provide important experimental data and therapeutic potential for gene-radiotherapy of esophageal carcinoma.     

药物心脏毒副作用及其产生机制与防治策略(上)

目的综述药物产生心脏毒副作用的及产生机制,介绍近年来各类药物产生继发性心脏毒副作用的临床表现及防治策略,为临床用药提供参考.方法阅读国内外相关的文献资料,进行整理、分析和综合评价.结果目前临床使用的药物中有数十种被报道具有心脏毒副反应,涵盖抗肿瘤药物、抗菌药物、抗疟药物、抗过敏药物以及局麻药等,结构差异大,心脏毒副作用的产生机制复杂.结论临床用药须了解各类药物产生心脏毒副作用的病理表现,密切注意患者各心脏指标的变化,在给患者带来预期疗效的同时也确保安全.     

膀胱女性的多事之地

从解剖学上来看,女性尿道具有短、宽、直和肌肉力量薄弱等特点,同时比较接近阴道和肛门,因此常会给膀胱增添许多麻烦.     

小厨房学习大能力

长久以来,厨房一直被视为幼儿的危险禁地。然而,对幼儿而言,厨房是—个充满创意与无限可能的魔法空间,各式各样的烹饪工具与食材,等着尽情去探索。任务完成后,享受美味的餐点作品与家人的赞美,更是孩子成长过程中最实质的鼓励与回馈。     

如意的前世与今生

<正>人们见面或通信时,往往会互祝对方万事如意,如意即如人之意。然而,如意原本是一种实物,一种用玉石或金属制成的供人收藏观赏的精美工艺品。     

影响性爱的“浪漫”道具

红酒、咖啡、鸳鸯浴,催情大餐……电影导演们的丰富想象力撩拨着平平凡凡的饮食男女们的情欲。正如不能将电影情节当真一样,其实那些所谓的浪漫道具并不一定能让你拥有一次激情的性爱,可能还会破坏你的性爱质量。     

健康小道具怎么选？

1体重秤 如果在女孩子中做一个调查，你们最关心什么？体重绝对是名列前三甲的答案！在这个以骨感为美的风潮中，左右女人心情的就是体重秤上指针的移动方向——向左走，还是向右走？如此重要的体重秤，你会挑选吗？     

好莱坞巨片《魔戒》三部曲制作解密

电影《魔戒》之三在美国的公映历经数月，最近终于徐徐落幕。2004年3月，在76届奥斯卡金像奖中囊括了11项大奖，总算是功德圆满。     

乐活MM24小时养肝道具秀

养肝是个系统工程,需要借助一些生活中的小道具,才可以在24小时内为肝脏调养解毒,确保它出色地完成工作。要想身体健康,必须先排毒;而提到排毒,必然说到肝脏这个辛苦的首席＂排毒师＂。养肝是个系统工程,需要借助一些生活中的小道具,才可以在24小时内为肝脏调养解毒,确保它出色地完成工作。