应用σ度量对深圳市龙华区三家综合医院临床生化检验结果互认的评价研究

目的 探讨σ度量在深圳市龙华区三家综合医院临床生化检验结果 互认中的应用价值.方法 收集三家医院2019年广东省临检中心常规化学,特殊蛋白前两次的室间质评(EQA)样本,经混匀并分割成比对样本送至各评价实验室进行检测;以卫生部临检中心(NCCL)标准和生物学变异导出的允许总误差(TEa％)为质量规范,通过加权法计算出各...     

应用六西格玛统计技术对A型肉毒毒素中间品量器的分析

目的应用六西格玛(6σ)统计技术对A型肉毒毒素中间品取用时所用的量出式量器进行分析研究。方法以偏倚-线性做考察指标研究对比分度吸管及移液器的准确度,对吸管进行变异源分析(单因素、双因素交叉及嵌套设计分析)。结果分度吸管未发生偏倚,较移液器具有更好的准确度;人员、操作批次对吸管量取效果无显著影响,吸管间的差异是主要影响因素。结论通过对测量得出的结果进行分析,确定吸管和三通吸球为最佳量器组合,同时吸管间的差异是影响其的主要因素。     

Gamma-glutamyl transferase: A sensitive marker in experimental hexachlorobenzene intoxication

The behaviour of gamma-glutamyl transferase (γ-GT) in rat liver, serum and intestinal mucosa under the effect of hexachlorobenzene (HCB) was studied. The investigation was carried out in order to evaluate γ-GT as a biological parameter in experimental HCB porphyria. HCB was administered by gavage at daily doses of 3.5 mmol/kg body wt for 60 days or 90 days.γ-GT in liver homogenate progressively increased during the treatment. Following a 60-day exposure the enzymatic activity exceeded the values of the controls by more than 60 times. The rise of serum γ-GT was more moderately expressed (5–6-fold).γ-GT in jejunal mucosa was elevated 2-fold after a 90-day treatment. The termination of the daily intoxication reduced back to normal values the enzymatic activity in liver and in intestine as measured after 60 days from the cessation of HCB application.It can be pointed out, that γ-GT is a sensitive and valuable marker for experimental HCB intoxication.     

六西格玛质量管理体系在医院药疗差错监控管理的应用

目的:优化药物治疗的各个环节,降低药疗差错率.方法:采用六西格码管理的循环改进法(即DMAIC模式),包括:定义、评估、分析、改进、控制5个步骤,分别找出关键点,分析造成药疗差错的主要因素,制定解决问题的措施,然后加以控制.结果:干预后处方差错及给药差错得到明显的降低,分别从0.524%、0.11%降低到0.397%与0.088%(P<0.05);总体的药疗差错事件得到显著的降低,从0.6643%降低到0.5062%.结论:六西格玛质量管理体系理论应用于药疗差错监控系统,有效降低我院的药疗差错率,收效明显.     

散发性乳腺癌细胞14-3-3σ异常甲基化及其蛋白表达的关系

目的:探讨散发性乳腺癌癌变过程14-3-3σ基因启动子异常甲基化状况及其与转录表达的关系。方法:用甲基化特异PCR方法对散发性乳腺癌病人癌组织及正常组织进行14-3-3σ异常甲基化检测;用免疫组织化学测定14-3-3σ蛋白表达。结果:68例散发性乳腺癌组织中,14-3-3σ基因启动子异常甲基化率为90%(61/68),部分不典型增生病例检出异常甲基化18%(2/13),而正常组织未检出。14-3-3σ基因启动子异常甲基化与患者居住地、组织分型、分级和淋巴结转移相关(P<0.05)而与年龄无相关性(P>0.05),14-3-3σ基因甲基化与其蛋白表达下降呈良好的一致性(P<0.05)。单变量分析发现,淋巴结转移与预后明显相关(风险指数为:5.0,P=0.02)。14-3-3σ基因甲基化与否并不影响乳腺癌的五年生存率(P>0.05)。结论:14-3-3σ基因异常甲基化参与乳腺癌癌变过程,是其蛋白表达下降的重要原因之一,但不影响乳腺癌的生存率。     

Optimum support properties for protein separations by high-performance size exclusion chromatography

Optimum chromatographic properties of high performance size exclusion chromatography (HPSEC) of proteins, such as resolution, molecular weight accuracy and recovery, are obtained on packings and columns with tailor-made physical and chemical structures, employed at properly adjusted eluent compositions and operation conditions. SEC-theory suggests that a broad molecular weight fractionation range and high linearity of the log-linear calibration plot can be achieved by the use of two packings (10- and 80-nm pore size, characterized by a pore-size distribution (psd) equal to or less than 1 decade and by equal internal column porosity (p)), rather than a single 30- to 50-nm pore-size packing with a wide psd. Favourably high-phase ratios of (p)/(o)/ 1.0 for HPSEC columns were accomplished with a minimum interstitial column porosity (o) and a high value for the internal column porosity (p) (the specific pore volume, nu(p), multiplied by the packing density, varrho(p).) Ligands such as diol, N-acetoxyamino and oligomeric ether with a propyl- or propoxy-spacer bonded to the silica at the highest density appear to provide high mass recovery and bioactivity as well as chemical stability. Such packings, available in 3-5 mum particle size ranges of narrow distribution, packed into columns 6 mm i.d. and 500 mm in length, offer the best compromise with respect to resolution, speed and pressure drop. More careful studies are required to explain the effects of protein conformational changes and interconversions during elution on HPSEC columns.     

14-3-3σ调控p27抑制Rat1-Akt细胞增殖

目的： 研究腺病毒介导14-3-3·σ（Ad-14-3-3·σ）对Akt过表达Rat1-Akt细胞增殖的影响，并探讨其作用是否通过调控p27而实现。 方法： 通过5-溴-2′脱氧尿嘧啶（BrdU）实验检测Ad-14-3-3·σ对Rat1-Akt细胞增殖的影响，并通过激酶分析法和免疫荧光实验探讨Ad-14-3-3·σ对p27磷酸化水平及其在细胞内定位的影响。 结果： Ad-14-3-3σ转染的细胞BrdU阳性率（45%）低于PBS处理组（100%）或Ad-β-gal转染的对照组细胞（98%）。14-3-3σ可降低磷酸化p27的水平和减少Akt介导的p27在胞浆中的定位。 结论： 转染14-3-3σ基因能抑制Akt过表达细胞株Rat1-Akt增殖，14-3-3σ通过降低Akt激酶磷酸化p27的活性，阻断Akt介导的p27胞浆错位，从而发挥其抑制Rat1-Akt细胞增殖。     

σ-1受体在糖尿病视网膜病变中神经保护作用的研究进展

视网膜微血管病变被认为是糖尿病视网膜病变(diabetic retinopathy,DR)的重要特征之一,近年来越来越多的研究表明,DR同时有视网膜神经元的损害.σ-1受体是一种能与多种抗精神病药物结合的非阿片类蛋白受体,近年来许多研究发现,σ-1受体的激活有视网膜神经保护作用.本文综述了σ-1受体在DR中神经细胞保护作用机制的研究进展,为DR神经损害的研究及防治提供新的思路.     

14-3-3σ基因影响人低分化鼻咽癌细胞CNE-2的信号通路

目的探讨14-3-3σ对鼻咽癌CNE-2细胞的信号通路的影响。方法通过反相蛋白质阵列检测14-3-3σ转染鼻咽癌CNE-2细胞前后特定蛋白质表达量的变化。结果10种蛋白的表达量在14-3-3σ转染CNE-2细胞的前后发生了大于15％的变化,分别是Akt与pAkt（S473）,mTOR与pmTOR,ERK2,p-p38,pSTAT3（Y705）与pSTAT3（S727）及Rb与p—Rb。结论14-3-3σ在鼻咽癌CNE．2细胞中所起的作用可涉及4条信号通路,分别是Akt通路、MAPK通路、JAK-STAT通路及Rb—E2f通路。     

Naloxone blocks the analgesic action of levorphanol but not of dextrorphan in the leopard frog

Fluorescent dyes Fast Blue and Nuclear Yellow injected into pairs of visual cortical and parietal 'association' cortical areas in the cat revealed the presence of retrogradely double-labeled cells in the intralaminar nuclei and lateral posterior-pulvinar complex of the thalamus. These results demonstrate the projection of individual thalamic neurons onto multiple cortical areas.