小叶锦鸡儿的抗炎作用

小叶锦鸡儿可明显对抗角叉菜胶、热烫及巴豆油所致的炎症,抑制小鼠毛细血管通透性、单核巨噬细胞系统的吞噬功能、肉芽组织增生及大鼠炎症部位PGE₂的合成和释放。     

菝葜乙酸乙酯提取物抗癌活性的实验研究

目的 菝葜属百合科植物,为一种常用中药,目前所用的菝葜提取物多为其40%的乙醇提取物,本实验室首创了菝葜的乙酸乙酯提取工艺并对其进行了抗癌活性研究,旨在探讨其抗肿瘤作用及机制.方法 小鼠体内抗肿瘤试验和MTT法检测菝葜乙酸乙酯提取物在体内外对肿瘤细胞生长的抑制.结果 菝葜乙酸乙酯提取物可以在体内外有效抑制肿瘤细胞的增殖,对小鼠S180、H22、EAC肿瘤细胞及HepG2细胞株的半数抑制浓度(IC50)分别为(0.521±0.272)g/L、(0.801±0.333)g/L、(0.512±0.217)g/L、(0.608±0.268)g/L.结论 菝葜乙酸乙酯提取物具有抗肿瘤作用,具有临床应用的价值.     

小叶锦鸡儿中异黄酮及其苷类化合物的体外抗肿瘤活性研究

目的研究小叶锦鸡儿中异黄酮及其苷类化合物的体外抗肿瘤细胞增殖作用。方法利用四唑盐比色法检测,观察小叶锦鸡儿中分离出的3种异黄酮及其苷类化合物:雁靛黄素(Ⅰ)、高丽槐树-7-O-β-D-吡喃葡萄糖苷(Ⅱ)和芒柄花素-7-O-β-D-吡喃葡萄糖苷(Ⅲ)对人乳腺癌细胞株、宫颈癌细胞株、人肝癌细胞株生长的影响。结果化合物Ⅰ对人乳腺癌细胞株、宫颈癌细胞株的增殖均有明显的抑制作用,IC50分别为20.2、16.9μg.mL-1;化合物Ⅱ对乳腺癌细胞株、肝癌细胞株均有不同强度的抑制作用,IC50分别为17.9、18.8μg.mL-1;化合物Ⅲ对乳腺癌细胞株的增殖有抑制作用,但不呈现浓度依赖关系,对其他两个细胞株的增殖不显示抑制活性。结论化合物Ⅰ、Ⅱ对两种细胞株有不同程度的抗肿瘤活性,化合物Ⅲ不具抗肿瘤活性。     

复方金刚藤治疗盆腔慢性炎症性包块30例临床分析

采用复方金刚藤（金刚藤，大红藤，两面针，白花蛇舌草）治疗盆腔慢性炎症性包块３０例，有效率达９７％，与西药组３３例对照，经Ｒｉｄｉｔ检验，ｔ＝２．９４３，Ｐ〈０．１，２组疗效比较有显著性差异。复方金刚藤治疗效果明显优于西药，且无毒副作用，价格低廉，便于推广应用。     

黑刺菝葜根脂溶性化学成分研究

目的：研究百合科菝葜属植物黑刺菝葜（Smilax scobinicaulis C.H.Wright）根脂溶性化学成分。方法：采用硅胶柱色谱、HP-20和Sephadex LH-20等分离手段对黑刺菝葜根脂溶性化学成分进行分离纯化,通过波谱数据分析（1H-NMR、13C-NMR）进行结构鉴定。结果：从脂溶性化学成分分离7个化合物。分别鉴定为：（1）β-sitosterol,（2）2,4-dihydroxy-3,6-dimethylbenzoate,（3）1-O-p-coumaroylglycerol,（4）ursolic acid,（5）blumenol A,（6）clemaphenolA,（7）1-O-feruloylglycerol。结论：化合物2~7为首次从该植物中分离得到。     

响应面法优化豆腐柴中总黄酮提取工艺

目的研究豆腐柴中总黄酮的最佳提取工艺及其抗菌作用效果。方法以提取时间、料液比、乙醇浓度分数作为自变量,以豆腐柴中总黄酮（按芦丁计）的提取率作为因变量,采用响应面法优选总黄酮的最佳提取工艺,并采用紫外分光光度法测定豆腐柴中总黄酮的含量。结果确定最优提取工艺为提取时间为25.95 min,料液比为1∶24.05,乙醇浓度为81.89%,提取次数为3次,在此条件下,豆腐柴中总黄酮得率理论上可达0.807%。结论星点设计-效应响应面法优选豆腐柴中总黄酮类的提取工艺,方法简单,结果可靠。     

Effects of Crataegus microphylla on Vascular Dysfunction in Streptozotocin‐induced Diabetic Rats

Vascular dysfunction plays a key role in the pathogenesis of diabetic vascular disease. In this study, we aimed to investigate whether chronic in vivo treatment of Crataegus microphylla (CM) extract in diabetic rats induced with streptozotocin (STZ, intraperitoneal, 65 mg/kg) preserves vascular function and to evaluate whether the reduction of inducible nitric oxide synthase (iNOS), proinflammatory cytokines, and lipid peroxidation mediates its mechanisms of action. Starting at 4 weeks of diabetes, CM extract (100 mg/kg) was administrated to diabetic rats for 4 weeks. In aortic rings, relaxation to acetylcholine and vasoreactivity to noradrenaline were impaired, whereas aortic iNOS expression and plasma tumor necrosis factor‐α (TNF‐α) and interleukin‐6 (IL‐6), total nitrite–nitrate, and malondialdehite levels were increased in diabetic rats compared with controls. Chronic CM treatment significantly corrected all the above abnormalities in diabetic rats. In comparison, pretreatment of the aorta of diabetic rats with N‐[3(aminomethyl) benzyl]‐acetamidine, dihydrochloride (10^–5 M), a selective inhibitor of iNOS, produced a similar recovery in vascular reactivity. These results suggest that chronic in vivo treatment of CM preserves endothelium‐dependent relaxation and vascular contraction in STZ‐induced diabetes, possibly by reducing iNOS expression in the aorta and by decreasing plasma levels of TNF‐α and IL‐6 and by preventing lipid peroxidation. Copyright © 2012 John Wiley & Sons, Ltd.     

皖南歙县药用植物分布新记录

目的 摸清皖南野生药用植物分布的新种类,为后期安徽省药用植物资源种类的统计奠定基础.方法 采用野外调查、文献查阅和标本鉴定的研究方法.结果 皖南有4种药用植物分布新记录,新记录种分别是锦葵科植物白背黄花稔Sida rhombi folia Linn.、景天科植物土佐景天Sedum tosaense Makino、大戟科植物飞扬草Euphorbia hirta Linn.和荨麻科植物小叶冷水花Pilea microphylla (Linn.) Liebm..结论 这4种药用植物标本可为其在安徽的分布提供依据,可丰富安徽药用植物资源的种类.     

Effects of Total Alkaloids in Buxus microphylla Leaves on Aorta Smooth Muscle of Rats and Their Mechanisms

Objective To investigate the effects of total alkaloids in Buxus microphylla leaves(ABML)on isolated rats thoracic aorta rings,and then to explore the possible mechanisms underlying the effects.Methods Thoracic aortas of Wistar rats were isolated,removed,and mounted onto an organ bath.The effects of ABML at different concentration on the contraction of isolated thoracic aorta rings(with and without endothelium)precontracted with KCl or PE were observed with organ bath technique.Dose-effect curves of CaCl2 were recorded by organ bath technique.The concentration of intracellular Ca 2+ ([Ca 2+ ]i)increased by PE,KCI,and caffeine in the presence of ABML was determined using Ca 2+ sensitive fluorescence indicator Fura-2/AM loaded thoracic aorta vascular smooth muscle (VSM)cells of rats.Results In aorta rings precontracted with PE and KCl,ABML produced concentration- dependent relaxation in both intact and denuded endothelium ring groups.There was no difference in the inhibition of contraction between the intact and denuded endothelium ring groups at the same concentration.Exposure of isolated thoracic aorta rings to ABML led to a significant reduction in the contracting response induced by CaCl2,and shifted the cumulative concentration-response curves to right.ABML could significantly inhibit the extracellular Ca 2+ influx induced by PE and KCl under[Ca 2+ ]0 of 1.5 mmol/L,with inhibitory ratios of 40.2%and 49.9%,respectively.In the case of Ca 2+ -free,ABML could significantly inhibit the intracellular Ca 2+ release induced by PE,with inhibitory ratio of 72.4%.Conclusion ABML relaxes thoracic aorta VSM cells by suppressing influx of extracellular Ca 2+ via voltage-dependent Ca 2+ channel and receptor-operated Ca 2+ channel.     

赤土茯苓甙的分离和结构鉴定

目的：鉴定湖南产赤土茯苓的乙醇提取物的化学结构。方法：经溶剂粗分和聚酰胺柱层析,得到一单体黄酮类化合物,并对其采用化学分析和ＵＶ、ＩＲ、ＮＭＲ和ＭＳ等光谱分析。结果：经综合解析和立体结构分析,确证其与落新妇甙的结构一致。本研究的１ＨＮＭＲ数据提供了详细信息（各羟基质子的δ值）。且首次测得其质谱数据,对确定其结构提供了强有力的结构解析依据。结论：分离和鉴定了赤土茯苓甙的化学结构为５ ,７,３＇,４＇一四羟基二氢黄酮醇－３ －０－ α－ Ｌ鼠李糖甙。