14岁以下正常人群B19病毒抗体的测定

用间接免疫荧光法检测了延边地区１４岁以下不同年龄组的正常人群血清中抗Ｂ１９病毒抗体。结果如下：抗体阳性率为１２．９％，各年龄组抗体阳性率分布不均，主要在７．５％～２２．５％之间，几何平均效价为１：１９，各年龄组的几何平均效价在１：１６～１：２５之间；抗体效价分布在１：１０～１：８０之间，主要在１：４０以下，朝鲜族和汉族的病毒抗体阳性率分别为９．７％和１６．０％，几何平均效价分别为１：２１和１：１８．统计结果表明，各年龄组之间及朝鲜族和汉族之间，抗体阳性率和几何平均效价均无显著性差异（Ｐ＞０．０５）．     

Detection and viral nucleotide sequence analysis of transfusion-transmitted virus infection in acute fulminant and non-fulminant hepatitis

The impact of transfusion-transmitted virus (TTV) infection on acute fulminant and non-fulminant hepatitis is unclear. In this study, serum samples from 164 patients with acute hepatitis of various aetiologies, from 34 asymptomatic hepatitis B virus carriers and from 202 healthy adults were tested for TTV DNA by the semiconserved nested polymerase chain reaction. TTV viraemia was prevalent in patients with acute hepatitis C, in patients with acute hepatitis D virus superinfection and in patients with non-A–E hepatitis (27–30%) but the incidence was not significantly different from that of healthy controls (31 of 202, 15.3%). There were no significant differences in gender, age, presence of hepatitis G virus, the occurrence of fulminant hepatitis, or in serum albumin, bilirubin or alanine aminotransferase levels (9/30 vs 35/134) between patients with or without TTV viraemia. Seven of the nine TTV-positive patients with fulminant hepatitis were co-infected with hepatitis C, D or E. TTV clones from 18 subjects, with or without fulminant hepatitis, were sequenced and analysed phylogenetically. Eleven (61.1%) belonged to TTV group 1, six (33.3%) to TTV group 2 and one to TTV group 3. No particular strain of TTV was associated with fulminant hepatitis. In summary, in Taiwan, TTV is prevalent in the general population as well as in patients with liver diseases. TTV plays an insignificant role in acute fulminant and non-fulminant hepatitis. Its influence on outcome requires a longitudinal study.     

Preservation of GLUT 2 expression in islet beta cells of Kilham Rat Virus (KRV) — infected diabetes-resistant BB/Wor rats

Summary Loss of GLUT 2, the glucose transporter isoform of pancreatic beta cells, has been reported to accompany the onset and perhaps contribute to the pathogenesis, of insulin-dependent and non-insulin-dependent diabetes mellitus in BB/Wor and Zucker fatty rats. In this study we investigated the effect of Kilham Rat Virus infection on GLUT 2 expression in diabetes-resistant BB/Wor rats. Viral antibodyfree diabetes-resistant rats do not develop spontaneous diabetes, but inoculation with Kilham Rat Virus induces autoimmune beta-cell destruction and hyperglycaemia. Pancreas sections from normoglycaemic diabetes-resistant BB/Wor rats were obtained 5, 7 and 25 days after inoculation with Kilham Rat Virus and stained for GLUT 2 using a rabbit polyclonal antibody. At all time points, beta cells displayed GLUT 2 expression comparable to uninfected diabetes-resistant controls. Immunostained insulin content of the beta cells also remained unchanged. Sections were also examined from Kilham Rat Virus inoculated diabetes-resistant rats with lymphocytic insulitis or diabetes. GLUT 2 and insulin immunostaining were unchanged in non-diabetic rats with early insulitis. GLUT 2 beta-cell staining was variably reduced in diabetic rats with established insulitis and reduced beta-cell insulin immunostaining. Hence, the initial stages of Kilham Rat Virus-induced diabetes in diabetes-resistant rats are not accompanied by a significant reduction in GLUT 2 expression. These results suggest that the loss of GLUT 2 does not play a significant role in the aetiology of diabetes in the Kilham Rat Virus-infected diabetes-resistant BB/Wor rat.Abbreviations KRV Kilham Rat Virus - NHS normal horse serum - BB/Wor Biobreeding/Worcester rats     

细小病毒B19宫内感染与新生儿贫血的相关性研究

目的探讨细小病毒B19宫内感染与新生儿贫血的相关性。方法收集138例贫血新生儿和85例未贫血新生儿的脐血,应用酶联免疫吸附试验检测两组患儿脐血中的人细小病毒B19IgM抗体水平。结果两组患儿脐血检测结果差异具有统计学意义(P0.05)。结论 B19病毒宫内感染与新生儿贫血具有一定的相关性。     

急性呼吸道感染患儿人类博卡病毒检测与分析

目的 探讨人类博卡病毒（HBoV）与儿童急性呼吸道感染（ARTI）的关系。方法 RT-PCR检测2006年11月-2008年4月连续2个冬春季儿科418例呼吸道感染鼻咽分泌物标本的HBoV。结果 HBoV阳性率为5．0％（21／418）。阳性者年龄在4月～5岁。下呼吸道感染HBoV阳性率11．1％（17／153）显著高于上呼吸道感染的1．5％（4／265）（χ^2=18．741,P〈0．001）。结论 HBoV是本地区冬春季儿童呼吸道感染、尤其是下呼吸道感染的的重要病原之一。