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排序方式: 共有593条查询结果,搜索用时 31 毫秒
1.
目的 检测亚硒酸钠是否能够削弱或解除顺铂对植物血球凝激素(PHA)刺激的人体外周血淋巴细胞的增殖抑制。方法 用顺铂和亚硒酸钠单独或联合处理PHA刺激的人体外周血淋巴细胞,顺铂(0.05,0.20,0.50mg/L)在细胞培养24h时加入,亚硒酸钠(0.05mg/L)在不同处理中分别在细胞培养开始时加入或与顺铂同时加入;培养72h后检测转化淋巴细胞的有丝分裂指数。结果0.05mg/L亚硒酸钠在培养开始时加入,PHA刺激转化的淋巴细胞有丝分裂指数较对照增长42.8%(P<0.05),与顺铂同时加入,有丝分裂指数增长13.7%(P>0.05)。0.05与0.20mg/L顺铂处理细胞,有丝分裂指数未发生显性改变,当顺铂剂量为0.50mg/L时,细胞有丝分裂指数较对照降低54.5%(P<0.001)。亚硒酸钠预处理细胞可以解除0.50mg/L顺铂所致的细胞增殖抑制,使有丝分裂指数恢复正常,但亚硒酸钠与顺铂同时加入时,被顺铂抑制的有丝分裂指数只能部分提高。结论0.05mg/L亚硒酸钠单独作用于淋巴细胞可直接促进细胞增殖,与顺铂联合处理细胞时,可降低顺铂毒性,拮抗顺铂的抗增殖作用。亚硒酸钠在细胞培养开始时加入培养体系效果更佳。 相似文献
2.
N. Ya. Popova A. I. Antokhin N. V. Adrianov L. Z. Tret'yakova Yu. A. Romanov 《Bulletin of experimental biology and medicine》1991,112(4):1486-1488
Department of Biology, Medico-Biological Faculty, and Applied Research Laboratory of Ecology, Toxicology, and Metabolism of Medicinal Preparations, attached to the Department of Biochemistry, Medico-Biological Faculty, N. I. Pirogov Second Moscow Medical Institute. Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 112, No. 10, pp. 418–419, October, 1991. 相似文献
3.
目的:观察丙氨瑞林对子宫内膜癌的治疗作用。方法:对诊刮确诊的17例子宫内膜癌病人,予丙氨瑞林700μg,im,qd×7d,用药前后作性激素测定及组织学检查。结果:绝经妇女用药后血中FSH和LH下降(P<0.01和<0.05),雌二醇、孕酮及睾丸酮变化不明显。其中13例内膜样腺癌用药后有丝分裂指数明显下降(P<0.01),有丝分裂指数变化与癌周内膜之反应有关。呈增生反应癌周内膜癌组织有丝分裂指数下降值明显高于呈萎缩状态癌周内膜。结论:丙氨瑞林治疗子宫内膜癌后能降低FSH和LH水平,对子宫内膜癌尤其是雌激素依赖性内膜癌有一定疗效。 相似文献
4.
5.
T. I. Shklyar 《Bulletin of experimental biology and medicine》1977,84(5):1631-1632
Wounds 1 cm long, down to the cutaneous muscle, were inflicted on rats aged 11 days, 2 months, and 2.5 months in the subscapular region. Nociceptive stimulation was applied to the animals by means of an electric current 48 h after the operation. Mitotic activity was determined in cells of the stratum basale of the skin epithelium adjacent to the wound. Nociceptive stimulation was found to reduce the mitotic index of the cutaneous epithelial cells of the animals of all age groups studied by a considerable degree (by several times). An increase in the mitotic index was observed from the early to the later stages of postnatal ontogeny.Laboratory of Age and Evolutionary Physiology, Voroshilovgrad Pedagogic Institute. Laboratory of Growth and Development, Institute of Human Morphology, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR A. P. Avtsyn.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 84, No. 11, pp. 605–606, November, 1977. 相似文献
6.
Changes in the weight and mitotic index in the thymus cortex of Wistar rats were studied during hypokinesia for 10 days followed by recovery for the same period. The mitotic index was reduced by half, 24 h after immobilization of the animals. During readaptation a stage of secondary stress (when the mitotic index was reduced by 71%) was followed by a stage of true readaptation after 10 days.Institute of Human Morphology, Academy of Medical Sciences of the USSR, Moscow. Institute of Marine Biology, Vladivostok. (Presented by Academician of the Academy of Medical Sciences of the USSR A. P. Avtsyn.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 88, No. 10, pp. 480–482, October, 1979. 相似文献
7.
In vivo requirements for rDNA chromosome condensation reveal two cell-cycle-regulated pathways for mitotic chromosome folding 总被引:4,自引:1,他引:4
Chromosome condensation plays an essential role in the maintenance of genetic integrity. Using genetic, cell biological, and biochemical approaches, we distinguish two cell-cycle-regulated pathways for chromosome condensation in budding yeast. From G(2) to metaphase, we show that the condensation of the approximately 1-Mb rDNA array is a multistep process, and describe condensin-dependent clustering, alignment, and resolution steps in chromosome folding. We functionally define a further postmetaphase chromosome assembly maturation step that is required for the maintenance of chromosome structural integrity during segregation. This late step in condensation requires the conserved mitotic kinase Ipl1/aurora in addition to condensin, but is independent of cohesin. Consistent with this, the late condensation pathway is initiated during the metaphase-to-anaphase transition, supports de novo condensation in cohesin mutants, and correlates with the Ipl1/aurora-dependent phosphorylation of condensin. These data provide insight into the molecular mechanisms of higher-order chromosome folding and suggest that two distinct condensation pathways, one involving cohesins and the other Ipl1/aurora, are required to modulate chromosome structure during mitosis. 相似文献
8.
V. I. Shumakov V. N. Blyumkin R. A. Babikova V. P. Fedotov G. G. Tsertsvadze N. V. Sadovnikova L. I. Ul'yanova 《Bulletin of experimental biology and medicine》1979,88(2):903-905
Cultures of islets cells were obtained from the cadaveric pancreas of 16–25-week human fetuses. During culture two waves of mitotic activity were observed. An increase in the insulin concentration in the culture medium took place after a wave of mitotic activity.Department of Artificial Organs and Assisted Circulation, Laboratory of Pathomorphology, Scientific-Research Institute of Transplantation and Artificial Organs, Ministry of Health of the USSR. Laboratory of Biological Standardization of Hormones, Institute of Experimental Endocrinology and Hormone Chemistry, Academy of Medical Sciences of the USSR. Maternity Home No. 26, Moscow. Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 88, No. 8, pp. 202–204, August, 1979. 相似文献
9.
10.
Yu. A. Romanov T. N. Ivchenko Z. F. Sultanova 《Bulletin of experimental biology and medicine》1988,105(3):408-411
Department of Biology, Medico-Biological Faculty, N. I. Pirogov Second Moscow Medical Institute. Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 105, No. 3, pp. 345–347, March, 1988. 相似文献