Experimental Study on the Inhibitory Effects of Verapamil on the Proliferation of Meningiomas Cells

In order to investigate the effects of verapamil on the proliferation of meningiomas cells in vitro and in vivo, the cultured meningiomas cells were cultured with verapamil at different concen-trations for 24 h and the inhibitory effects of verapamil on cell proliferation were observed by MTT method. The meningiomas model was established by implanting the newly removed tumor fragments into the nude mice subcutaneously. The nude mice with tumors were divided into two groups: vera-pamil-treated group and control group. Tumor volumes were measured and after 12 weeks the tumors were taken out and examined histologically. The expression of proliferating cell nuclear antigen (PCNA) in the tumors was detected by using immunohistochemistry. It was found that verapamil could inhibit the growth of cultured meningiomas cells in a concentration-dependant manner. The in-hibitory effect could be observed in the concentration of 1 μmol/L verapamil and the most obvious effects appeared in the concentration of 100 μmol/L. Tumor volume in the verapamiltreated group was obviously smaller than that in the control group (211.40±5.50 vs 163.94±3.62, P<0.01) and the expression of PCNA was also lower (1.52±0.24 vs 2.86±0.53, P<0.05). Tumor inhibition rate was about 22.45%. It was suggested that verapamil could inhibit the proliferation and growth of men-ingiomas cells in vitro and in vivo.     

小脑幕脑膜瘤的显微手术治疗

目的：总结小脑幕脑膜瘤显微手术的经验和教训，以利提高手术的疗效。方法：采用经颞枕硬膜内入路，枕部经小脑幕与枕下联合入路，枕下内侧入路、旁正中入路，均采用显微神经外科技术切除肿瘤。结果：肿瘤全切除6例，次全切除2例，术后死亡1例，共济失调1例，构音障碍和饮水咳呛1例，5例痊愈。结论：小脑幕脑膜瘤切除应选择合适的手术入路。手术时必须切除窦内肿瘤，在切除肿瘤侵入的横窦前、必须有血管造影完全闭塞的证据。脑干随近的肿瘤先行包膜内切除，后切除肿瘤包膜。