复合磷脂和大豆磷脂对大鼠血脂和心肌丙二醛及脉搏波的影响

 目的：观察复合碑脂和大豆磷脂对高脂大鼠血脂、心肌丙二醛及脉搏波的影响。方法：在高脂饲料中添加复合磷脂或大豆磷脂饲养大鼠，3周末浏定血脂、心肌丙二醛及脉搏波。结果：复合碑脂和大豆磷脂都能明显降低大鼠血清胆固醉、甘油三脂及低密度脂蛋白胆固醉的含蚤；复合碑脂还可明显降低心肌丙二醛含t，提高尾动脉脉搏波上升支针率，增加降中峡的相对深度。结论：复合碑脂和大豆磷脂都具有降血脂的作用;复合碑脂还具有延缓衷老的作用。     

Role of Lipid Peroxidation in Bacterial Translocation from the Small Intestine after Thermal Injury in Rats

Oxygen-derived free radicals have bran proved to play a role in the intestinalmucosa damage induced by ischemic reperfusion after burns,but their role in thebacterial translocation from the intestine to other organs is not documented so far.Theauthors intended to investigate this problem on 186rats,which were randomized into 5groups:the normal control group,the group receiving oral Pseudomonas,the group ofsimple burn injury,and the groups of combined oral Pseudomonas and burn injury withor without superoxide dismutase(SOD)treatment.The burn injury was 30% TBSA fullthickness scalding.The oral administered Pseudomonas was labdled with isothiocyanatefor tracing.The animals were killed in the 4th,12th,24th,48th,or 72nd hour afterinjury respectively.The changes of malondialdehyde(MDA)in the ilcal mucosa were ob-served with optical and electron microscope,and bacterial translocation from the intes-tine to the liver and blood stream was traced .The animals with combined injury and simple burns showed a marked increase ofileal mucosal MDA with the peak in the 12th hour accompanied with intense pathologicalchanges in the small intestine;the incidence of bacterial translocation from the intestineto other organs also increased.With SOD treatment,the MDA levd in the ileal mucosawas significantly lower,the pathological changes in the intestinal mucosa were allevia-ted,and the incidence of bacterial translocation from the intestine was lowered with nolabdled Pseudomonas isolated from the blood stream.In this study,it was demonstrated that free radicals are one of the factors ofintestinal mucosal damage and bacterial translocation from the intestine after burns,andSOD could protect the intestinal mucosal barrier from being injured and inhibit thebacterial translocation from the intestine.     

葱白提取物对急性心肌缺血大鼠抗氧化作用的影响

目的：观察葱白提取物对急性心肌缺血大鼠抗氧化作用的影响。方法：将健康雄性SD大鼠40只,按体质量排序以随机数字表法分为假手术组、空白模型组、葱白提取物组和硝酸甘油组,每组各10只。成功制备急性心肌缺血模型后5min于十二指肠内给药,给药体积为2mL·kg-1。假手术组和空白模型组给与同等体积生理盐水,葱白提取物组按600mg·kg-1给药、硝酸甘油组按80mg·kg-1给药。结果：与假手术组比较,空白模型组大鼠ST段明显升高（P〈0．01）;与空白模型组比较,硝酸甘油组、葱白提取物组均可降低大鼠sT段（P〈0．01）,葱白提取物与硝酸甘油作用相当（P〉0．05）。与空白模型组心肌梗死面积及T—AOC比较,硝酸甘油组、葱白提取物组有显著差异（P〈0．01）,葱白提取物与硝酸甘油作用相当（P〉0．05）。与空白模型组SOD比较,硝酸甘油组、葱白提取物组有显著差异（P〈0．01）,葱白提取物作用弱于硝酸甘油（P〈0．05）。与空白模型组MDA比较,硝酸甘油组、葱白提取物组有显著差异（P〈0．01）,葱白提取物作用优于硝酸甘油（P〈0．05）。结论：葱白提取物可降低大鼠sT段,减少心梗面积,显著降低大鼠血清中MDA、升高SOD及T—AOC水平,对缺血心肌具有保护作用。     

美满霉素对脑缺血再灌注大鼠血清SOD和MDA的影响

目的 观察美满霉素(minocycline)对脑缺血再灌注大鼠血清超氧化物歧化酶(SOD)、丙二醛(MDA)浓度的影响,探讨美满霉素清除脑缺血再灌注后的自由基的作用.方法 Wistar大鼠随机分为:正常组(N组)、缺血再灌注组(IR组)、假手术组(NS组)、美满霉素对照组(M组)、美满霉素治疗组(MT组)、美满霉素与处理组(MP组).处死大鼠取样,采用化学比色法检测血清SOD和MDA浓度;HE染色观察大鼠脑组织的病理改变.结果 MP、 MT组SOD浓度较IR组明显增高,差异有统计学意义(P＜0.01);MP、 MT组MDA浓度较IR组显著降低,差异有统计学意义(P＜0.01).结论 美满霉素能使大鼠脑缺血再灌注后血清SOD浓度增高、MDA浓度降低,美满霉素可以通过增强自由基清除发挥脑保护作用.