秦岭产宜昌蛇菰生药学特征与抗氧化活性研究

目的：对秦岭产宜昌蛇菰的生药学特征进行鉴别研究,为品种鉴定提供参考依据;对宜昌蛇菰体外抗氧化活性进行研究,为有效利用其资源提供参考依据。方法对原植物形态、性状、根茎、鳞叶、横切面显微进行鉴别研究;借助紫外分光光度法,通过比较1,1-二苯基-2-三硝基苯肼（DPPH）清除率,评价宜昌蛇菰各部位不同溶剂提取物的体外抗氧化活性。结果宜昌蛇菰的茎横切面表皮以内为薄壁组织,外韧性维管束散在,无明显皮层、中柱以及髓部分化;鳞叶横切面可见主脉维管束散在,无栅栏组织、海绵组织的分化,呈现等面叶的特征。宜昌蛇菰各部位不同溶剂提取液在实验质量浓度范围内,各提取物抗氧化能力均随其质量浓度升高而增强。结论宜昌蛇菰花茎和鳞叶横切面初生构造的特征,对其品种鉴定提供参考依据。宜昌蛇菰根茎、鳞叶、花均具有明显抗氧化活性,三者间无明显差异,故提示其应全株入药。     

Alkaloids from Melodinus henryi with anti-inflammatory activity

One new quinolinic scandine-type monoterpeniod alkaloid, 3-oxo-scandine (1), as well as seven known ones (2–8), was isolated from the roots of Melodinus henryi. Their structures were elucidated by extensive spectroscopic analysis. All of the compounds were prepared and evaluated for their anti-inflammatory activities by measuring the inhibitory activity of nitric oxide (NO) in vitro in RAW 264.7 mouse peritoneal macrophages. Compounds 6 and 7 showed significant activities with IC₅₀ values of 8.54 and 5.19 μM, respectively.     

RUSSELL GRIMWADE PRIZE

The only valid reason for the state to invest in forest growing is to provide or protect social benefits which are considered important but which would not otherwise be adequately or fairly supplied through private forestry. Such multiple objectives can be met by exploiting the joint production possibilities of forest growth, but the returns are a complex mixture of quantifiable, partly quantifiable and unquantifiable values. One of the quantifiable outputs is wood, and the returns from it can be measured in financial terms. But in growing forests for social purposes wood is really a by-product or a means of achieving certain social purposes. Attempts therefore to assess the adequacy of the returns to the state as a forest grower in terms of the revenue from timber growing are of doubtful logic and so fraught with difficulties, as to be not worth the effort. Concern would be more usefully directed toward the costs of producing the multiple returns of state forestry.     

Triterpenoids from<Emphasis Type="Italic">Schisandra henryi</Emphasis> with cytotoxic effect on leukemia and hela cells<Emphasis Type="Italic">in vitro</Emphasis>

Four known lanostane triterpenoids, schiprolactone A (1), schisanlactone B (2), nigranoic acid (3) and schisandronic acid (4) were isolated from the stems of Schisandra henryi for the first time. Their structures were characterized by IR, MS and NMR techniques. Compounds 1, 2 and 4 showed moderate cytotoxic activity against Leukemia cells in vitro. Cytotoxic activity of compounds 1-4 showed IC50 of 0.0097, 0.01, 0.097 and 0.0099 micromol/mL respectively toward Leukemia cells and IC50 of 0.097, 0.1, 0.097 and 0.099 micromol/mL toward Hela cells respectively. It is the first report that these compounds possess cytotoxic activity on Leukemia and Hela cells.     

大孔吸附树脂分离纯化单叶铁线莲总皂苷的研究

目的研究大孔吸附树脂分离纯化单叶铁线莲总皂苷的工艺条件,为单叶铁线莲总皂苷的工业化生产提供实验依据。方法采用比色法测定总皂苷;比较了5种大孔吸附树脂的静态饱和吸附量、静态解吸率,筛选出适宜的树脂;对最佳树脂进行动态吸附、解吸试验,确定最佳的吸附洗脱工艺。结果 D-101大孔树脂对单叶铁线莲总皂苷有较好的吸附分离性能。最佳工艺条件为:上样液质量浓度为生药0.2 g/mL,pH值为5,上样体积流量为1 BV/h;4 BV蒸馏水洗脱杂质;4 BV 70%乙醇洗脱总皂苷,洗脱体积流量2 BV/h。按此工艺条件生产的总皂苷质量分数达到71.53%,收率86.02%,精制度达238.81%。结论 D-101大孔吸附树脂适合富集纯化单叶铁线莲中总皂苷。     

红鸡踢香的显微结构研究

[目的]为进一步开发利用、制订红鸡踢香质量标准提供参考依据。[方法]采用永久性固定制片法和粉末制片法。[结果]其根部皮层石细胞、纤维众多；茎的中柱鞘纤维成环；叶的中脉外侧有纤维束环；叶肉有石细胞、纤维散在；粉末中石细胞众多，孔沟明显；可见草酸钙方晶。[结论]红鸡踢香的显微结构可为其进一步开发、制订质量标准提供参考依据。     

Nine new compounds from the whole plants of Rehmannia henryi

Eight new iridoids (1–8) and an ionone glucoside (9), together with 31 known compounds (10–40), were isolated from the whole plants of Rehmannia henryi. The structures of these compounds were elucidated on the basis of their spectroscopic data and chemical evidence.     

RP-HPLC测定糙叶五加不同药用部位中槲皮素和山柰酚

目的 建立同时测定糙叶五加不同药用部位中槲皮素和山柰酚的方法.方法采用RP-HPLC法,色谱柱为AT.Lichrom ODS-C18柱(250 mm×4.6 mm,5μm),以甲醇-磷酸-水(50∶0.2∶49.8)为流动相,柱温30℃,体积流量1.0 mL/min,检测波长360 nm,进样量10μL.结果槲皮素在0...     

女贞子多糖抗去势小鼠骨质疏松作用研究

[目的]研究女贞子多糖对去卵巢所致小鼠骨质疏松的影响.[方法]给予去卵巢小鼠女贞子多糖(3 g/kg和6 g/kg,以生药计)8周后取材,用双能射线骨密度仪和微计算机断层扫描技术(micro CT)分析小鼠股骨骨密度和股骨干骺端骨微结构,全自动生化仪检测血浆中钙(Ca)和无机磷(P)水平.体外培养骨髓来源单个核细胞,使...     

二至丸的现代研究及在老年性黄斑变性中的应用

目的:总结近年来的研究进展,为二至丸治疗老年性黄斑变性(AMD)提供借鉴和依据。方法:查阅二至丸及AMD的大量文献报道,总结分析。结果:二至丸中女贞子、墨旱莲具有抗衰老、调节免疫力、降糖降脂、抗凝、抗炎抑菌等作用。在临床上常作为基础方治疗多种疾病。结论:二至丸加味治疗AMD既有充足的理论依据,又有良好的临床疗效验证,在临床上值得推广。