瘤内注射pSTbAT-脂质体复合物对裸鼠移植瘤抑制效应的实验研究

目的:构建血管生成抑制因子arresten基 因的真核表达载体,并观察瘤内注射质粒DNA 脂质 体复合物对裸鼠移植瘤生长的影响。方法:利用 PCR方法,由重组质粒pGEM Arr中扩增出基因;将 该基因定向克隆于真核表达载体中。20只裸鼠皮 下注射HepG 2细胞,成瘤后以成功构建的质粒 DNA 脂质体复合物瘤内注射,治疗后采用免疫组化 方法检测肿瘤血管密度,并利用arresten基因瘤内注 射的方法对抑制肿瘤的效果进行分析。结果:我们 成功构建arresten基因真核表达载体(pSTbAT)。实 验组肿瘤生长速度较对照组慢(实验组平均15.3个 阳性血管/10个视野,对照组平均112.5个阳性血 管/10个视野)。结论:瘤内注射arresten基因质粒 DNA复合物能抑制裸鼠移植瘤的血管生成,并能抑 制移植瘤的生长。     

Decreased recurrence of symptoms in oral lichen planus with intralesional injection of hyaluronic acid and triamcinolone

Oral lichen planus (OLP) is a chronic non-infectious, inflammatory, immunological disease. In contrast to skin lesions, which are often self-limiting, oral lesions rarely heal on their own and can be resistant to local and systemic treatments. In this clinical trial, hyaluronic acid (HA) was mixed with triamcinolone for intralesional injection to reduce side effects in the treatment of OLP. This randomized clinical trial with a split-mouth design was performed on 28 patients with OLP. The mouth was divided randomly into two sides: a test side, which received HA combined with triamcinolone, and a control side, which received triamcinolone alone. The rate of symptom recurrence was 74.1% on the control side and 11.1% on the test side (significant difference, P < 0.01). Pain scores did not differ between the two groups when assessed after 2 weeks. The group treated with a combination of HA and triamcinolone experienced a significantly better resolution of lesions and symptoms. Considering the role of HA in tissue healing and in regulating inflammatory responses, as well as its antioxidant and hydration properties, it appears that HA could be effective in improving of OLP and decreasing the rate of symptom recurrence.     

Efficacy of intralesional methotrexate in the treatment of plantar warts

Warts are tumors or growths caused by infection with human papilloma virus (HPV). Currently, over 170 HPV types have been identified. This study aimed to evaluate the efficacy and safety of intralesional injection of methotrexate (MTX) for the treatment of plantar warts. Sixty patients presented with plantar warts were divided into two groups. Group A patients were injected with intralesional MTX (2 mg/ml). Group B patients were injected with intralesional saline as a placebo. The injections were repeated every week for a maximum of six sessions or until complete clearance, whichever was earlier. The patients were followed up for 6 months after the last injection. In the intralesional MTX group, 2 patients (6.7%) showed complete improvement, 8 patients (26.7%) showed partial improvement, and 20 patients (66.7%) showed no improvement. In the intralesional saline group, 3 patients (10%) showed complete improvement, 4 patients (13.3%) showed partial improvement, and 23 patients (76.7%) showed no improvement. Reported adverse events were local reactions in the form of swelling, pain, and infection in both groups. There was no statistically significant difference between the therapeutic responses to intralesional MTX injection and saline.     

Intralesional zinc sulfate 2% vs intralesional vitamin D in plantar warts: A clinicodermoscopic study

Verrucae are benign proliferations seen in skin due to infection with papillomaviruses. There are different treatment strategies for warts but all of these treatments are painful, time consuming, expensive, and recurrence is common. To evaluate and compare the efficacy of intralesional 2% zinc sulfate solution vs vitamin D3 in the treatment of plantar warts, as well as reporting the side effects. This three‐armed randomized clinical trial included 105 patients presented with plantar warts divided into three groups, 35 patients per group. The first group received intralesional 2% zinc sulfate, the second group received intralesional 2% vitamin D3, and the third group received normal saline. Four sessions were done, one every 2 weeks. At the end of the study, patients showing complete response were more in zinc group (71.4%), vitamin D3 group (62.9%) compared to saline group (40%). Most of the patients in zinc group showed severe pain during injection (48.6%), most of the patients in vitamin D3 group showed mild pain (80%), while most of the patients in saline group showed no pain (57.1%). Both intralesional 2% zinc sulfate and vitamin D3 are effective in treatment of plantar warts, with zinc sulfate being more effective.     

Intralesional triamcinolone acetonide in notalgia paresthetica: Treatment outcomes in five patients

Numerous treatment modalities have been tried with diverse results for pruritus due to notalgia paresthetica (NP). Corticosteroids suppress ectopic neural discharges from injured nerve fibers and also have short‐lived suppressive effect on transmission in normal C‐fibers. Herein, we evaluated the efficacy of intralesional triamcinolone acetonide in the treatment of NP. The medical reports of five patients who had been diagnosed with NP and treated with intralesional triamcinolone acetonide injections were retrospectively evaluated. Triamcinolone acetonide solution was injected intradermally (10 mg/mL; 0.1 mL/cm²) every 3 weeks for a maximum of four treatments. The severity of itch was scored by the patients on a combined numerical and visual analogue scale. After treatment, reduction in itch severity scores varied between 33% and 100%.     

复方甘草酸苷联合皮损内封闭治疗结节性痒疹的疗效观察

目的 观察复方甘草酸苷联合皮损内封闭治疗结节性痒疹的疗效.方法 将76例结节性痒疹患者随机分为治疗组与对照组各38例,治疗组给予复方甘草酸苷联合得宝松皮损内封闭治疗,对照组仅给予得宝松皮损内封闭治疗.3个月评定两组患者疗效,6个月随访复发情况.结果 治疗组有效率为89.4％,高于对照组的63.1％（P＜0.05）;治疗组复发率为28.9％,低于对照组的55.2％ （P＜0.05）.结论 复方甘草酸苷联合皮损内封闭治疗结节性痒疹疗效好,复发率低,具有临床应用价值.     

序贯性应用肝动脉化疗栓塞与瘤内无水酒精注射治疗巨块型肝癌144例

 目的 观察经皮肝动脉化疗栓塞（TACE）和CT引导下经皮穿刺瘤内无水酒精（PEI）序贯性治疗巨块型原发性肝癌的疗效。方法 144例无远处转移巨块型原发性肝癌先经TACE术治疗，2周后再进行4 ~ 6次PEI治疗。结果 PR 96例（66.7 %），MR 33例（22.9 %），SD 15例（10.4 %），二期可切除69例，手术切除率47.9 %。随访结果显示：6个月、1、2、3年生存率分别为：93.8 %、75.0 %、59.0 %、31.9 %。结论 TACE与PEI序贯性治疗中晚期巨块型肝癌疗效较显著，使原不能手术切除的肝癌可以适应手术治疗，延长了患者的生存期，提高了患者生活质量