hsacirc0006867在结直肠癌组织中的表达及其临床意义北大核心

目的:探讨环状RNA hsa_circ_0006867在结直肠癌中的表达及其与临床病理因素的关系。方法:全转录组测序筛选结直肠癌中特异circRNAs表达谱,挑选出差异表达显著的hsa_circ_0006867,qRT-PCR检测54例结直肠癌组织及癌旁组织中hsa_circ_0006867表达情况,分析其表达水平与结直肠癌临床病理特征的相关性,ROC曲线分析hsa_circ_0006867在结直肠癌中的诊断价值。结果:测序获得circRNAs在结直肠癌中的差异表达谱,qRT-PCR验证hsa_circ_0006867在结直肠癌中表达下调(P<0.05)。其表达水平与肿瘤分化程度和远处转移有关(P<0.05)。ROC曲线显示hsa_circ_0006867诊断结直肠癌AUC为0.851(95%CI:0.775~0.927),当截断值为0.0146时,敏感度为88.46%(95%CI:0.770~0.946),特异度为73.08%(95%CI:0.598~0.832),差异具有统计学意义(P<0.001)。结论:hsa_circ_0006867在结直肠癌中表达下调,与相关临床病理特征密切联系,可作为潜在结直肠癌临床诊断指标。     

Circular RNA hsa_circ_0000567 can be used as a promising diagnostic biomarker for human colorectal cancer

Background

Recent studies have revealed that circular RNAs are involved in the biological process of some kinds of human cancers. However, little is known about their diagnostic values and functions in colorectal cancer (CRC).

Methods

The expression levels of hsa_circ_0000567 in 102 paired CRC tissues and adjacent noncancerous tissues, 5 CRC cell lines, and a normal colorectal epithelial cell line were detected by quantitative real‐time polymerase chain reaction (qRT‐PCR). The correlations between hsa_circ_0000567 expression levels and the clinicopathological factors of patients with CRC were analyzed. Furthermore, the loss‐of‐function assay was performed to investigate the functions of hsa_circ_0000567 in vitro. Finally, a receiver operating characteristic (ROC) curve was established to evaluate the diagnostic value of hsa_circ_0000567.

Results

Hsa_circ_0000567 expression was significantly downregulated in CRC tissues and CRC cell lines. In addition, the decreased hsa_circ_0000567 expression in CRC was negatively correlated with tumor size (P = .011), lymph metastasis (P = .003), distal metastasis (P < .0001), and tumor–node–metastasis (TNM) stage (P = .003) in CRC. Moreover, knockdown of hsa_circ_0000567 promoted CRC cells proliferation and migration in vitro. Importantly, the area under the ROC curve (AUC) was 0.8653, which indicates hsa_circ_0000567 can serve as a diagnostic biomarker.

Conclusion

Hsa_circ_0000567 may be a novel suppressor and a potential diagnosis biomarker in CRC.

     

Hsa_circRNA_0017620 regulated cell progression of non‐small‐cell lung cancer via miR‐520a‐5p/KRT5 axis

BackgroundCircRNA is a very important functional RNA that plays an important role in the development and metabolism of cancer. However, the study of circRNA in NSCLC has not been fully elucidated.MethodsThe expression of hsa_circ_0017620, SFMBT2, miR‐520a‐5p, and KRT5 was determined using qRT‐PCR. KRT5, Twist1, E‐cadherin, and Ki67 protein expression were measured with western blot. The positive expression rates of Ki67 and Vimentin were determined by immunohistochemistry assay. 5‐Ethynyl‐2’‐deoxyuridine (EdU), colony formation, and MTT assays were used to assess cell proliferation. Transwell migration and invasion assay were applied to determine cell migration and invasion. Dual‐luciferase reporter and RNA immunoprecipitation assays were used to verify the relationship among hsa_circ_0017620, miR‐520a‐5p, and KRT5. The animal experiment was used to ensure the effects of hsa_circ_0017620 on tumor growth in vivo.ResultsHsa_circ_0017620 was upregulated in NSCLC cells and tissues. MiR‐520a‐5p had been verified to be a target miRNA of hsa_circ_0017620 and KRT5 had been verified to be a target mRNA of miR‐520a‐5p in NSCLC cells. Knockdown of hsa_circ_0017620 inhibited cell proliferation, migration, and invasion in NSCLC cells, which was reversed by downregulating miR‐520a‐5p or upregulating KRT5 in NSCLC. Overexpression of hsa_circ_0017620 had opposite effects in NSCLC. Moreover, hsa_circ_0017620 silencing inhibited tumor growth in vivo of NSCLC.ConclusionIn this study, we found that hsa_circ_0017620 played an important role in NSCLC progression. Hsa_circ_0017620 regulated cell proliferation, invasion, and migration through targeting miR‐520a‐5p/KRT5 axis in NSCLC, providing a potential new target for the treatment and diagnosis of NSCLC.     

hsa_circ_0003188对人骨髓间充质干细胞成骨分化能力的影响

目的:探讨hsa_circ_0003188对人骨髓间充质干细胞(hBMSCs)成骨分化能力的影响.方法:Human Circular RNA Micro-array Version 2.0芯片检测正常环境与炎性环境(10 ng/mL TNF-α)培养的hBMSCs差异表达circRNAs,并筛选出目的circRNA-h...     

沉默环状RNA hsa_circ_0001785表达对乳腺癌MDA-MB-231细胞增殖、迁移及侵袭能力的影响

目的:探究环状RNA（circRNA）hsa_circ_0001785在乳腺癌组织和细胞中的表达变化及其对乳腺癌细胞增殖、迁移和侵袭能力的影响。方法:qRT-PCR实验检测hsa_circ_0001785在乳腺癌组织和乳腺癌细胞（MDA-MB-231和SK-BP-3）中的相对表达水平；CCK-8和克隆形成实验检测沉默或上调表达hsa_circ_0001785对MDA-MB-231细胞活性和克隆形成能力的影响；划痕愈合实验和Transwell侵袭实验检测沉默或上调表达hsa_circ_0001785对MDA-MB-231细胞迁移及侵袭能力的影响。结果:hsa_circ_0001785在乳腺癌组织中的相对表达水平明显高于癌旁组织，hsa_circ_0001785在MDA-MB-231和SK-BP-3细胞中的相对表达水平明显高于人乳腺上皮细胞MCF10A。在MDA-MB-231细胞沉默hsa_circ_0001785，MDA-MB-231细胞的活性和克隆形成能力明显降低，迁移距离显著减少，侵袭能力也明显下降。而在MDA-MB-231细胞中上调表达hsa_circ_0001785，MDA-MB-231细胞的活性和克隆形成能力显著升高，迁移距离明显升高，侵袭能力也明显升高。结论:hsa_circ_0001785在乳腺癌组织和乳腺癌细胞（MDA-MB-231和SK-BP-3）中的表达水平明显升高；沉默hsa_circ_0001785显著抑制乳腺癌MDA-MB-231细胞的增殖、迁移和侵袭能力，而上调表达hsa_circ_0001785明显促进乳腺癌MDA-MB-231细胞的增殖、迁移和侵袭能力。