树型金银花无公害优质加工技术的研究

目的探讨树型金银花无公害优质加工技术。方法采用树型金银花晾晒和烘烤以及"烤房3段变温烘烤技术"。结果烘干产品比晒干产品具有明显的质量和价格优势,价格高57.1%。烤房3段变温技术第1段烘干温度控制在35～40℃,烘8～9h;第2段将温度提高到50～55℃左右,烘烤6～8h;第3段温度升到60～70℃,烘烤5～6h左右;烘干总时间不超过24h。结论无公害优质加工技术利于规模化生产,获得无公害的绿色产品,增加金银花附加值。     

Honeysuckle Aqueous Extracts Induced let-7a Suppress EV71 Replication and Pathogenesis In Vitro and In Vivo and Is Predicted to Inhibit SARS-CoV-2

Honeysuckle (Lonicera japonica Thunb) is a traditional Chinese medicine (TCM) with an antipathogenic activity. MicroRNAs (miRNAs) are small non-coding RNA molecules that are ubiquitously expressed in cells. Endogenous miRNA may function as an innate response to block pathogen invasion. The miRNA expression profiles of both mice and humans after the ingestion of honeysuckle were obtained. Fifteen overexpressed miRNAs overlapped and were predicted to be capable of targeting three viruses: dengue virus (DENV), enterovirus 71 (EV71) and SARS-CoV-2. Among them, let-7a was examined to be capable of targeting the EV71 RNA genome by reporter assay and Western blotting. Moreover, honeysuckle-induced let-7a suppression of EV71 RNA and protein expression as well as viral replication were investigated both in vitro and in vivo. We demonstrated that let-7a targeted EV71 at the predicted sequences using luciferase reporter plasmids as well as two infectious replicons (pMP4-y-5 and pTOPO-4643). The suppression of EV71 replication and viral load was demonstrated in two cell lines by luciferase activity, RT-PCR, real-time PCR, Western blotting and plaque assay. Furthermore, EV71-infected suckling mice fed honeysuckle extract or inoculated with let-7a showed decreased clinical scores and a prolonged survival time accompanied with decreased viral RNA, protein expression and virus titer. The ingestion of honeysuckle attenuates EV71 replication and related pathogenesis partially through the upregulation of let-7a expression both in vitro and in vivo. Our previous report and the current findings imply that both honeysuckle and upregulated let-7a can execute a suppressive function against the replication of DENV and EV71. Taken together, this evidence indicates that honeysuckle can induce the expression of let-7a and that this miRNA as well as 11 other miRNAs have great potential to prevent and suppress EV71 replication.     

HPLC法同时检测金银花露中6种防腐剂含量

目的建立了金银花露中6种防腐剂的含量测定方法。方法采用HPLC法。以0．025mol·L-1磷酸二氢钠溶液（磷酸调pH3．7）．甲醇为流动相梯度洗脱,分别在波长228nm、258nm处测定6种防腐剂的含量。结果9个生产厂家的19批金银花露中,有7个生产厂家的16批样品检出苯甲酸,山梨酸,对羟基苯甲酸乙酯的一种或两种。2个生产厂家的3批样品未检测出上述6种防腐剂。结论该方法简便、准确、重现性好,可用于中药液体制剂中常见防腐剂的含量测定。     

忍冬种子萌发期间PAL活性及氯原酸含量变化

忍冬种子萌发期间ＰＡＬ活性呈不断提高趋势，并在胚根与子叶突破或脱离种应时出现两次高峰；氯原酸含量表现为持续提高，提高幅度不断加大。ＰＡＬ活性变化与氯原酸含量变化并不同步但有密切关系。笔者还测定了幼苗不同器官中的ＰＡＬ活性与氯原酸含量。     

金银花修剪技术的研究

本文介绍在平原地区栽培金银花的修剪方法。全银花通过修剪枝条,可培育株型,促发新枝,多结花蕾,一年采收3～4次花。修剪方法如用之适当,可增产18%～21%。     

酸蒸馏—碘滴定法测定金银花中二氧化硫残留量

目的建立酸蒸馏—碘滴定法测定金银花中二氧化硫残留量的方法。方法以金银花为样品,采用酸蒸馏-碘滴定法检测。结果二氧化硫平均回收率为85.82%,RSD为1.04%。结论本方法简便、快速,结果准确,可用于金银花中二氧化硫残留量的检测。     

金银花品种的本草考证

金银花是中国传统的药用品种。该文通过系统地梳理中国历代本草文献中所记载的药用金银花的植物形态,指出传统中医使用的金银花具有缠绕藤本;茎微紫色,有被毛;叶对生,卵状心形,两面皆有被毛;一蒂双花,单花唇形,花冠长3.2 cm左右,花蕊长,花开先白后黄,总花梗单生于小枝叶腋且总苞片叶状卵形,花期3月中旬到5月中旬等特征。在忍冬科各种植物中,仅忍冬科忍冬Lonicera japonica Thunb.一个品种符合传统药用金银花的植物学特征,应作为正品药用金银花的品种使用。     

人工种植金银花中绿原酸含量测定和提取工艺研究

目的测定人工种植金银花中绿原酸的含量和最佳提取工艺。方法采用紫外可见光分光光度法测绿原酸的含量,通过单因素实验和正交实验,优化微波辐射提取金银花中绿原酸的最佳工艺。结果人工种植金银花绿原酸含量为6.40%,最佳提取工艺条件是:微波辐射功率450W,液物料比1∶25,乙醇体积分数70%,微波辐射时间45min,提取溶剂的pH为7。结论人工种植金银花中绿原酸含量较高,完全满足药用。     

The influence of sterilization with EnbioJet Microwave Flow Pasteurizer on composition and bioactivity of aronia and blue-berried honeysuckle juices

In this study, fruit juices that are rich sources of anthocyanins, obtained from aronia (Am. chokeberry, Aronia melanocarpa) and blue-berried honeysuckle (Lonicera caerulea L. var. edulis) were used to examine the preservation of plant phytochemicals and bioactivity upon sterilization—either thermal, or with an EnbioJet^® Microwave Flow Pasteurizer. The chemical properties verified included determinations of anthocyanins and other polyphenols by HPLC, total antioxidant activity, and profiles of antioxidants by post-column derivatization. Compared to heat treatment, the higher stability of aronia and blue-berried honeysuckle phytocomplexes during processing with the EnbioJet^® device for temperatures ensuring microbial purity (range investigated 90-130 °C) was demonstrated. In the same batches of juices submitted to heating at 100 °C, the rapid decline of anthocyanin content accompanied by lowered antioxidant activity was observed. The changes in chemical composition were reflected in altered biological activity. Both cytotoxicity and protection of DNA against oxidative damage were higher for microwaved juices than for juices processed by the heating method that caused degradation of bioactive phytochemicals.