Colonic duplication in an adult mimicking a tumor of pancreas

Duplications of the alimentary tract are uncommon congenital malformations that can present diagnostic difficulties. We report a rare case of a cystic colonic duplication in a female adult. Preoperative investigations were suggestive of pancreatic tumor. The diagnosis was established based on the histopathological examination of the resected specimen. We concluded that, though uncommon, intestinal duplication should be considered in differential diagnosis of abdominal mass.     

多重PCR在瘢痕疙瘩Fas基因突变分析中的应用

目的:建立瘢痕疙瘩Fas基因常见突变外显子的多重PCR扩增反应体系,以利进行批量瘢痕疙瘩基因突变的筛选和检测。方法:取正常皮肤、增生瘢痕和瘢痕疙瘩组织DNA样本,根据瘢痕疙瘩Fas基因易发突变的外显子6,8,9之序列,按照多重PCR引物设计原则,建立3对相应引物,先分别找出各外显子的最佳扩增条件,再进行综合分析,最终找到一个理想的多重PCR扩增条件。结果:各外显子单一扩增片段和多重PCR扩增条带均清晰,产量较高,无非特异性扩增,产物长度与理论值一致;DNA测序证实,各扩增产物即各外显子基因片段。结论:成功建立了瘢痕疙瘩Fas基因常发突变外显子的多重PCR扩增体系,与以往单基因分次扩增相比,实现了一次同时扩增3个基因片段,为瘢痕疙瘩基因诊断和进一步相关的分子生物学研究提供了一个经济、快捷的方法,对于瘢痕疙瘩Fas基因的突变研究具有较高的应用价值。     

Notes for contributors to Journal of Nanjing Medical University

Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.     

EFFECTS OF ALCOHOL INTAKE ON PENILE STRUCTURE AND FUNCTION IN RATS

Objective To investigate the effects of alcohol intake on penile structure and function in rats. Methods Thirty adult male Wistar rats were randomly divided into two groups: control group and alcohol intake group. They were administered with 2 mL of normal saline and 40% alcohol solution respectively through gastric tubes every day. Three months later, the animal model of alcohol intake was evaluated by modified Nayagida's method, and the effects of alcohol on the rats were studied by sexual behavior, the number of apomorphine-induced penile erection, level of testosterone in the sera, and the content of penile smooth muscle. Results The scores of animal model of alcohol intake evaluated by Nayagida's method were 0.66±2.05 in the control group and 9.26±5.50 in the alcohol intake group(P<0.05), which indicated that an animal model of alcohol intake was successfully established. Sexual behavior, the number of apomorphine-induced penile erection, testosterone level in the sera, and the content of penile smooth muscle of the alcohol intake group were all statistically different as compared with the control group(P<0.05). Conclusion Alcohol intake induces sexual dysfunction in rats, which may be due to the decline of testosterone level in the sera and decline of penile smooth muscle.     

科学、合理成本控制体系的建立促进消毒供应中心发展

目的：探讨消毒供应中心成本控制体系建立,强化消毒供应中心成本管理职能,使无菌物品科学、合理使用,减少无效劳动及物品浪费,促进消毒供应中心发展。方法：用每个重复使用器械成本除以平均使用次数得出每一次使用成本;根据物品清洗、包装、灭菌设备成本除以使用年限及次数得出每一次使用成本;加上一次性消耗人力、物力成本得出每一种无菌物品的成本。结果：消毒供应中心实现成本集中管理,成本直接和使用科室挂钩,各种物品浪费、丢失、损耗大大降低,医院成本得到控制;消毒、灭菌物品实行医院内部收费,工作量与工作人员收入挂钩,工作人员积极性、满意度明显提高。结论：无菌物品成本控制体系的建立规范了消毒、灭菌物品的管理;集中管理提高了医疗质量安全,降低医院成本投入;消毒供应中心集中化、专业化、规范化、工作价值得到明显提升,消毒供应中心各方面管理进入良性发展。     

进展期生殖器和生殖器外硬化性苔藓的临床病理分析

目的 探讨进展期生殖器和生殖器外硬化性苔藓(LS)的临床病理特征并比较其差异。方法 回顾性分析北京医院皮肤科活检确诊的55例进展期生殖器和生殖器外LS患者的临床病理资料并比较两者差异。结果 男11例、女44例;单发于生殖器者38例,单发于生殖器外者15例,生殖器和生殖器外LS并存者2例。生殖器外LS主要及背部(14.55%)和四肢(7.27%)。无症状、瘙痒、疼痛的患者分别占28.30%、73.58%、24.53%,无症状的生殖器外LS患者比例明显高于生殖器LS(χ²=31.224,P=0.000),生殖器LS出现瘙痒的比例明显高于生殖器外LS(χ²=39.073,P=0.000),疼痛感仅见于生殖器LS中。57例活检标本取自上述患者,其中生殖器部位40例,生殖器外部位17例。除真皮浅层均质化外(100%),常见病理改变有角化过度(98.25%),棘层萎缩(50.88%),基底层液化变性(52.63%),血管扩张(64.91%),血管周围淋巴细胞(100%)、组织细胞(85.96%)浸润,苔藓样炎症浸润(54.39%)。生殖器LS标本出现棘层肥厚(χ²=4.402,P=0.036)、血管扩张(χ²=9.330,P=0.002)、嗜酸性粒细胞浸润(χ²=4.162,P=0.041)的比例高于生殖器外LS,出现毛囊角栓的比例低于生殖器外LS(χ²=5.076,P=0.024)。结论 进展期生殖器LS与生殖器外LS之间在临床症状及毛囊角栓、棘层肥厚、血管扩张和嗜酸性粒细胞浸润等病理改变中均有一定差异。     

吻合血管神经足展肌游离移植治疗晚期面瘫的远期疗效评价

目的:评价吻合血管神经足罡母展肌游离移植治疗晚期面瘫的远期疗效.方法:对1990年3月至2006年11月接受该术式的患者共28例进行术后随访1～16年,中位随访时间4年,分析患者手术前后标准头正位照片,采用多伦多评价系统(T-FGS)和面神经功能指数(FNFI)评价面神经功能,并对移植肌存活状况、面宽、瘢痕、供足功能等情况加以分析比较.结呆:所有患者术后均获得理想的静态对称.随访发现所有患者均有移植肌的随意收缩运动,其中22例获理想的微笑.与术前相比,患者术后T-FGS得分提高了(27 7.2)分,FNFI值提高了(57±10.7)%,差异有统计学意义(P＜0.05);术后患者瘢痕隐蔽,供足无遗留功能障碍.结论:吻合血管神经足罡母展肌游离移植治疗晚期面瘫术后远期效果理想,供区无任何功能障碍,值得推广.     

Evaluation of established rheumatoid arthritis

There are two major aspects to evaluate in patients with established RA, namely (i) the state or progress of the disease, and (ii) the effects of specific interventions. The evaluation should include reliable, valid and sensitive measures of disease activity, tissue damage and health status. It is important to recognise that measures of disease activity can be influenced by tissue damage as well. Also, in established RA, health status is likely to be influenced by both disease activity and tissue damage. Whether current rules concerning improvement or remission, which were developed in the context of early RA, can be applied to established disease has to be investigated. While use of radiographs is the current standard method of assessing damage in established RA, range-of-motion measures and muscle strength indices may be a more practical and an equally valid alternative. When selecting health status instruments one should carefully explore and pretest possible health status measures for specific clinical or study settings.     

Preparing for Disaster: Response Matrices in the USA and UK

Disasters, whether man-made or naturally occurring, require complex responses across multiple government agencies and private sector elements, including the media. These factors mandate that, for effective disaster management and because of the unpredictability of such events, response structures must be in place in advance, ready to be activated on short notice, with lines of responsibility clearly delineated and mechanisms for coordination of efforts already established. Disaster response experiences in the USA and the UK were reviewed at a conference convened by the New York Academy of Medicine and the Royal Society of Medicine in June 2007. Lessons to be drawn from these comparisons were sought. The importance of careful advance planning, clear delineation of spheres of responsibility and response roles, effective mechanisms for communication at all levels, and provision for adequate communication with the public were all identified as key elements of effective response mechanisms.