Dermatophyte infections in Melbourne: Trends from 1961/64 to 2008/09

Background/Objective: To characterise the trends in dermatophyte isolation from specimens sourced from metropolitan Melbourne over a 48‐year time period. Method: Analysis of submitted specimens from three time periods. Results: Trends identified include: an increasing mean age of the patients; increased numbers of onychomycosis isolates; a significant age difference among patients with Trichophyton interdigitale isolates compared with patients who had other dermatophyte species; and an increasing number of previously rarely isolated imported dermatophytes. Conclusions: Significant shifts are occurring in dermatophyte epidemiology.     

常见皮肤癣菌的基因分型

目的建立快速、简便鉴别4种常见皮肤癣菌（红色毛癣菌、须癣毛癣菌、絮状表皮癣菌和石膏样小孢子菌）的分子生物学基因分型方法。方法将我室分离培养得到的18株红色毛癣菌、12株须癣毛癣菌、8株絮状表皮癣菌和6株石膏样小孢子菌接种于PDA培养基上培养2周后,分别收集菌丝体并用氯化苄法提取DNA,运用随机扩增多态性DNA分析的方法,以单个引物（GACA）4进行随机扩增。检测聚合酶链反应产物,并根据电泳后指纹图谱的差异进行种属间鉴别。结果同种不同株的皮肤癣菌电泳后指纹图谱相同,不同种皮肤癣菌电泳后指纹图谱有显著差异。结论运用随机扩增多态性DNA分析的方法,可对红色毛癣菌、须癣毛癣菌、絮状表皮癣菌和石膏样小孢子菌的基因型进行快速鉴别,能够协助临床早期诊断。