Increased Level of Soluble E-Selectin in the Serum from Patients with Idiopathic Pulmonary Fibrosis

To elucidate the biological significance of selectin for idiopathic pulmonary fibrosis, we titrated the serum soluble E-selectin. From 31 cases of idiopathic pulmonary fibrosis patients without signs or symptoms of infection, the serum was obtained and the concentration was titrated by enzyme-linked immunosorbent assay. The serum soluble E-selectin titer was significantly higher than that of healthy controls. However, significant elevation was not observed in the sera from the patients with other pulmonary diseases, such as pulmonary emphysema, sarcoidosis, or bronchiectasis. In the patients with idiopathic pulmonary fibrosis, the number of white blood cells, C-reactive protein or lactate dehydrogenase activity did not show a significant relationship with the soluble E-selectin titer. About 16 out of the 31 idiopathic fibrosis patients, the serum surfactant apoprotein-A titer, which is a parameter of the disease activity of idiopathic pulmonary fibrosis, was also tested. The surfactant apoprotein-A titer was loosely correlated with the soluble E-selectin titer. These observations suggest that E-selectin may be relevant to the pathogenesis of idiopathic pulmonary fibrosis, and it may be a novel clinical parameter for idiopathic pulmonary fibrosis.     

中国北方汉族人群E-选择素第2外显子+G98T基因多态性与冠心病关系的研究

目的研究E-选择素(E-selectin)第2外显子+G98T基因多态性与中国北方汉族人群冠心病发病的关系。方法应用聚合酶链反应限制性片段长度多态性(PCR-RFLP)技术检测中国北方汉族无血缘关系的176名冠心病患者和152名健康对照者E-选择素基因第2外显子+G98T基因型;生化技术测定血脂水平;酶联免疫吸附试验(ELISA)双抗体夹心法检测血清E-选择素水平。结果 E-选择素+G98T基因型在冠心病组及对照组中的分布差异有显著性(χ2=4.628,P=0.031),GT基因型者患冠心病的风险是GG基因型的2.221倍(OR=2.221,95%CI=1.001～4.595);T等位基因高于G等位基因,差异具有显著性(χ2=4.351,P=0.037,OR=2.124,95%CI=1.047～4.310);中国北方汉族人群E-选择素基因+98位点T等位基因携带频率明显低于美国、德国人群(P<0.05)。结论 E-选择素基因第2外显子+G98T多态性与冠心病的发病具有相关性,T等位基因可能是北方汉族人冠心病发病的遗传易感基因,E-选择素基因+G98T引起的冠心病发病在一定程度上存在地域和种族差异。     

Kinetics of circulating selectin levels during bone marrow aplasia

Abstract: We have studied the kinetics of plasma levels of circulating (c)selectins in 8 patients undergoing bone marrow or stem cell transplantation to gain estimates for the distribution and half-life of (c)selectins and to potentially identify an endothelial source of cp -selectin in patients who are deprived of platelets and megakaryocytes. Blood was sampled just before conditioning treatment and immediately after, at 2 occasions under bone marrow aplasia (1 and 2 wk after BMT), on 2 separate days (3–8 d apart) before and at 60 min after platelet transfusion, and 30–72 d after BMT. All (c)selectins showed a strikingly parallel decrease during bone marrow aplasia: cp-selectin decreased by a median of 70% (range: 59–95%), CE-selectin by 63% (range: 27–78%), and cl -selectin by 75% (range: 66–90%) compared to baseline (p = 0.012 for all comparisons). Estimates for plasma half-lives of all (c)selectins were obtained from individual time vs. concentration profiles of the maximal decreases and ranged from 2 to 4 d. cp-selectin increased by 23% (p = 0.003), ce -selectin by 5% (p = 0.041) and cl -selectin by 19% (p = 0.009) 1 h after the platelet transfusions. Based on these results the calculated volume of distribution (Vd) of transfused (c)selectins was 2.5-fold higher than the plasma volume, which supports the concept of the in-vivo expression of binding sites, i.e. ligands, for CE-selectin and CL-selectin. In summary, while our study cannot provide any evidence for endothelial cells as a source of cp-selectin, we have found for the first time evidence for the existence of ligands for soluble CE-selectin and cL-selectin in humans.     

电针大鼠足三里穴对胃缺血再灌注过程中胃组织E选择素表达的影响

目的:通过电针刺激胃缺血再灌注大鼠足三里穴,观察电针对缺血再灌注状态胃组织中E选择素表达的影响。方法:采用正常组、胃缺血再灌注组(对照组)和电针刺激病理模型组,使用免疫组化方法分别观察电针足三里穴前后3组胃组织中E选择素的表达。结果:(1)对照组大鼠胃组织,E选择素表达面积较正常组大鼠的有显著升高(P<0.01);(2)电针组大鼠胃组织,E选择素表达面积较对照组的均有所降低,但是没有统计学差异;(3)电针组胃组织,E选择素表达面积与正常组比较有显著差异(P<0.01)。结论:电针刺激足三里穴在胃缺血再灌注过程中对胃黏膜组织的微血管和毛细血管内皮细胞的E选择素表达有减少的趋势。     

黄芪注射液对内皮细胞的增殖及表达 E-Selectin 和 VCAM-1 的影响

目的:了解黄芪注射液对内皮细胞增殖及分泌黏附分子 VCAM-1 和 E-selectin 的影响,探讨黄芪注 射液对造血调控的机理。 方法: (1). 建立人脐静脉内皮细胞体外培养模型,用免疫细胞化学方法及电子显微镜 技术鉴定内皮细胞;(2). 将 HUVEC 与黄芪注射液不同剂量(0滋g / mL、4滋g / mL、40滋g / mL、400滋g / mL 和 4000滋g / mL)一起培养,用 MTT 法检测内皮细胞增殖;以流式细胞分析术测定内皮细胞增殖周期;以 TNF 作为阳性对照, 用 ELISA 法对黏附分子 VCAM-1 和 E-selectin 进行测定。 结果: (1)成功的建立了人脐静脉内皮细胞的体外模 型;用峪因子相关抗原免疫细胞化学染色结果为阳性、电镜检测到 W-P 小体,证实培养细胞为人脐静脉内皮细 胞。 (2)内皮细胞随着培养时间的延长,各组均显示出不同程度的促生长效应(Time:P<0. 05;Concentration:P< 0. 05)。 (3)细胞周期检测显示实验组内皮细胞周期各时相的细胞数占细胞总数的百分比与黄芪组比较有明显 的不同(P<0. 05)。 S 期和 G 2 / M 期细胞显著增多(P<0. 05),而 G 0 / G 1 期细胞相对比例减少(P<0. 05)。 (4) 体外培养的内皮细胞可自然分泌 VCAM-1。 各个浓度的黄芪注射液与 TNF 一样均具有促进内皮细胞分泌 VCAM-1 的作用,其中400滋g / mL 的黄芪注射液浓度是促进内皮细胞分泌 VCAM-1 的最佳浓度(P<0. 05),而且 黄芪注射液具有协同 TNF 的作用(P<0. 05)。 (5)内皮细胞体外培养在没有任何刺激的情况下未检测到 E- selectin. 黄芪注射液刺激内皮细胞也未检测到 E-selectin. 内皮细胞在 TNF 刺激活化后表达 E-selectin. 黄芪注射 液能促进 TNF 活化的内皮细胞分泌 E-selectin(P<0. 05)。 结论: 本研究提示,黄芪的补气生血途径之一可能是 通过直接刺激内皮细胞增殖和分泌造血生长因子和黏附分子,从而间接发挥造血调控作用的;黄芪可能促进造 血干祖细胞的归巢,并为开发黄芪用于辅助治疗血液病及肿瘤放、化疗后骨髓重建提供了理论依据。