Plasminogen activator activity in human endometrial carcinoma

Plasminogen activator activity (PAA) has been proposed as an indicator of aggressiveness of tumors and has been shown to be hormonally modulated. To test this hypotheses in human endometrial carcinoma, tumor explants were assayed for PAA secretion and the results correlated with histopathologic criteria of aggressiveness. The effect of medroxyprogesterone acetate (MPA) on PAA secretion was also investigated. Lower levels of PAA were found to be associated with tumors displaying aggressive tendencies. MPA generally decreased PAA secretion. No apparent correlation was found between estrogen or progesterone receptor concentrations and PAA levels.     

老年男性血清睾酮水平与骨量及骨代谢的关系

目的探讨老年男性血清睾酮（TT）水平与骨质疏松（OP）及骨代谢的关系.方法选取53名健康老年男性.使用DXEA骨密度检测仪测量腰椎（L2～4）、股骨上端（Femneck、Trock、Wards）骨量,并分别计算腰椎、股骨T值,分成腰椎、股骨、腰椎-股骨综合评判组,以T≤-2.0 SD为标准分成OP和非骨质疏松（NOP）两组.用ELLES放免法检测TT、雌二醇（E2）;以TT 13 nmol/L为标准,分为TT正常组和TT不足组.用试剂盒测定血清骨钙素（BPG）,尿1型胶原纤维N端多肽（NTX）及C端多肽（CLP）.应用SPSS10.0软件进行分析.结果（1）股骨组OP与NOP之间TT比较有统计学意义（P＜0.05）,其余各组OP与NOP之间TT、E2水平未见明显差异.（2）TT正常组与TT不足组在血清BPG、尿CLP、股骨T值及Femneck、Trock、Wards部位骨密度值比较均有统计学意义（P＜0.05）.结论老年男性TT水平绝对或相对不足可能通过抑制骨合成、增强骨吸收等多方面使骨量逐渐丢失,这种影响似乎对股骨更明显.     

两套视觉电生理检查系统的比较

目的:对比国特GT-2000NV与RETIscan两套视觉电生理检查系统对正常青年人检查的结果。方法:20例正常军校学员用国特和RETIscan两套系统进行图形视觉诱发电位(PVEP)、视网膜电图(ERG)、多焦视网膜电图(mERG)的检测,两套系统的各相应参数调整至相同或相近。结果:两套系统所检测各结果中除Max-ERGa波幅值外,PVEP和常规ERG的其余各值及mERG在两套系统中差异均有显著性意义。PVEPP100峰时、Rod-ERGa波幅值、Rod-ERGb波峰时、Cone-ERGb波峰时在两套系统的测量中,在α=0.05水准上无相关性,而PVEPP100幅值和常规ERG其余结果在两套系统中均表现为正相关,其中Rod-ERGb波幅值、Max-ERGb波幅值、峰时、Cone-ERGb波幅值、Flick-ERGP2波幅值的相关系数分别为0.679,0.832,0.702,0.756,0.766。mERG环形p波功率密度及各象限p波功率密度及峰时在两套系统中均为正相关,环形p波峰时在α=0.05水准上第1,2,3环无相关性,而第4、5环在α=0.05水准上为正相关。其中环形第1,2,3,4,5环功率密度的相关系数分别为0.443,0.627,0.591,0.592,0.739,其峰时的相关系数分别为0.292,0.269,0.302,0.432,0.810。两套系统在mERG测量中,p波功率密度及峰时的变化具有相同的趋势。结论:国特和RETIscan两套系统在视觉电生理的测量中,其各结果的正常值     

Analysis of Parameters of a Rectified Tank on the Basis of In-Situ Tests

The vertical deflection of building structures is a common problem. However, the rectification of objects is rarely carried out due to the lack of information about the parameters of objects requiring rectification. The subject of the analysis are parameters of rectified water tank 950 m³ in volume, which were investigated due to the stiffness and number of supports built into the structure. During in-situ testing, the stiffnesses of supports were determined. The model of the rectified structure was then defined and it was shown that its parameters can be described by means of three matrices: stiffness, displacement forms of the elevated object and displacement forms of supports. Absolute values of elements of the stiffness matrix increased as the stiffness and number of supports increased. At the same time, values of elements of the matrix of displacement forms of the elevated object increased. The conducted energy analysis demonstrated that the amount of energy required for the vertical displacement of the structure decreased with an increasing stiffness and number of supports. This means that placing a greater number of supports under rectified structures and ensuring more rigid supports is beneficial to the rectification. Results of the conducted analyses were confirmed during in-situ tests.     

Serial changes of prefrontal lobe growth in the patients with benign childhood epilepsy with centrotemporal spikes presenting with cognitive impairments/behavioral problems

Several studies have reported a higher incidence of learning and behavioral difficulties in association with frontal lobe dysfunctions in children with benign childhood epilepsy with centrotemporal spikes (BCECTS). We studied serial changes in frontal and prefrontal lobe volumes using three-dimensional magnetic resonance imaging in BCECTS with or without cognitive impairments and behavioral problems and evaluated correlations between prefrontal lobe growth and active seizure period. Serial changes in regional cerebral volumes were measured in two patients with cognitive impairments and behavioral problems (BCECTS(+)) and five patients without neuropsychiatric deficits (BCECTS(−)). Eleven normal subjects (4-13 years old) served as controls. Volumes of the frontal and prefrontal lobes were determined using a workstation, and the prefrontal-to-frontal lobe volume ratio was calculated. Frontal and prefrontal lobe volumes revealed growth disturbance in BCECTS(+) compared with BCECTS(−) and control subjects. In addition, prefrontal-to-frontal lobe volume ratio increased serially in BCECTS(−) similarly to controls, but was stagnant or decreased in BCECTS(+). Prefrontal growth also revealed more rapid recovery in a BCECTS(+) patient with shorter active seizure period. These findings suggest that longer active seizure period as frequent spike-waves coupled with the occurrence of frequent seizures may be associated with prefrontal lobe growth disturbance, which relates to neuropsychological problems.     

口服克洛已新治疗小儿呼吸道感染疗效观察

目的:探讨口服克洛已新干混悬剂的临床疗效。方法:将76例(男30人,女46人)年龄6月～5岁的急性上呼吸道感染、急性气管炎的惠儿作为研究对象,随机分成两组,治疗组40人,对照组36人,症状均以发热为主要表现、咳嗽轻,肺部均有痰鸣音。两组血常规及cRp均提示细菌感染。两组症状体征无统计学差异。治疗组给予克洛已新干混悬剂(江苏正大清江药业有限公司),其组分为:每袋含头孢克洛250mg,盐酸溴已新8.77mg,按体重以头孢克洛计一日20～40mg/kg,分3次服用,连服5～7天,对照组给予头孢氨苄颗粒和其他化痰药5～7天。疗效判断:显效:服药2天热退4天咳嗽止,肺部痰鸣音消失。有效:服药3天热退6天咳嗽止,肺部痰鸣音消失,无效:7天后仍咳嗽、肺部痰鸣音存在。结果:治疗组总有效率88.7%,对照组65.2%,两组经治疗总有效率差异有统计学意义(P<0.05)结论:克洛已新干混悬剂组方合理,治疗小儿急性上呼吸道感染、急性气管炎疗效显著,且快捷、方便、节省费用,适合在儿科推广。     

子宫肌瘤致病相关基因的筛选与克隆

【目的】 研究子宫肌瘤与正常子宫肌组织中的基因表达差异,筛选及克隆子宫肌瘤的致病相关基因。【方法】 应用荧光标记的mRNA差异显示技术,比较11例子宫肌瘤及其正常子宫肌组织基因表达的差异,对获得差异片段进行克隆、测序及同源性分析,并对其中5条差异片段在子宫肌瘤及正常子宫肌组织中的表达情况进行RT-PCR分析。【结果】 差异片段NO.3,5,11,12在子宫肌瘤正常及异常组织中的表达均存在差异。其中NO.3差异片段在8例子宫肌瘤组织中有表达,而相应的正常组织中仅2例有表达。NO.5,11,12差异片段在子宫肌瘤中的表达水平高于正常子宫肌组织中的表达水平。【结论】 在子宫肌瘤的发生和发展过程中存在多个基因的表达异常,其中ulap8基因有可能在子宫肌瘤组织中呈特异性的表达,ulap14,ulap25,ulap26基因在子宫肌瘤组织中存在表达差异,推测这些基因有可能与子宫肌瘤的发病有关。     

大鼠代谢型谷氨酸受体第5亚型基因片段的克隆

从大鼠的尾壳核组织中提取总 RNA,以 RT-PCR方法扩增出大鼠 m Glu R5 长度约 43 5 bp的 c DNA片段。将这一片段克隆到 PGEM-T载体中进行序列分析 ,结果证实所克隆的 c DNA是编码正确的大鼠 m Glu R5 的一段基因序列。克隆的这段大鼠m Glu R5 特异性基因片段可用于制作探针 ,利用原位杂交技术检测其 m RNA在正常或异常状况下的表达 ;也可制作反意 c DNA或反意 m RNA以研究 m Glu R5 在生理或病理状态下的作用 ;还可进行反意 c DNA或反意 m RNA基因治疗。总而言之 ,克隆的这段基因 ,对研究 m Glu R5 在生理及病理条件下的功能变化 ,以及对与其有关疾病的基础研究和临床应用具有重要意义     

Efficacy of Opiate Maintenance Therapy and Adjunctive Interventions for Opioid Dependence with Comorbid Cocaine Use Disorders: A Systematic Review and Meta-Analysis of Controlled Clinical Trials

Aims: To determine the efficacy of Opiate Maintenance Therapy (OMT) and adjunctive interventions for dual heroin and cocaine dependence by means of a meta-analysis. Method: We searched for and retrieved randomized controlled clinical trials. We used RevMan 5.0 with random effects modeling for statistical analysis and for comparisons of relative risk, effect sizes, and confidence intervals. Subsequent moderator variables and sensitivity analyses were performed. Results: Thirty-seven studies, which have enrolled 3,029 patients, have been included in this meta-analysis. High doses of OMT were more efficacious than lower ones in the achievement of sustained heroin abstinence (RR = 2.24 [1.54, 3.24], p < .0001) but had no effect on cocaine abstinence. At equivalent doses, methadone was more efficacious than buprenorphine on cocaine abstinence (RR = 1.63 [1.20, 2.22], p = .002) and also appeared to be superior on heroin abstinence (RR = 1.39 [1.00, 1.93], p = .05). Several pharmacological and psychological potentiation strategies have been investigated. An improvement on sustained cocaine abstinence was achieved with indirect dopaminergic agonists (RR = 1.44 [1.05, 1.98], p = .03) and with contingency management (CM) focusing on cocaine abstinence (RR = 3.11 [1.80, 5.35], p < .0001). Conclusions: Dual opioid and cocaine dependence can be effectively treated with OMT in combination with adjunctive interventions. Higher OMT doses are preferable to lower ones and methadone to buprenorphine. OMT can be enhanced with indirect dopaminergic drugs and with CM focusing on cocaine abstinence.     

Ⅰ型糖尿病人来源胰岛细胞抗原2特异性T细胞克隆的建立及其细胞毒性研究

目的:为了研究I型糖尿病中细胞介导的自身免疫损伤因子。方法:采用有限稀释法建立糖尿病病人自身抗原IA2抗原特异性的T细胞克隆,51Cr释放细胞毒性试验测定T细胞激活的HLA限制性和IA2肽段中最小的抗原表位,并用流式细胞仪分析其表面凋亡相关的肿瘤坏死因子（Tumor Necrosis Factor,TNF）家族细胞因子Fas配体（Fas Ligand,FasL）、TNF凋亡诱导配体（Tumor　Necrosis　Factor　Apoptosis-Inducing　Ligand,TRAIL）、TNFα表达和细胞内的Perforin和Granzyme　B的表达。结果:建立了IA2抗原特异性的T细胞克隆JDM IA2A2,其为HLA DR5限制,IA2最小抗原表位在IA2（838-846）,其表面没有 FasL表达,39.3％细胞表达TRAIL,29.23％细胞表达TNFα,细胞内没有Perforin,但59.16％细胞有Granzyme B。结论:TRAIL、TNFα、GranzymeB可能参与T细胞介导的胰岛β细胞损伤。