薯蓣皂苷元对人胃低分化粘液腺癌细胞作用的研究

目的观察薯蓣皂苷元(Diosgenin,Dio)对人胃低分化粘液腺癌(MGC-803)细胞的作用,并初步探讨其作用机制.方法MTT法测Dio对MGC-803细胞的抑制作用;考察Dio对MGC-803细胞分裂和集落形成能力的影响;采用3H-TdR掺入法检测Dio对MGC-803细胞DNA合成的影响.结果一定浓度范围内Dio对MGC-803细胞的增殖有剂量、时间依赖性抑制作用;Dio明显抑制MGC-803细胞分裂和集落形成,半数抑制浓度(IC50)为13.17 mg/L,Dio能显著抑制MGC-803细胞DNA合成.结论Dio对MGC-803细胞的抑制作用与抑制该细胞DNA合成有关.     

The kinetics of colony formation by CFU-GM in vitro

Summary. Video-recordings of whole normal bone marrow granulocyte-macrophage colony (CFU-GM) cultures were made after 7, 14 and 21 d. Retrospective viewing of the tapes allowed the relationships to each other of the colonies scored on the three different occasions to be documented. The results show that, according to our scoring criteria, there is very little overlap between the numbers of colonies scored on days 7. 14 and 21. Moreover. only about 10% of the progenitors in a sample form day 21 colonies. The remaining progenitors form colonies earlier in the culture period and either disappear before day 21 or remain small.     

维胺酸对小鼠前胃癌细胞恶性表型的逆转作用及其机制的研究

采用较其母体维甲酸低毒的维胺酸（ＲⅡ）诱导小鼠前胃癌（ＭＦＣ）细胞系分化和细胞恶性表型逆转并分析其可能的机制。实验用１０~（－５）ｍｏｌ／ＬＲⅡ　且连续用药５代结果表明，经ＲⅡ处理后瘤细胞生长受到明显抑制，~３Ｈ－ＴｄＲ的掺入率显著降低，几乎丧失在软琼脂中形成集落的能力。流式细胞仪检测显示，瘤细胞由Ｇ１期向Ｓ期移行受阻断。此外，药物处理后的前胃癌细胞染色体主流范围下降，并开始出现二倍体细胞，且其形态发生了显著变化。培养中的细胞由上皮细胞型转变为成纤维细胞型。组织学观察发现，瘤细胞密度降低，可见到胞浆丰富区，局部瘤细胞出现极性；扫描电镜观察到ＲⅡ处理后的ＭＦＣ细胞微绒毛丰富，丝状伪足的末端常见圆形膨大结构，而对照细胞表面多为折皱状结构，尚可见多量叶状伪足，但未见末端膨大结构。斑点杂交验证ＲⅡ能降低小鼠ＭＦＣ细胞ｃ－ｍｙｃ癌基因的表达，诱导ｖ－ｆｏｓ癌基因的高表达。