Antiulcer activity of Tinospora cordifolia Miers and Centella asiatica linn extracts

The ethanol extracts of the roots of T. cordifolia Miers and C. asiatica Linn were observed to induce a marked protective action against an 8 h restraint stress induced ulcerization, the activity being comparable to that of diazepam.     

HPLC法测定马先蒿属三种药用植物中桃叶珊瑚苷的含量

目的 建立马先蒿属3种植物中桃叶珊瑚苷的测定方法。方法 药材用乙醚脱脂后，甲醇提取，Varian C18柱；流动相：甲醇-水(15：85)；检测波长：204nm；流速：0．5mL／min。结果 桃叶珊瑚苷在太白参、扭盔马先蒿、短茎马先蒿中的含量分别为0．90％，1．94％，0．95％；回收率分别为102．3％，98．2％，97．6％。结论 马先蒿属植物具有较好的药用开发前景，并为其质量评价提供参考。     

真菌诱导子对胡桐悬浮培养细胞产生红厚壳素的影响

目的探讨真菌诱导子对胡桐悬浮培养细胞产生红厚壳素的影响。方法通过对胡桐叶斑病病菌的分离培养,制成真菌诱导子补加到胡桐细胞悬浮培养基中,考察不同浓度、不同加入时间对胡桐细胞生物量及红厚壳素产量的影响。结果S-I菌株诱导胡桐CR2细胞合成红厚壳素的最佳浓度为60 mg GE·L^-1,诱导子在细胞生长静止期初期(即培养d 18)加入对红厚壳素产量影响最大,可使产量提高27%,并促进了红厚壳素向胞外分泌。结论 壳多孢菌的添加能有效提高胡桐细胞悬浮培养体系中红厚壳素的产量。     

中国宽叶缬草药用功效研究概况

从宽叶缬草的镇静活性、抗抑郁活性、调节血脂及抗脂质过氧化作用、对肾脏的保护作用、以及对胆囊结石和心、脑血管系统疾病的治疗作用等七个方面综述了近三年来国内对宽叶缬草药用功效的研究进展。     

Antioxidative Potential of Duranta Repens (Linn.) Fruits Against H2O2 Induced Cell Death In Vitro

The effects of Duranta repens fruits were investigated on H₂O₂ induced oxidative cell death to evaluate its antioxidative potential in vitro. HEK293T cells were treated with different concentrations [0–1000 µg/ ml] of ethanol extract (E-Ex) and methanol extract (M-Ex) of D. repens for 24h, and then treated with 100 µM H₂O₂ for 24h. Cell viability, antioxidant parameters of cells, and antioxidant constituents of the extracts were determined. Treatment with limited dose of E-Ex or M-Ex increased the survival rate of H₂O₂-treated HEK293T cells, however the extra-high dose showed growth inhibitory effect. Treatment with E-Ex or M-Ex protected cellular lipid per-oxidation. In vitro analyses showed the 2,2-diphenyl-1-picrylhydrazyl and H₂O₂ scavenging activities as well as reducing potential of the extracts. We report here that the limited dose of E-Ex and M-Ex possess antioxidative potential, which can protect H₂O₂-induced oxidative cell damage.     

天茄子不同提取部位止血作用研究

目的研究天茄子不同提取部位的止血药理作用。方法依次以环己烷（T1）、水（T2）和乙醇（T3）为溶剂进行提取并对乙醇提取物依次用石油醚（T4）、乙酸乙酯（T5）、正丁醇（T6）进行萃取得到相应的部位以及水部位（T7）,采用毛细玻管法、玻片法、剪尾法和测定血浆复钙时间检测其止血作用。结果 T3、T6、T7可缩短小鼠玻片法凝血时间,T3、T5、T6、T7可显著缩短小鼠毛细管法凝血时间,T3、T4、T5、T6、T7可显著缩短小鼠断尾法出血时间,T3、T6、T7可显著缩短小鼠血浆复钙时间。结论天茄子95%醇总提物、乙酸乙酯部位、正丁醇部位、水部位凝血效果显著,其中95%醇总提物、正丁醇部位、水部位凝血效果极其显著。     

Antimalarial activity and toxicity of Garcinia mangostana Linn.

Objective:To investigate the antimalarial activity and toxicity of the crude ethanolic extract of its pericarp both in vitro and in vim.Methods:The antimalarial activity of Gareinja mangostana(G.mangostana)Linn.extract against 3D7 and Kl Plasmodium falciparum(P.falciparum)clone were assessed using SYBR green I-based assay.A 4-day suppressive test of Plasmodium berghei{P.berghei)infected mouse was performed to investigate in vivo antimalarial activity.Results:The in vitro antimalarial activity was seleclive(SI5?and classified as weak and good lo moderate activity against both 3D7 and K1 P.falciparum,clones with median IC_(50)(range)values of 11.12(10.94-11.29)and 7.54(6.80-7.68)μg/mL,respectively.The extract was considered nontoxic to mice.The maximum tolerated doses for acute and subacute toxicity in mice were 5 000and 2 000 mg/kg,respectively.Median(range)parasite density on day 4 of the negative control group(25%Tween-80),mice treated with 250,500,1000,and 2 000 mg/kg body weight of the extract,and 10 mg/kg body weight of chloroquine for 14 d were 12.8(12.2-13.7),11.4(9.49-13.8),11.6(9.9-12.5),11.7(10.6-12.8),10.9(9.4-11.6)and 0(0-0)%respectively.Parasite density on day 4in the control group treated with Tween-80 was higher than the groups treated with chloroquine and all dose levels of the extract.Conclusions:G.mangostana linn,showed weak antimalarial activity of the extract both in vitro and in vivo could be due to limitation of absorption of the active compounds.     

无花果果实中化学成分研究

目的研究桑科榕属植物无花果Ficus carica果实的化学成分。方法综合运用硅胶柱色谱、ODS柱色谱、Sephadex LH-20凝胶柱色谱以及制备型高效液相色谱等技术进行系统分离,根据化合物的理化性质及波谱数据鉴定化合物的化学结构。结果从无花果果实的90%乙醇提取物中分离得到了16个化合物,分别鉴定为怀特酮(1)、甘草宁G(2)、4′-羟基-5,7-二甲氧基-6-(3-甲基-丁烯基)-异黄酮(3)、猫尾草异黄酮(4)、indicanine C(5)、木豆素(6)、美迪紫檀素(7)、高丽槐素(8)、4-羟基-3-甲氧基-8,9-亚甲二氧基紫檀烷(9)、3-羟基-9,10-二甲氧基紫檀烷(10)、松脂素(11)、杜仲树脂酚(12)、丁香树脂醇(13)、8-羟基松脂醇(14)、3-羟基-1-(4-羟基-3-甲氧基苯)-1-丙酮(15)和4-羟基-3-甲氧基苯乙醇(16)。结论所有化合物均为首次从无花果中分离得到。     

迷迭香中2个新松香烷型二萜化合物

目的研究迷迭香Rosmarinus officinalis的化学成分及其活性。方法采用多种柱色谱对迷迭香地上部分甲醇提取物进行分离纯化;通过波谱数据对化合物进行结构鉴定;采用磺酰罗丹明B比色法(SRB法)对所得化合物进行细胞毒活性测试。结果从迷迭香的甲醇提取物中分离得到2个新的松香烷型二萜类化合物和8个已知二萜类化合物,分别鉴定为7-异丙氧基-表异迷迭香酚(1)、7-异丙氧基-迷迭香醌(2)、迷迭香酚(3)、7-甲氧基-迷迭香酚(4)、表迷迭香酚(5)、7-甲氧基-表异迷迭香酚(6)、rosmaquinoneB(7)、鼠尾草酚(8)、galdosol(9)和pisiferal(10)。结论化合物1和2为新化合物,分别命名为7-异丙氧基-表异迷迭香酚和7-异丙氧基-迷迭香醌。活性测试结果表明所有二萜类化合物均无明显细胞毒活性。     

苎叶蒟中1个新的含氮木脂素

目的对苎叶蒟Piper boehmeriifolium的枝叶部位进行化学成分研究。方法采用硅胶、RP-C_(18)柱色谱、Sephadex LH-20凝胶柱色谱和半制备HPLC等方法进行分离和纯化,并综合运用IR、HR-ESI-MS、~1H-NMR、~(13)C-NMR、DEPT、HSQC、HMBC等方法鉴定化合物的结构。结果从苎叶蒟乙醇提取物中分离得到15个化合物,分别鉴定为3-(3,4-methylenedioxybenzyl)-4-(3,4-dimethoxybenzyl)-1H-pyrrole-2,5-dione(1)、2E,4E-decadienoylpiperidide(2)、2E-decenoylpiperidide(3)、墙草碱(4)、piperchabamide B(5)、假蒟亭碱(6)、piperdardine(7)、胡椒新碱(8)、胡椒碱(9)、二氢荜茇明宁碱(10)、chingchengenamide A(11)、假荜茇酰胺B(12)、guineensine(13)、荜茇宁(14)和kusunokinin(15)。结论化合物1为新化合物,命名为胡椒木脂酰亚胺,化合物2、3、7、8、10和15为首次从该植物中分离得到。