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目的:研究乌司他丁对50%总体表面积烫伤大鼠炎症介质、肺微血管通透性和肺组织含水率的影响。方法:40只雄性SD大鼠随机分为烫伤组和乌司他丁组(n=20),两组均用80℃水浴造成50%总体表面积Ⅲ度烫伤。于烫伤后即刻静脉分别注射生理盐水1ml和乌司他丁40 000U/kg。分别于烫伤后6h、24h采用伊文斯蓝(EB)法测定肺微血管通透性,干/湿重法测定肺组织含水率,酶联免疫吸附法(ELISA)检测肺组织TNF-α、IL-6和IL-8的活性。结果:烫伤后6h、24h乌司他丁组TNF-α、IL-6和IL-8均低于单纯烫伤组,其中烫伤后24h乌司他丁组TNF-α、IL-6和IL-8的变化差异更显著(P<0.01);与单纯烫伤组比较,乌司他丁组肺组织EB含量和含水率均显著降低,差异均有统计学意义(P<0.05或P<0.01)。结论:乌司他丁能减轻致死性烫伤休克大鼠肺组织炎症水平,抑制血管内皮细胞活化和通透性增加,减少烧伤休克时血容量丢失和补液量,用于烧伤休克的早期治疗和改善存活率有潜在价值。 相似文献
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目的：研究乌司他丁对50％总体表面积烫伤大鼠全身炎症、脏器微血管通透性和组织含水率的影响。方法：32只雄性SD大鼠随机分为烫伤组和乌司他丁组（n=16）,两组动物均造成50％总体表面积Ⅲ度烫伤。于烫伤后即刻静脉分别注射生理盐水或乌司他丁40000U／kg。于烫伤后4h取血浆测定肿瘤坏死因子-α、C反应蛋白和髓过氧化物酶,然后处死动物,采用伊文斯蓝法测定心、肝、肺、肾和小肠微血管通透性,干／湿重法测定组织含水率。结果：烫伤后4h乌司他丁组血浆C反应蛋白含量和髓过氧化物酶活性显著低于烫伤组（P均〈0．01）。乌司他丁组肺、肾和小肠组织伊文斯蓝含量和含水率均显著低于烫伤组（P〈O．05或P〈0．01）。结论：乌司他丁能抑制致死性烫伤休克大鼠血浆促炎症因子水平,减轻组织炎症反应,降低脏器微血管通透性和组织水肿,减少血管内液体丢失,可用于烧伤休克的早期药物治疗。 相似文献
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目的:研究乌司他丁对炎症因子诱导的体外培养的大鼠肺微血管内皮细胞通透性和细胞因子产生的影响.方法:体外培养大鼠肺微血管内皮细胞分组进行实验:①对照组:内皮细胞培养液中加入生理盐水或乌司他丁;②炎症因子刺激组:因培养液中分别含烫伤血清、胰蛋白酶、前列腺素E2(PGE2)、缓激肽、组胺而分为5个炎症因子刺激亚组;③乌司他丁... 相似文献
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Shiyan Li Weixi Yang Liangliang Zhou Dekang Nie Haizhou Yu 《The American journal of emergency medicine》2019,37(2):249-253
Background
This study aimed to investigate the preventive and therapeutic effects of ulinastatin on the recovery of vascular permeability and hemodynamics in shock-affected organs during early burn injury.Methods
Twelve beagle dogs with 35% TBSA full-thickness burn were randomized to control group (CG, n?=?6) and ulinastatin group (UG, n?=?6).Hemodynamic parameters were determined by PICCO before burn and at 0.5?h, 1?h, 2?h, 4?h, 6?h, 8?h post-burn. Plasma volume (PV) were deduced through indocyanine green (ICG) method. Blood samples were drawn to determine hematocrit (HCT) at each time. Intestinal mucosal blood flow (IMBF) was determined by laser Doppler flowmetry. Evans blue (EB) method was taken to measure the vasopermeability of heart, liver, spleen, kidney and small intestine. Pathological tests were also performed.Results
Compared with CG,UG had better hemodynamic parameters (improved mean arterial pressure and cardiac output) (P?<?0.05). HCT rose in both groups, while in UG the rising extent was less than that in CG (P?<?0.05). PV and IMBF in both groups declined, but in UG the extent were less (P?<?0.05). Both EB content of liver, spleen, kidney and small intestine in UG were much lower than that in CG at 8?h post-burn (P?<?0.05). Pathological changes showed that severe injury was significantly ameliorated in UG.Conclusions
Ulinastatin helped to moderate the quick loss of circulatory volume and improved hemodynamic indices in beagle dogs who had suffered 35% third degree burn injuries. It may serve as a therapeutic agent for reducing vascular permeability, thereby preventing fluid loss following major burn injury. 相似文献5.
目的:观察超排卵对小鼠着床的影响和中药帮得孕的改善作用。方法:仿照临床超排卵方案,运用腹腔注射孕马血清促性腺激素(PMSG)10IU,于48h后注射绒毛膜促性腺激素(HCG)10IU的方法造模。观察妊娠第8天正常组、超排卵组、不同剂量帮得孕组小鼠胚泡着床率和平均胚泡着床点数。伊文思蓝染色观察妊娠第5天子宫内膜血管通透性情况,HE染色观察妊娠第5天子宫内膜蜕膜化情况,免疫组化和Western blot方法观察妊娠第5天子宫内膜基质金属蛋白酶-9(MMP-9)的表达。结果:与正常组相比,超排卵组着床率明显下降(P<0.05),与超排卵组相比,帮得孕各剂量组着床率均明显提高(P<0.05);超排卵抑制小鼠子宫内膜血管渗透性,延缓子宫内膜蜕膜化,抑制子宫内膜MMP-9表达,中药帮得孕则有利于促进子宫内膜血管渗透性、促进内膜蜕膜化、促进MMP-9表达。结论:超排卵可致着床障碍,与超排卵药物抑制血管渗透性、抑制子宫内膜蜕膜化和抑制内膜基质降解有关,帮得孕可以一定程度上降低和抵消超排卵这种不良的影响,有利于胚泡成功着床。 相似文献
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