基于自组装单分子层技术的压电石英晶体DNA传感器固定方法的研究

目的 探讨一种高效的压电石英晶体DNA传感器固定的方法。方法 分别采用常规自组装法（未还原探针法）与探针预先经过还原的自组装法（还原探针法），将针对铜绿假单胞菌的探针固定在DNA传感器的金膜表面，比较不同探针浓度下，探针固定和杂交反应所引起的频率的变化以及所用的时间。结果 与未还原探针法相比，还原探针法具有探针固定量大、反应速度快、频率下降明显等优点。结论 还原探针法提高了DNA探针的固定效率，优于未还原探针法。     

压电石英晶体传感器阵列检测尿微量白蛋白的实验研究

目的探讨并建立压电石英晶体免疫传感器检测尿液微量白蛋白的方法。方法采用葡萄球菌A蛋白(SPA)法固定石英晶体微阵列上单克隆抗白蛋白抗体,并用石英晶体微天平(QCM)检测样品反应频率漂移量;优化传感器检测条件并运用回收实验,天内、天间重复试验来验证其准确度与精密度,并将免疫比浊法作为参比方法进行方法学比较。结果该传感器平台的非特异性反应低(FS<20Hz),线性范围广(0.01~40mg/L),灵敏度达到5μg/L,准确度高(CV<10%),精密度试验总体CV<10%,并且具有检测时间短(<10 min)等优点,与传统的免疫比浊法具有可比性。结论采用压电石英晶体免疫传感器法检测尿液微量白蛋白,是一种可行的检测方法,可应用于临床检测并为诊断早期肾损伤提供帮助。     

新型压电石英晶体免疫传感器抗体固定方法的建立

目的探讨并建立一种高效免疫微阵列传感器抗体固定的方法.方法分别采用普通蛋白A(SPA)法与经巯基取代SPA(自组装SPA)法固定石英晶体微阵列上抗体,并用石英晶体微天平(QCM)分别检测其不同浓度抗体、抗原条件下发生抗原抗体反应,并对其抗体包被量、反应频率漂移、非特异性反应等多项指标进行统计学分析.结果与传统SPA法相比,自组装SPA具有抗体固定量大、反应速度快、频率下降明显、非特异性反应低等优点.结论自组装SPA法具有比普通SPA法包被一致性更高的优点,可以作为新一代免疫传感器的抗体固定方法.     

气候因素对青铜文物模拟材料大气腐蚀的影响

运用QCM反应性监测方法研究了暴露在博物馆模拟展柜中的青铜文物模拟材料的腐蚀行为及规律,并利用扫描电镜(SEM)、能谱仪(EDS)以及光电子能谱(XPS)分析手段,对青铜文物模拟材料在腐蚀60 d后的腐蚀形貌与产物进行了分析。结果表明:光照的存在以及温度、湿度的升高都会加速青铜文物模拟材料的腐蚀,其中ERCO LED灯在色温为4 000 K时对青铜文物模拟材料的腐蚀影响最严重,在腐蚀60 d后的主要腐蚀产物为Cu₂O、CuO、SnO、SnO₂,还有少量铜的碳酸盐、铜的硫酸盐与铜的硫化物、铜的硝酸盐与亚硝酸盐、铜的氯化物。     

Effects of Surface Modification on Adsorption Behavior of Cell and Protein on Titanium Surface by Using Quartz Crystal Microbalance System

Primary stability and osseointegration are major challenges in dental implant treatments, where the material surface properties and wettability are critical in the early formation of hard tissue around the implant. In this study, a quartz crystal microbalance (QCM) was used to measure the nanogram level amount of protein and bone marrow cells adhered to the surfaces of titanium (Ti) surface in real time. The effects of ultraviolet (UV) and atmospheric-pressure plasma treatment to impart surface hydrophilicity to the implant surface were evaluated. The surface treatment methods resulted in a marked decrease in the surface carbon (C) content and increase in the oxygen (O) content, along with super hydrophilicity. The results of QCM measurements showed that adhesion of both adhesive proteins and bone marrow cells was enhanced after surface treatment. Although both methods produced implants with good osseointegration behavior and less reactive oxidative species, the samples treated with atmospheric pressure plasma showed the best overall performance and are recommended for clinical use. It was verified that QCM is an effective method for analyzing the initial adhesion process on dental implants.     

Vacuum Ultraviolet Photolysis of Hydrocarbon Polymers

Summary: The wavelength‐dependent vacuum ultraviolet (VUV) photolysis of several polymers, low density polyethylene (LDPE), biaxially oriented poly(propylene) (BOPP), atactic polystyrene (PS), and poly(methyl methacrylate) (PMMA), was studied by irradiation in vacuum with the well‐characterized emissions from four different resonant or excimer VUV sources. These lamps comprise radiofrequency (r.f.) discharges in different noble gases, such as krypton, xenon (at low pressures, producing near‐monochromatic resonant line radiations), xenon excimer (at “high” pressure), and a deuterium/argon mixture (producing a broad‐band emission). VUV‐induced mass loss (ablation or etching) was monitored in situ by quartz crystal microbalance measurements. Following irradiation, samples were analysed by ATR‐FTIR and XPS, to evaluate near‐surface structural changes (e.g., creation of unsaturation, cross‐linking) resulting from the VUV‐initiated bond scissions and radical‐creation reactions. PMMA was the most readily ablatable polymer, whereas the mass loss of BOPP was higher than that of LDPE, regardless of the irradiation wavelength, λ. All polymers were found to form double bonds, with the exception of PS, which is rather stable, probably due to energy dissipation by fluorescence.

Formation of double bonds in a) vinyl‐, b) vinylidene‐, and c) vinylene‐like unsaturated groups, as a function of the radiation dose, D, for KrL (?), XeL (?), and D₂Ar‐irradiated (?) PMMA.     

新型纳米金界面压电石英晶体DNA传感器的实验研究

目的构建一种新型纳米金界面压电石英晶体DNA传感器,探讨纳米金界面上进行寡核苷酸探针固定的最佳条件。方法构建纳米金颗粒修饰的石英晶振生物反应界面,进行巯基修饰的寡核苷酸探针的固定,对纳米金自组装pH值条件、探针固定浓度等影响因素进行实验研究;将构建的传感器和未经纳米金颗粒修饰的传感器用于铜绿假单胞菌检测,比较两种传感器的响应性能,并进一步对该纳米金界面压电石英晶体DNA传感器的再生进行研究。结果纳米金自组装的最佳pH值为6.0,探针最佳固定浓度为3.0μmol/L。纳米金颗粒修饰的石英晶振的响应性能大于未经修饰的石英晶振,可以重复使用5次。结论纳米金界面压电石英晶体DNA传感器,具有不需标记、操作简单、能实时在线检测、频率响应性能高、无污染和能重复使用等优点,在临床实验诊断方面应用前景广阔。     

Growth and electrochemical activity of the poly-l-lysine|poly-l-glutamic acid thin layer films: an EQCM and electrochemical study

Quartz crystal microbalance (QCM) measurement and cyclic voltammetry were used to study the layer-by-layer growth of poly-l-lysine (p-Lys) and poly-l-glutamic acid (p-Glu) thin films and their ability to act as support for redox relays between metal electrodes and redox species in organic electrolytes. The mass of the film grown from buffered solution at constant pH depends on the composition of the buffer used. The growth proceeds in several stages with different mass increments for each poly-l-lysine and poly-l-glutamic acid layer. Ferro/ferricyanide confined into the polypeptide films can play the role of a redox relay between the electrode surface and the organic electrolyte. This was demonstrated on the impregnated polypeptide film|1,2-dichloroethane interface with decamethylferrocene dissolved as redox species in the organic phase. The transport of the ferro/ferricyanide in the polypeptide film is the rate limiting step of the electron transfer process. The diffusion coefficient of the ferro/ferricyanide determined from voltammetric experiments was of the order of 10^?11–10^?12 cm² s^?1. The bimolecular rate constant of the electron transfer reaction was found to be 0.2 cm s^?1 M^?1.     

C6mimCl/QCM传感器对有机酸性气体的监测

采用离子液体氯化1 己基 3 甲基咪唑（C6mimCl）作为石英晶体微天平（QCM）的涂敷材料制成C6mimCl/QCM传感器,对以甲酸和乙酸为代表的有机酸性气体进行监测。研究结果表明,C6mimCl/QCM传感器对甲酸和乙酸气体均具有响应速率快、灵敏度高和重现性好等特点,该传感器的频率响应信号与被测有机酸性气体浓度呈良好的线性关系,可用于污染物浓度不断升高的博物馆环境的实时在线监测。