hPer1相互作用蛋白的筛选

目的 应用酵母双杂交系统筛选血液中与人体生物钟蛋白Per1相互作用的新蛋白.方法 以人Per1的basic HLH-PAS结构域为诱饵,在人血液cDNA文库中筛选与之相互作用的新蛋白.阳性克隆送测序后用生物信息学分析.结果 酵母双杂交文库营养缺陷筛选得到114个阳性克隆,β-半乳糖苷酶检测报告基因获得46个蓝色克隆.通过测序和生物信息学分析,其中之一能与Per1相互作用的蛋白为LSMD1.结论 通过酵母双杂交系统证明Per1能够与LSMD1发生相互作用.     

CPAN统计模块在医学统计中的应用

目的:在商业的统计软件之外寻找免费的专业统计方法。方法:对CPAN网站进行搜索,寻找并测试其中的统计模块。结果:CPAN网站中的统计模块功能强大计算准确,而且易于使用。结论:在医疗卫生统计过程中我们可以利用CPAN中的模块完成统计计算。     

基于动态脚本语言的食管癌IMRT计划设计

目的：利用Solaris 10操作系统中自带的Perl程序创建具有自适应性质的动态脚本,实现调强放射治疗( IMRT)计划设计的自动执行,并研究其在计划设计中的应用前景。方法选取6例食管癌患者,利用Perl程序创建动态脚本,通过脚本回放,实现IMRT放疗计划的设计,并利用体积剂量,计划完成时间等参数与人工设计的计划进行对比。结果在不明显改变计划质量的前提下,脚本计划时间(12．70±1．47)min相比人工计划时间(27．72±2．29) min平均缩短15 min,差异有统计学意义(P<0．05)。结论利用Perl程序创建动态脚本能够自适应靶区与剂量,通过脚本回放可快速完成IMRT计划的设计。     

ON THE ROLE OF RECURRENT INHIBITORY FEEDBACK IN MOTOR CONTROL

This article reviews presumed roles of recurrent inhibition in motor control, that have been proposed over the past five decades. The discussion is structured in an order of increasing complexity. It starts out with the simplest and earliest circuit, that is recurrent self-inhibition of skeleto-motoneurons, and related functions. It soon becomes clear that in order to understand recurrent inhibition, we must look beyond the simple self-inhibitory CNS circuit. First, recurrent inhibition must be seen in the context of other neural circuits. Second, some quantitative features appear to be correlated with features of the neuromusculo-skeletal periphery. Third, the aspect of lateral inhibition between different members of a motoneuron pool as well as between different motoneuron pools points to the essential multiple input-multiple output structure of recurrent inhibition that again can be understood only by correlating it with features of the neuromusculo-skeletal periphery. Another extension results from the discovery that recurrent inhibition affects not only skeleto-motoneurons, but also γ-motoneurons, Ia inhibitory interneurons mediating reciprocal inhibition between antagonist motoneurons, other Renshaw cells and cells of origin of the ventral spinocerebellar tract (VSCT). Then the view broadens again, investigating the potential role that recurrent inhibition plays in two far-ranging theories of motor control, the inverse-dynamics approach and the equilibrium-point hypothesis. Finally, the present author tries to formulate, in broad strokes, a personal functional interpretation of recurrent inhibition. All the functional considerations, right or wrong, should yield ideas for new experiments, and this then is the last objective of this review. Copyright © 1996 Elsevier Science Ltd.     

探讨解决四格表确切概率计算出现大数溢出错误的方法

目的:解决Fisher确切概率计算中大数溢出的问题。方法:尝试使用Perl语言编写计算程序。结果:编写的程序运行结果正确,没有出现溢出问题。结论:通过Perl程序可以较好地实现四格表确切概率计算。