嘉定区1988—1993年细菌性食物中毒样品检测分析

本区六年共检测食物中毒样品３２３份，检出致病菌１３８株，检出率为４２．７２％。致病菌菌型以付溶血性弧菌居首（７６．８１％），溶藻弧菌次之（８．７０％），鲍氏１￣６型痢疾菌最低（０．７２％）。六年来致病菌检出无升降趋势（ｒ＝０．０２８，Ｐ〉０．０５）。致病菌检出第三季度较高；男女间致病检出相差显著（χ＾２＝１２．３５，Ｐ〈０．０１）。     

Toll 样受体4在恶性淋巴瘤中的作用

恶性淋巴瘤的发病机制一直是肿瘤研究的一个重要课题，但至今尚不完全明确。最近有临床研究表明TLR4（Toll－like receptor 4，TLR4）在套细胞淋巴瘤组织中和多种淋巴瘤细胞系中均有较高表达，且与预后有一定相关性。本文对TLR4在恶性淋巴瘤发生发展中的作用做一综述，以阐明淋巴瘤发病机制并为其治疗提供新的依据。     

Clinical and laboratory characteristics in septic arthritis patients with and without isolated germs

Background

The management of septic arthritis without bacteriological evidence is not well codified.

牙胚组织原位杂交方法学探讨

目的探讨牙胚组织原位杂交的最佳方法。方法用牙本质涎磷蛋白DSPP特异性寡核苷酸探针,检测不同时期牙胚DSPPmRNA的表达。结果各种牙胚的成牙本质细胞和前成釉细胞均检测到DSPPmRNA的表达,各阴性对照组均未检测到信号。结论所用的牙胚组织原位杂交方法敏感性高,特异性高。     

尿液标本的病原菌分布及药敏试验

目的：了解尿液标本中的病原菌分布及耐药率。方法：用常规方法进行生化鉴定和药敏试验，根据NCCLS推荐药敏法并结合本院临床要求所选用抗生素。结果：243份尿标本分离出103株病原菌，其中大肠埃希氏菌50株占48．5％，酵母样菌18株，占17．5%。结论：尿中病原菌种类较多，以大肠埃希氏菌为主，其对青霉素类耐药率较高，对复达欣和阿米卡星敏感率较高。     

重症监护病房呼吸机相关性肺炎的病原学分布和临床分析

目的:了解重症监护病房(ICU)呼吸机相关肺炎(VAP)发生率、病原菌分布情况和预防措施。方法:回顾分析我院2002年5月至2006年4月使用呼吸机的115例患者中出现45例VAP患者的临床资料、病原菌分布以及药敏结果。结果:115例机械通气患者中有45例发生了VAP,VAP发生率为39.1%,病死率为33.3%。共分离出病原菌206株,革兰氏阴性杆菌154株占74.8%,革兰氏阳性菌54株占21.4%,真菌8株占8.9%,结论:革兰氏阴性杆菌是重症监护病房呼吸机并发肺部感染的主要致病菌,临床医师可根据药敏试验合理选用抗菌素。     

Distribution pattern of cholinesterase enzymes in human tooth germs

The two distinct molecular forms of cholinesterase (ChE) are acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). Our previous studies have reported that ChE is involved in tooth development. However, further experiments are needed to understand the precise action of ChE in tooth development. This study aimed to localise types of ChE in human tooth germs, and identify their distribution pattern.ChE were localised in frozen sections of jaws which were prepared from dead fetuses, neonates and stillborns who were free from visible abnormalities by Karnovsky and Root method.AChE was identified in the inner and outer enamel epithelia including the cervical loop region, stratum intermedium and preameloblasts of tooth germs at bell stage. Secretory ameloblasts were free from staining. The bud and cap stages of permanent tooth germs showed AChE activity on the lingual aspect and top surface of the epithelial ingrowths, respectively. BuChE activity was localised in the degenerating dental lamina.Our study reported the first evidence of localisation of ChE in human tooth development and identified the possible molecular form of ChE in tooth germs as AChE. Also, our results have provided strong evidence to speculate the action of AChE is on the cells of enamel organ during tooth development.     

呼吸道感染病原菌分布及耐药性调查

目的：了解我院呼吸道感染病原菌谱及耐药性。方法：对我院2006年1月～9月临床送检的痰及咽拭子进行细菌培养,采用美国德灵公司Auto Scan-4微生物分析系统进行细菌鉴定和药物敏感试验;采用ATB Fungus进行真菌药物敏感试验。按NCCLS标准判定敏感菌株。结果：共分离到病原菌119株,革兰阴性杆菌占68.07%,真菌占26.05%,革兰阳性球菌仅占5.88%。革兰阴性杆菌以肺炎克雷伯菌、大肠埃希菌、铜绿假单胞菌为主,分别占病原菌总数的36.13%、7.56%、6.72%;真菌以白色假丝酵母菌为主,占真菌总数的54.84%。药敏结果提示,铜绿假单胞菌、肠杆菌属、沙雷氏菌属等均出现多重高比例耐药,真菌也对唑类药物出现强耐药性。结论：最近呼吸道感染病原菌以革兰阴性杆菌和真菌为主,而且出现多重耐药。     

Root formation by tooth germs following extraction of prematurely erupted upper first premolars. Case report

A case is described of a seven year-old boy in whom root formation continued following the premature extraction of both maxillary first premolars.     

大骨节病病因研究新假说——产生硫酸软骨素CHS酶的厌氧菌是主要病因

在益都、永寿KBD患区水源中分出产CHS酶厌氧菌,并能引致小鼠关节软骨损害的基础上,近一步在新病例较多的天水、汉源作病因调查,并以骺板软骨发育变化为指征研究KBD成因、发现4患区水源均被粪便严重污染。厌氧菌占粪便菌群结构的9.9％,说明其存在的普遍性。厌氧菌进入鼠体18天后引起骺板细胞柱增殖层细胞成倍增长及坏死、消失等病理变化与人类KBD早期病变一致。“改水”去因后即无新病例出现,说明防治有效。综合三方面结果说明厌氧菌是KBD的主要病因。