Bio—Normalizer（BN）对体外鼠胚大脑神经细胞缺氧损伤保护作用的研究

目的：为研究Bio-Normalizer(BN)对大脑神经细胞缺氧损伤的保护作用,并探讨其机制。方法：本研究采用无血清体外细胞培养方法,培养鼠胚大脑神经细胞,将神经细胞造成缺氧损伤,在无血清培养液中加入不同剂量的BN,观察BN对缺氧损伤的保护作用。将细胞分为4组,Ⅰ组为缺氧BN浓度为0mg/ml组;Ⅱ组为缺氧BN浓度为0．1mg/ml组,Ⅲ组为缺氧BN浓度为0．5mg/ml组;Ⅳ组为非缺氧BN浓度为0mg/ml组。在倒置显微镜下观察细胞生长发育的情况,于细胞培养后的第4天收集细胞,测定生化指标,同时进时光镜和电镜病理检查。结果：缺氧0．1mgBN/ml组的神经细胞MTT及NSE活性均高于缺氧0mgBN/ml组,并有显著差别（P＜0．05）;形态学观察结果也显示缺氧0．1mgBN/ml组和0.5mgBN/ml组的神经细胞缺氧损伤后恢复明显好于缺氧0mgBN/ml组。结论：BN对大脑神经细胞缺氧损伤恢复具有明显的促进作用。     

木瓜粉对缺血性脑梗塞患者总抗氧化能力的影响

目的 :观察木瓜粉对缺血性脑梗塞患者总抗氧化能力(TOC)的影响。方法 :以60名年龄在50～70岁的缺血性脑梗塞初发病例为研究对象 ,随机分为病例组和对照组 ,病例组服用木瓜粉 ,对照组服用维生素C ,随访60天 ,比较两组血中总抗氧化能力的变化。结果 :病例组实验末、实验中TOC高于实验前 ,差异有显著性 (P<0.05)。结论 :木瓜粉能够提高缺血性脑梗塞患者的抗氧化能力     

两种湿敷法对动静脉内瘘皮下血肿的疗效对比

目的 探讨木瓜酒和消肿止痛酊湿敷动静脉内瘘皮下血肿的效果.方法 选取2011-01～2013-10间我科血液净化室发生动静脉内瘘皮下血肿的血液透析患者62例,将其分为观察组和对照组,每组各31例.对照组：采用消肿止痛酊湿敷;观察组：使用木瓜酒湿敷.观察两组患者血肿吸收时间、皮肤瘀斑消退时间及血管硬化方面情况.结果 观察组与对照组血肿吸收时间、皮肤瘀斑消退时间无差异性（P＞0.05）,但是血管硬化方面比较,差异有显著性意义（P＜0.01）.结论 对于动静脉内瘘皮下血肿使用木瓜酒及消肿止痛酊湿敷都能促使血肿消退,但木瓜酒对血管弹性修复效果更好,制作简单,适合临床使用.     

Nutritional composition of Rainbow papaya, the first commercialized transgenic fruit crop

Rainbow papaya (Carica papaya L.) is a genetically engineered (GE) cultivar with resistance to papaya ringspot virus (PRSV). This cultivar currently accounts for about 70% of Hawaii's papaya acreage. The nutritional composition of Rainbow papaya and a non-transgenic control were analyzed to address GE food safety concerns regarding the potential for altered nutritional composition and altered expression of inherent allergens and toxic proteins. Rainbow papaya fruit were analyzed at three ripening stages and the data compared to that of a non-transgenic papaya which shares a similar pedigree. No differences were observed between GE and non-GE papaya for 36 nutrients at any of the tested fruit ripeness stages. However, vitamin A was higher and calcium levels were lower in the GE fruit. The GE fruit showed higher levels of protein and papain at the earliest stage of ripening (color break), but in ripened fruit these differences were insignificant. Benzyl isothiocyanate (BITC) levels were very low and similar for both Rainbow and the non-transgenic control fruit at all ripeness stages. Our data show that the contents of nutrients, BITC and papain of GE Rainbow papaya are within the range of those of non-GE papaya and that the Rainbow cultivar is substantially similar to the non-GE cultivar.     

木瓜黄精膏对衰老模型小鼠血清SOD活性及MDA含量的影响

目的:探讨木瓜黄精膏(PRO)对衰老模型小鼠血清SOD、MDA含量的影响。方法:把50只小鼠随机分为5组,除正常对照组外均每日注射D-半乳糖造成衰老模型,同时给予不同剂量的PRO。40日后检测小鼠血清中SOD活性、MDA含量。结果:衰老模型组与正常对照组比较,小鼠血清中SOD活性显著降低、MDA含量显著升高(P<0.01);PRO大、中剂量组与衰老模型组相比较,小鼠血清中SOD活性显著升高,MDA含量显著降低,有显著性差异(P<0.01)。结论:PRO具有较好的抗衰老作用。     

Functional Characterization of Cucumis metuliferus Proteinase Inhibitor Gene (CmSPI) in Potyviruses Resistance

Proteinase inhibitors are ubiquitous proteins that block the active center or interact allosterically with proteinases and are involved in plant physiological processes and defense responses to biotic and abiotic stresses. The CmSPI gene identified from Cucumis metuliferus encodes a serine type PI (8 kDa) that belongs to potato I type family. To evaluate the effect of silencing CmSPI gene on Papaya ringspot virus resistance, RNA interference (RNAi) with an inter-space hairpin RNA (ihpRNA) construct was introduced into a PRSV-resistant C. metuliferus line. CmSPI was down-regulated in CmSPI RNAi transgenic lines in which synchronously PRSV symptoms were evident at 21 day post inoculation. Alternatively, heterogeneous expression of CmSPI in Nicotiana benthamiana was also conducted and showed that CmSPI can provide resistance to Potato virus Y, another member of Potyvirus, in transgenic N. benthamiana lines. This study demonstrated that CmSPI plays an important role in resistant function against potyviruses in C. metuliferus and N. benthamiana.     

Bio—Normalizer（BN）对小鼠免疫功能调节作用的实验研究

目的;观察Bio-Normalizer(BN)对小鼠免疫功能的调节作用。方法：选节BALB／C纯系小白鼠分成3批共144只,随机分为对照、低剂量、中剂量和高剂量4个剂量组,其中低、中、高剂量组每天灌胃不同剂量的BN,对照组则每天灌胃同等数量的生理盐水,共观察3周。结果：低、中、高剂量组小鼠胸腺／体重比值和脾脏／体重比值均高于对照组,并有显著性差别（P＜0．05）;3个剂量组小鼠脾淋巴细胞增殖力均高于对照组,其中高剂量组小鼠脾淋巴细胞增殖力高于对照组,并有显著性差别（P＜0．05）;绵羊红细胞诱导小鼠DTH试验、低、中、高剂量小鼠24h,48h足跖增厚均高于对照组,并有显著性差别（P＜0．05）,中剂量和高剂量BN组NK细胞活性高于对照组,并有显著性差别（P＜0．05）。结论：BN具有免疫调节作用。     

木瓜粉对缺氧损伤神经细胞抗氧化能力的影响

目的：观察木瓜粉对缺氧损伤神经细胞抗氧化能力的影响。方法：将含木瓜粉培养液的神经细胞置于缺氧环境造成缺氧损伤,将细胞分为4组,I组为缺氧木瓜粉浓度为0mg/ml组,Ⅱ组为缺氧木瓜粉浓度为0．1mg/ml组,Ⅲ组为缺氧木瓜粉浓度为0．5mg/mlxeg ,Ⅳ组为非缺氧木瓜粉浓度为0mg/ml组,于细胞培养后的第4天收集细胞,进行有关指标的测定。结果：添加木瓜粉的Ⅱ,Ⅲ组其SOD活力和GSH－Px活性均高于缺氧0mg木瓜粉／ml组,缺氧0．5mg木瓜粉／ml组和非缺氧组MDA含量显著低于缺氧0mg木瓜粉／ml组,缺氧0．5mg木瓜粉／ml组TChE显著高于其它三组。结论：木瓜粉能够提高缺氧损伤神经细胞的抗氧化能力,促进神经细胞功能的恢复。     

Genome analysis of a severe and a mild isolate of <Emphasis Type="Italic">Papaya ringspot virus</Emphasis>-type W found in Brazil

Papaya ringspot virus-type W (PRSV-W) is one of the most economically threatening viruses of cucurbits in Brazil. Premunization is one of the most effective PRSV control measures currently applied in squash and zucchini crops. PRSV-W-1, a mild and premunizing strain of PRSV has been successfully used to protect cucurbits against both the severe PRSV-W-C strain and other Brazilian PRSVs. To aid in understanding the mechanism by which PRSV-W-1 premunization operates, the complete genome sequences of PRSV-W-1 and PRSV-W-C were determined. PRSV-W-1 had a genome size of 10,332 nucleotides, whereas indels within the coat protein encoding gene meant that the genome size of PRSV-W-C was six nucleotides shorter than that of the mild strain. The genomes of the two strains shared 94.63% nucleotide sequence identity, with the 5′ UTR and P1 being the most variable regions, and the coat protein and 3′ UTR being the most conserved. Rigorous recombination analysis revealed that neither PRSV-W-1 nor PRSV-W-C was obviously recombinant, there was significant evidence that many other fully sequenced PRSV genomes were recombinant. An erratum to this article can be found at