Evaluation of HbA1c on the Roche COBAS Integra 800 closed tube system

OBJECTIVES: Evaluate a new whole blood (WB) HbA1c immunoassay and system with closed tube sampling (CTS) capability. DESIGN AND METHODS: Compare the Tina-quant Haemoglobin A1c Gen.2 (A1C-2) application on the COBAS INTEGRA 800 (I800) and new I800 dedicated system with CTS capability to current Integra applications and a HbA1c method accurate with common haemoglobin (Hb) variants. RESULTS: CVs were < or =1.7%. Mean bias against National Glycohaemoglobin Standardization Program (NGSP) samples was 0.3 HbA1c %. Compared to the Hitachi Tina-quant(R) [a] HbA1c II (HbA1c II) assay (accurate with common Hb variants), mean bias was 0.04% and 0.21% HbA1c at 6% and 9%, respectively, with Hb AS variants; and -0.01% and 0.26% HbA1c at 6% and 9%, respectively, with Hb AC variants. CONCLUSIONS: The Integra A1C-2 application is precise, accurate against NGSP-assigned samples and the Hb variants tested; and, the I800 dedicated system with CTS capability offers increased throughput and reduced sample handling.     

�������ʷ��ڱ���ʽ����ֲ���е�Ӧ��

目的 总结在背驮式原位肝移植中运用刮吸解剖法的经验 。方法 实施背驮式肝移植中进行受体病肝切除术,从皮肤切口,分离肝周韧带,解剖一、二、三肝门,到保留肝 腔静脉而完全切除病肝的过程中的,全部使用刮吸解剖法来完成手术。１９９９年８月３１日施行第一例背驮式肝移植,１９９９年９月９日施行第二例背驮式肝移植,两例均患先天 生肝内胆管弥漫性囊性状扩张症伴屡发胆道感染和门脉高压伴食道静脉曲张破裂出血。结果 两     

Surgical treatment of hepatocellular carcinoma originating from caudate lobe—A report of 39 cases

The study objective was to study the therapeutic effect of surgical treatment for hepatocellular carcinoma (HCC) originating from the caudate lobe. From 1995 to 2003, caudate lobe resection was carried out for 97 cases; among them 39 were for HCC, who were divided into two groups. Group A consisted of 19 cases undergoing isolated caudatectomy, and group B consisted of 20 cases undergoing caudatectomy combined with other liver resections. The factors that might influence postoperative recovery were com-pared between the two groups. A special instrument, Peng’s Multifunctional Operative Dissector, was used for surgical dissection. All tumors were resected successfully. One patient died of postoperative re-nal failure. Hydrothorax occurred in three patients, ascites occurred in four patients, and bile leakage occurred in one patient. Thirty cases received long-term follow-up with survival rates at 1, 3, and 5 years of 53%, 50%, and 39%, respectively. Caudate lobectomy is an effective therapeutic method for HCC originating in the caudate lobe. Isolated caudatectomy should be performed as the first choice whenever possible. Anterior transhepatic approach is appropriate in some cases. Peng’s Multifunctional Operative Dissector is a very useful instrument for surgical dissection. Presented at the American Hepato-Pancreato-Biliary Association 2005 meeting, Hollywood, Florida, April 14–17, 2005. Supported by grants from Zhejiang Province Natural Science Fund, China (Fund No. 0306GJHZ).     

在基层医院应用多功能手术解剖器行胆囊切除术经验探讨

目的总结在基层医院用多功能手术解剖器及刮吸解剖法行胆囊切除术中的应用经验。方法把我院自2008年以来应用多功能手术解剖器及刮吸解剖法开展的胆囊切除术30例,按时间段进行分析,并与2007年同期28例使用普通电刀手术的数据进行对比。结果熟练使用多功能手术解剖器后能使手术难度降低,手术时间缩短,出血量减少。结论使用刮吸解剖法是一种简便、高效、实用的操作方法,适宜在基层外科推广使用。临床操作10例后即可掌握其基本操作要领,主要靠多训练才能体会和发扬其优点。     

螺旋水刀与多功能手术解剖器在肝门区肿瘤切除术中的联合应用

目的 探讨螺旋水刀与彭氏多功能手术解刮器(PMOD)在肝门区肿瘤切除术中的联合应用.方法 对35例联合应用螺旋水刀与PMOD在切除肝门区肿瘤的病人的手术资料进行回顾性分析,并与单纯应用螺旋水刀和PMOD进行比较.结果 PMOD组需肝门阻断21例;螺旋水刀组需阻断肝门5例;联合应用组需阻断肝门2例.术中视野清晰,肝内管道系统暴露充分,未发生管道系统误损,术后B超监测未发现有重要血管损伤的表现;PMOD组肿瘤切除时间较短,肝面烧灼止血面大,但出血量较大、术后肝功能恢复时间较长;而单纯应用螺旋水刀虽然肝功能恢复时间短,但切除肿瘤时间长;联合应用组肿瘤切除时间比螺旋水刀组短、出血量比PMOD组少、术后肝功能恢复时间比POMD组优.结论 螺旋水刀与彭氏多功能手术解剖器联合应用能安全、快捷切除肝门区肿瘤.     

提高肝门胆管癌切除率的探讨

该院连续收治肝门胆管癌１７例。由于手术器械（ＰＭＯＤ）和肝切开术（刮吸法）的改进，１４例得以切除，切除率达８２．３％。按照Ｂｉｓｍｎｕｔｈ分型，Ⅰ型２例、Ⅱ型５例、Ⅱａ５例、Ⅲｂ４例、Ⅳ型１例。切除的病例中，伴右半肝切除２例，伴左半肝切除２例，伴Ⅳ段切除２例，单纯肝正中裂切开８例。１７例中实施“骨骼化”切除３例。切除的１４例随访６～３６月，４例死亡，其余存活。文中简要介绍了用刮吸法切开肝正中裂的手术步骤和要点。肝外胆管切除后，肝面遗留的肝管开口应与空肠作Ｒｏｕｘ－Ｙ吻合。文中还介绍了“拼缝”、“运河”、“肝盆”等手术方法。     

彭氏电刀在鼻内镜下腺样体手术中止血的应用

目的探讨彭氏电刀在鼻内镜下经口鼻双径路腺样体手术中止血的临床应用价值。方法对行鼻内镜下经口鼻双径路腺样体手术的138例患者中78例出血者,采用彭氏电刀止血法。结果 78例患者术后均未行鼻咽部填塞,无再次出血,无其它并发症。结论彭氏电刀在鼻内镜下经口鼻双径路腺样体手术中的应用,止血快捷彻底、定位明确、术野清晰、操作简单,值得临床推广应用。     

刮吸手术解剖法在复杂胆道手术中的应用体会

目的总结在胆道手术中运用刮吸解剖法的手术治疗经验。方法 2007年1月至2011年12月,我科应用刮吸解剖法施行急诊胆囊切除术72例,肝十二指肠骨骼化清扫手术110例。结果 182例手术均获成功,无胆管损伤、胆漏、术后出血等并发症。肝十二指肠韧带骨骼化清扫手术中未出现胆管及血管的严重损失。结论应用刮吸解剖法施行胆道手术能缩短手术时间,操作更加精细,可减少术中出血,增加手术安全性。此法有助于肝门结构显露,增强了肝十二指肠韧带骨骼化程度,提高了根治性切除率。刮吸解剖法是一种实用、简便且安全的手术操作方法。。     

刮吸解剖法施行小切口胆囊切除加胆总管切开取石术

目的 探讨小切口胆囊切除加胆总管切开取石的可能性。方法 选择无肝内病变患15例，采用彭氏多功能手术解剖器，通过4－6厘米长的小切口胆囊切除加胆总管切开取石术。结果 15例成功完成小切口胆囊切除加胆总管切开取石术，手术创伤，出血均比大切口小，手术时间与大切口相当，术后病人恢复比大切口快，且美观，无医源性损伤发生。结论 小切口胆囊切除加胆总管切开取石术，在技术上是可行的，具有损伤小，痛苦轻，并发症少，安全性高，病人恢复快等优点。     

Subadventitial cystectomy in the management of biliary fistula with liver hydatid disease

Biliary fistulas are the most common morbidity (8.2–26%) following hydatid liver surgery. The aim of this study was to evaluate the results of subadventitial cystectomy in the treatment of liver hydatid cyst associated with a biliocystic fistula. The medical records of 153 patients who underwent subadventitial cystectomy for a liver hydatid cyst between January 2006 and December 2010 were retrospectively reviewed. Cysts were located in the right lobe anterior segment 37 (24.2%) patients, right lobe posterior segment 59 (38.6%) patients, the left lobe in 26 (17.0%) patients, and both lobes in 6 (3.9%) patients. The surgical procedures performed were closed (non-incised) subadventitial total cystectomy in 74 patients (48.4%), open (incised) subadventitial total cystectomy in 30 patients (19.6%), and subadventitial subtotal cystectomy in 49 patients (32.0%). Biliocystic communication was found in 52 patients (34.0%), and 21 patients (13.7%) were treated with T-tube drainage. Two patients had performed biliodigestive anastomosis. Biliary fistula was detected in 9 patients after subtotal subadventitial cystectomy. Biliary fistulas closed spontaneously within 10 days and 61 days respectively and the amount of drainage varying between 50 and 400 ml after the procedure. Postoperative complication and recurrence rates were 19.0% and 0.7%, respectively. The mortality rate was 0%. Subadventitial cystectomy should be the surgical treatment of choice for this disease because of its feasibility and low rates of recurrence, complications of the residual cavity, and incidence of associated biliary fistula.