NF-κB对尿白蛋白诱导肾小管上皮细胞骨桥蛋白转录的影响

目的研究NF-κB对尿白蛋白诱导肾小管上皮细胞骨桥蛋白转录的影响。方法去脂的小牛血清白蛋白(BSA)20mg/ml分别加入到体外培养的肾小管上皮细胞和加入NF-κB抑制剂(PDTC)共同培养半小时的肾小管上皮细胞内,EMSA、RT-PCR检测不同时限NF-κB活性、骨桥蛋白mRNA表达情况。结果去脂的小牛血清白蛋白可刺激肾小管上皮细胞内NF-κB活性增加,骨桥蛋白mRNA表达上调,而提前加入NF-κB抑制剂(PDTC)共同培养半小时的肾小管上皮细胞内NF-κB活性、骨桥蛋白mRNA表达明显下降。结论尿白蛋白诱导小管上皮细胞损伤的机制与诱导肾小管上皮细胞内NF-κB活性、骨桥蛋白mRNA增加有关,而且骨桥蛋白转录由NF-κB调控。     

Saccades and multisaccadic gaze shifts are gated by different pontine omnipause neurons in head-fixed cats

 Pontine omnipause neurons (OPNs) have so far been considered as forming a homogeneous group of neurons whose tonic firing stops during the duration of saccades, when the head is immobilized. In cats, they pause for the total duration of gaze shifts, when the head is free to move. In the present study, carried out on alert cats with fixed heads, we present observations made during self-initiated saccades and during tracking of a moving target which show that the OPN population is not homogeneous. Of the 76 OPNs we identified, 39 were found to have characteristics similar to those of previously described neurons, ”saccade” (S-) OPNs: (1) the durations of their pauses were significantly correlated with the durations of saccades; (2) the discharge ceased shortly before saccade onset and resumed before saccade end; (3) visual responses to target motion were excitatory; and (4) during tracking, S-OPNs interrupted the discharge for the duration of saccades and resumed firing during perisaccadic ”drifts”. However, the characteristics of 37 neurons (”complex” (C-) OPNs) were different: (1) the pause duration was not correlated with the duration of self-initiated saccades; (2) time lead of pause onsets relative to saccades was, on average, longer than in the group of S-OPNs, and firing resumed after the saccade end; (3) visual target motion suppressed tonic discharges; and (4) during tracking, firing was interrupted for the total duration of gaze shifts, including not only saccades but also perisaccadic ”drifts”. We conclude that cat OPNs can be subdivided into two main groups. The first comprises neurons whose firing patterns are compatible with gating individual saccades (”saccade” OPNs). The second group consists of ”complex” OPNs whose firing characteristics are appropriate to gate total gaze displacements rather than individual saccades. The function of these neurons may be to disinhibit pontobulbar circuits participating in the generation of saccade sequences and associated perisaccadic drifts. Received: 20 January 1998 / Accepted: 22 October 1998     

泽泻有效部位对肾草酸钙结石模型大鼠肾组织骨桥蛋白表达的影响

目的研究泽泻有效部位对大鼠肾草酸钙结石形成和肾组织骨桥蛋白(OPN)表达的影响,探讨泽泻抑制尿结石形成的机制。方法采用现代植化和生物活性导向分离的方法分离、提取泽泻的有效部位。将30只大鼠随机分成3组对照组、模型组、泽泻组。以乙二醇和1α-羟基维生素D3ig制备大鼠肾草酸钙结石模型。检测各组大鼠血及尿生化、肾Ca2 水平、24h尿草酸分泌量和肾组织病理学改变,并采用免疫印迹(Western blotting)和半定量逆转录聚合酶链反应(RT-PCR)分别观察各组大鼠肾组织OPN蛋白及其mRNA的表达水平。结果ig泽泻有效部位(主要以四环三萜类化合物为主)大鼠的血清肌酐(SCr)、尿素氮(BUN)、肾Ca2 水平、24h尿Ca2 分泌量、肾组织草酸钙晶体的分布、OPN蛋白及其mRNA的表达水平均显著低于模型组(P<0.01)。结论泽泻有效部位能抑制大鼠肾组织OPN的表达,减少肾组织草酸钙结晶的沉积,从而能有效抑制大鼠肾草酸钙结石的形成。     

血清骨桥蛋白和CA125联合检测鉴别良恶性腹水

目的：探讨联合检测血清骨桥蛋白（OPN）和CA125在良恶性腹水中的诊断价值。方法：取39例恶性腹水患者、48例结核性腹水患者及40例健康妇女血清样品,分别用双抗体夹心酶联免疫吸附法（ELISA）及微粒子化学发光法测定其OPN和CA125水平,观察其在诊断良、恶性腹水时的敏感性和特异性。结果：恶性腹水患者血清OPN水平明显高于结核性腹水患者及健康妇女组。OPN检测恶性腹水的敏感度、特异度分别为84.6%、95.0%;CA125检测敏感度、特异度为76.9%、87.5%;联合检测敏感度97.4%、特异度为95.0%。联合检测OPN、CA125较单独检测OPN及CA125其敏感度高（P〈0.05）;特异度无明显差异。结论：OPN与CA125联合测定是鉴别卵巢癌和结核性腹水的有效方法,联合检测CA125可提高其临床应用价值。     

消癌平胶囊联合顺铂和替加氟治疗晚期食管癌的临床研究

目的探讨消癌平胶囊联合顺铂和替加氟注射液治疗食管癌的临床疗效。方法选取2016年4月—2018年4月在重庆市开州区人民医院治疗的食管癌患者86例,根据用药方案的差别分为对照组(43例)和治疗组(43例)。对照组静脉滴注顺铂注射液,80 mg/m~2,1次/d,连续3周,休息1周,每4周为1个周期,同时静脉滴注替加氟注射液,15～20 mg/kg溶于生理盐水500 mL中,1次/d。治疗组在对照组基础上口服消癌平胶囊,4粒/次,3次/d。两组患者均治疗3个周期。观察两组患者临床疗效,同时比较治疗前后两组患者癌胚抗原(CEA)、鳞状上皮细胞癌抗原(SCC-Ag)、细胞角蛋白19的可溶性片段(CYFRA21-1)、糖抗原199(CA199)、血管内皮生长因子(VEGF)、基质金属蛋白酶-9(MMP-9)、骨桥蛋白(OPN)、可溶性白细胞介素-2受体(s IL-2R)及QLQ-C30和KPS评分。结果治疗后,对照组客观缓解率和临床获益率分别为41.86%、72.09%,均分别显著低于治疗组的69.77%、86.05%,两组比较差异均具有统计学意义(P0.05)。治疗后,两组患者血清CEA、SCC-Ag、CYFRA21-1、CA199、VEGF、MMP-9、OPN、sIL-2R水平均显著降低(P0.05),且治疗组患者这些因子水平明显低于对照组(P0.05)。治疗后,两组QLQ-C30量表和KPS评分均明显升高(P0.05),且治疗组患者QLQ-C30和KPS评分明显高于对照组(P0.05)。结论消癌平胶囊联合顺铂与替加氟治疗食管癌可有效改善患者症状,降低机体肿瘤标志物水平和侵袭细胞因子水平,有利于提高患者生活质量。     

OPN及HE4基因在上皮性卵巢癌中的表达及临床意义

目的探讨卵巢癌中OPN蛋白及HE4蛋白表达与临床病理因素间的关系及其预后的影响。方法应用免疫组织化学SP法检测47例上皮性卵巢癌、15例卵巢上皮交界性肿瘤及10例卵巢上皮性良性肿瘤组织中OPN及HE4蛋白的表达情况,分析蛋白表达与临床病理因素间的关系及其对预后的影响。结果OPN及HE4蛋白的表达呈正相关,与临床分期、腹水、淋巴结转移密切相关,P<0.05。结论OPN及HE4基因蛋白高表达可能与上皮性卵巢癌浸润、侵袭、淋巴结转移有关。     

OPN基因多态性与潮汕人群女性SLE发病风险的相关性

目的 探讨骨桥蛋白(OPN)基因三个单核苷酸多态性(SNP)位点rs1126772、rs11728697和rs4754与潮汕人群女性系统性红斑狼疮(SLE)发病风险的相关性.方法 选取2018年1月至2019年12月在汕头大学医学院附属潮州市人民医院以及汕头大学医学院第一附属医院就诊的59例SLE女性患者作为研究组,选...     

骨调素和单核细胞趋化蛋白-1在糖尿病大鼠肾组织中的表达

目的观察糖尿病大鼠肾小管骨调素(OPN)、单核细胞趋化蛋白-1(MCP-1)表达的动态变化,探讨它们在糖尿病肾病(DN)发生发展中的作用。方法把链脲佐菌素诱导的糖尿病大鼠随机分为五组,每组设正常对照组。免疫组化法检测肾小管OPN、MCP-1和纤维连接蛋白(FN)的表达;PAS染色,光镜观察肾组织形态;生化方法测血糖、血肌酐及尿蛋白。结果OPN、MCP-1主要表达在肾小管上皮细胞,随着糖尿病病程发展,肾小管OPN、MCP-1和FN表达逐渐增加。结论OPN和MCP-1在糖尿病大鼠肾组织表达明显增加,可能参与了DN的发病机制。     

创伤后感染致多器官功能障碍综合征患者血清β-hBD-2、OPN、pro-ADM和NPt水平的变化

目的：探讨多器官功能障碍综合征（multipleorgandysfunctionsyndrome,MODS）患者的炎症感染病理过程中炎性介质水平的变化。方法：血清人类B防御素-2（humanB．defensin-2,β—hBD-2）、骨桥蛋白（osteopontin,OPN）和新蝶呤（neoptefin,NPt）采用酶联免疫分析法（ELISA）测定,肾上腺髓质素前体（pro-adrenomeduUin,pro—ADM）检测采用化学免疫发光法。结果：四项血清标志物测定结果分别显示,对照组和确诊时组B-hBD-2、OPN、pro-ADM、NPt四种血清炎症介质标志水平均显著高于正常人组（P〈0．05,P〈0．01）,出院前组β-hBD-2、OPN水平显著高于正常人组,另两项指标均与正常人组比较无显著性差异（P均〉0．05）;同时确诊时组与对照组比较β—hBD-2、OPN、pro-ADM三项指标水平也显著升高（P〈0．05,P〈0．01）,NPt水平患者组呈略高,但无统计学意义（P〉0．05）;出院前组β-hBD-2、OPN、pro-ADM、NPt四种血清炎症介质标志水平均显著低于确诊时组（P〈0．05,P〈0．01）。结论：MODS患者的炎症感染病理过程中炎性介质水平显著升高,其测定对于临床抗感染治疗提供重要价值。     

Correlation between calcification and bone sialoprotein and osteopontin in papillary thyroid carcinoma

The correlation between calcification and papillary thyroid carcinoma has received increasing attention. We investigated the ability of bone sialoprotein (BSP) and osteopontin (OPN) protein levels to diagnose papillary thyroid carcinoma (PTC), and explored the correlation between BSP and OPN protein levels and calcification in PTC. Archival PTC specimens from patients with PTC with calcification and lateral cervical lymph node metastasis (LNM) were included in this retrospective immunohistochemical study. The protein levels of BSP and OPN were analysed immunohistochemically using routinely prepared tissue sections. PTC specimens from 66 patients with PTC were reviewed retrospectively (25 patients with histological calcification seen in paraffin sections, 41 patients without calcification; 35 patients with lateral cervical LNM, 31 patients without LNM). The percentage of samples that had cells that demonstrated positive protein staining differed significantly between PTC specimens, benign thyroid nodules, and adjacent normal follicular epithelium (BSP: 87.88%, 55.00%, and 42.50%, respectively; OPN: 83.33%, 70.00% and 50.00%, respectively). There was a significant difference in the immunohistochemical score (IHS) for BSP and OPN protein staining between PTC specimens with and without calcification (P < 0.05). The level of BSP protein staining was found to be significantly correlated with the level of OPN protein staining in PTC specimens. We conclude that the strong correlation between BSP and OPN and PTC suggests a role for BSP and OPN in calcification and tumor progression of PTC. BSP and OPN might be useful tumour markers for the diagnosis of PTC with limited value, because both of them had low specificity.