Cardioprotective effects of sinomenine in myocardial ischemia/reperfusion injury in a rat model

BackgroundIschemia reperfusion (I/R) play an imperative role in the expansion of cardiovascular disease. Sinomenine (SM) has been exhibited to possess antioxidant, anticancer, anti-inflammatory, antiviral and anticarcinogenic properties. The aim of the study was scrutinized the cardioprotective effect of SM against I/R injury in rat.MethodsRat were randomly divided into normal control (NC), I/R control and I/R + SM (5, 10 and 20 mg/kg), respectively. Ventricular arrhythmias, body weight and heart weight were estimated. Antioxidant, inflammatory cytokines, inflammatory mediators and plasmin system indicator were accessed.ResultsPre-treated SM group rats exhibited the reduction in the duration and incidence of ventricular fibrillation, ventricular ectopic beat (VEB) and ventricular tachycardia along with suppression of arrhythmia score during the ischemia (30 and 120 min). SM treated rats significantly (P < 0.001) altered the level of antioxidant parameters. SM treatment significantly (P < 0.001) repressed the level of creatine kinase MB (CK-MB), creatine kinase (CK) and troponin I (Tnl). SM treated rats significantly (P < 0.001) repressed the tissue factor (TF), thromboxane B2 (TXB2), plasminogen activator inhibitor 1 (PAI-1) and plasma fibrinogen (Fbg) and inflammatory cytokines and inflammatory mediators.ConclusionOur result clearly indicated that SM plays anti-arrhythmia effect in I/R injury in the rats via alteration of oxidative stress and inflammatory reaction.     

白细胞介素-8在哺乳动物生殖过程中的作用

白细胞介素-8(IL-8)作为中性粒细胞趋化因子和促血管生成因子,受到人们的重视。研究表明,IL-8促进卵细胞的发育、成熟,诱发排卵;在输卵管和子宫内膜的表达随月经周期而变化;参与胚泡着床和胚胎发育。本文综述IL-8在哺乳动物生殖过程中的重要作用。     

异位子宫内膜和在位子宫内膜分泌泌乳素的研究

作者将29例子宫内膜异位症（简称异位症）患者的异位和在位内膜进行离体组织孵育，应用放射免疫方法检测孵育液中泌乳素（PRL）含量；同时应用ABC免疫酶标记法确定异位和在位内膜分泌PRL的部位，结果异位和在位内膜离体组织经0～24h孵育，孵育液中PRL含量均显著增加（P＜0．05）；继续孵育至48h，PRL含量不再增加。但异位内膜分泌PRL的活性低于自身在位内膜。免疫组化PRL检测，异位和在位内膜分别有4和6例呈PRL阳性反应。组织学对照现察，内膜成熟上皮细胞数及前蜕膜细胞数越多者，其相应孵育液中PRL含量越高，免疫组化PRL阳性反应也越强。提示异位内膜PRL分泌特点与其自身在位内膜不完全相同，可能与病理环境有关。     

Successful pregnancy after recurrent hysteroscopic polypectomy

This case demonstrates that hysteroscopic polypectomy can enhance fertility. A 35-year-old woman was being evaluated for assisted conception. She was referred from an in vitro fertilisation clinic for polypectomy, which she had undergone on two occasions. She conceived spontaneously, had an uneventful antenatal period, and delivered a healthy baby at 39 weeks' gestation.     

精神病人与正常人戒烟前后焦虑抑郁症状的对照研究

目的　了解精神病人和正常人戒烟前后精神状况的变化及差异性 ,探讨戒烟措施。方法　对 71例精神病人和 5 0例正常人戒烟前及戒烟后 1周分别应用汉密顿焦虑量表 (HAMA)、汉密顿抑郁量表 (HAMD)、焦虑自评量表 (SAS)、抑郁自评量表 (SDS)、简明精神病量表 (BPRS )进行测评。结果　精神病人强制性戒烟前HAMA、HAMD、SAS、SDS、BPRS分值分别为 (8.2 1± 6 .4 1)、(7.6 4± 5 .71)、(36 .81± 7.14 )、(33.71± 7.1)、(2 9.4 5± 8.4 7) ;戒烟后HAMA、HAMD、SAS、SDS、BPRS分值分别为 (16 .4 5± 6 .34)、(19.73± 8.71)、(5 8.1± 12 .12 )、(5 6 .31± 11.4 )、(37.32± 7.95 ) ;戒烟前后 5种量表分值变化与正常人戒烟前后分值变化比较有极显著性差异(P <0 .0 1)。结论　对精神病人强制性戒烟可引起明显的情绪反应 ,应适宜控制病人吸烟 ,建立一个合理的管理制度     

经阴道超声及宫腔造影对鉴别子宫内膜息肉与其他病变的意义

目的分析子宫内膜病变的声像图和血流特征,并评价经阴道彩色多普勒超声宫腔造影对子宫内膜息肉的诊断价值。方法采用经阴道彩色多普勒加宫腔造影,分别对19例子宫内膜息肉和32例子宫内膜其他病变之声像图、彩色血流及频谱表现进行对比,全部病例经宫腔镜及手术后的病理证实。结果经阴道彩色多普勒超声检诊子宫内膜息肉,清晰显示了内膜与黏膜下肌层之界限和息肉的病变边界,还显示了息肉蒂基底的彩色血流或黏膜下肌瘤周边彩色血流环,适时加做宫腔造影则更有益于对细小病变的诊断。结论经阴道彩色多普勒超声加做宫腔造影可大大提高对子宫内膜息肉与子宫内膜其他病的鉴别诊断水平。     

人子宫内膜植入兔眼前房模型的研究

本实验成功地将人子宫内膜植入到１５只去卵巢新西兰雌兔的眼前房，并给每只兔外源补充雌二醇（Ｅ２）１００μｇ／ｄ和孕酮（Ｐ）８ｍｇ／ｄ。在内膜植入期间每只兔皮下注射总量为９ｍｇ的霉酚酸（ｍｙ－ｃｏｐｈｅｎｏｌｉｃａｃｉｄ）以防免疫排斥反应。在眼前房的人子宫内膜存活了２８．４４±８．６５天，兔血清Ｅ２和Ｐ的平均值分别为１６１．７１±１５．１３和３８．８８±７．４５ｐｍｏｌ／Ｌ。前房液中Ｅ２和Ｐ浓度在植入人子宫内膜前后分别为２４．８０±１６．５３和１７０９．２５±４７５．１０ｐｍｏｌ／Ｌ，２．００±０．５３和１２．２２±３．８１ｎｍｏｌ／Ｌ。树脂切片验证人子宫内膜植入兔眼前房第５天就能与虹膜附贴并有血管发生。来自巩膜的血管分布在子宫内膜周围。扫描电镜观察人子宫内膜分化成分泌细胞和纤毛细胞。植入人子宫内膜后，前房液的蛋白浓度高于植入前。可以证明该模型可用来研究人子宫内膜对药物的反应和通过对前房液的测量研究子宫内膜的分泌功能。     

Renal vascular resistance in normal children – a color Doppler study

To investigate the relationship between age and renal vascular resistance and to establish nomative data of the systolic/diastolic ratio (S/D ratio), pulsatility index (PI), and resistant index (RI) of the renal artery in the normal pediatric population, we studied 252 normal children aged newborn to 13 years (a total of 449 kidneys) with a color Doppler unit. After the normality of the kidney was established, color flow mapping was performed to localize the segmental portion of the renal artery. Flow velocity waveforms were then obtained by pulsed Doppler, and S/D ratio, PI, and RI were calculated. Multiple regression analysis confirmed the age dependence of the S/D ratio, PI, and RI of the renal artery in normal children. Renal vascular resistance continuously declines after birth and stabilizes at the age of 102 – 130 months. Normative data for S/D ratio, PI, and RI of the renal artery in normal children were established for each age group. Since renal vascular resistance decreases with age and stabilizes at 8 – 10 years, we suggest using different normal ranges for each age group when studying renal vascular resistance in pediatric patients. Received October 5, 1995; received in revised form and accepted April 24, 1996     

实验小鼠肠道正常菌群

在动物肠道中栖息的细菌，构成了肠道正常菌群。实验动物的质量与肠道菌群的状态有着十分密切的联系，肠道菌群平衡状态在维持动物机体的生理状态、正常功能，以及抵抗外袭菌方面起着重要的作用。小鼠肠道中，每克肠内容物含有上亿个菌体，可分为10～15个属，上百个种。肠道正常菌群的主要菌属有双歧杆菌、拟杆菌(类杆菌)、肠球菌、乳杆菌、梭杆菌、真杆菌。不同品系的小鼠之间，各种菌的数量有所不同。本文简要介绍了近年来对小鼠肠道正常菌群的研究进展。     

正常成人脑海马区多潜能神经前体细胞的体外分离鉴定

目的 从正常成人脑海马分离鉴定多潜能神经前体细胞并探讨体外培养条件.方法 材料取自8例非神经系统疾病的死亡者.分离正常成人脑海马细胞,在培养基中添加生长因子体外培养.通过细胞培养成球和BrdU染色鉴定培养细胞的增殖能力.利用免疫细胞化学三标染色来鉴定分化神经细胞的表型.结果 从正常人海马分离的细胞培养2周时,开始增殖成簇,致一个月时,形成细胞球,这些细胞能与BrdU结合,并能在体外分化成神经系统不同谱系的细胞.结论 与在啮齿类动物和猴的研究中结果一致,从正常成人脑海马区分离的细胞是多潜能神经前体细胞.