氧氟沙星阴道泡腾片抗菌活性实验动物的研究

采用氧氟沙星（ＯＦＬＸ）阴道泡腾片和诺氟沙星（ＮＦＬＸ）阴道栓对临床常见的细菌性阴道炎进行了动物体内外抗菌活性测定。结果显示：体外测定组：ＯＦＬＸ对致病菌的ＭＩＣ在０１～３１２μｇ／ｍｌ而ＮＦＬＸＭＩＣ与其相近。体内测定组：ＯＦＬＸ的治愈率２ｍｇ／ｋｇ组为５０％，４ｍｇ／ｋｇ和８ｍｇ／ｋｇ组为１００％。而ＮＦＬＸ栓２ｍｇ／ｋｇ组为４０％，４ｍｇ／ｋｇ组为８０％，８ｍｇ／ｋｇ组为１００％，空白组治愈率为０。说明ＯＦＬＸ阴道泡腾片对此类阴道炎具良好的疗效，低、中剂量组略优于ＮＦＬＸ栓。但两药无统计学差异     

Effect of chronic fluoxetine treatment on male and female rat erythrocyte and prefrontal cortex fatty acid composition

Omega-3 (n-3) polyunsaturated fatty acids (PUFA) and fluoxetine (FLX) have additive effects in the treatment of major depressive disorder, and FLX up-regulates genes that regulate fatty acid biosynthesis in vitro. Although these data suggest that FLX may augment n-3 fatty acid biosynthesis, the in vivo effects of FLX treatment on PUFA biosynthesis and peripheral and central membrane compositions are not known. In the present study, male and female rats were treated with FLX (10 mg/kg/day) through their drinking water for 30 days (P60–P90). Plasma FLX and norfluoxetine (NFLX) concentrations were determined by liquid chromatography tandem mass spectrometry, and erythrocyte and prefrontal cortex (PFC) fatty acid composition determined by gas chromatography. To confirm central effects of FLX, serotonin turnover in the PFC was determined by high performance liquid chromatography. Chronic FLX treatment resulted in clinically-relevant plasma FLX concentrations in male and female rats, and significantly decreased serotonin turnover in the PFC. After correcting for multiple comparisons, chronic FLX treatment did not significantly alter erythrocyte fatty acid composition in male or female rats. Chronic FLX treatment significantly and selectively increased docosapentaenoic acid (22:5n-6) in the PFC of female rats, but not in male rats. These preclinical findings do not support the hypothesis that chronic FLX treatment increases n-3 fatty acid biosynthesis or membrane composition.     

HPLC法优选NFLX和PFLX混合物的分离条件

目的：用高效液相色谱法优选NFLX和PFLX的分离条件。方法：ODS柱，以甲醇-乙酸钠(0．25M)为流动相，于275nm波长处检测，流速为1．0ml／min。结果：NFLX和PFLX混合物的分离条件为，甲醇：乙酸钠缓冲液=70：30，缓冲液PH值为5．7。结论：此方法简便，快速，重现性好。