The effect of the lipophilic cation lucigenin on mitochondria depends on the site of its reduction

The role of NAD(P)H-dependent oxidoreductases of the outer mitochondrial membrane (OMM) in the activation of lipophilic cationic dyes is poorly understood. In the present study we compared the rates of production of reactive oxygen species (ROS) and mitochondriotoxic effects of the redox-cycling lipophilic cationic dye lucigenin upon its activation by the respiratory chain and NAD(P)H-dependent oxidoreductases of the OMM. We found that, only in the presence of external NADH and NADPH, which are unable to penetrate the inner membrane, lucigenin stimulated a massive superoxide production and a fast permeabilization of mitochondrial membranes. The permeabilization was biphasic. The first, cyclosporin A-insensitive and Ca(2+)-independent phase was characterized by increased permeability of the inner mitochondrial membrane to solutes with molecular masses of 相似文献   

Reliability of checklist-guided diagnoses for DSM-IIIR affective and anxiety disorders

The test-retest reliability of DSM-IIIR diagnoses for affective and anxiety disorders was determined under clinical routine conditions in a psychiatric outpatient department. The sample consisted of 60 patients, and the Munich Diagnostic Checklists (MDCL) were administered for diagnostic evaluation and classification. Each subject was independently examined by two of four participating diagnosticians (two psychiatrists, two psychologists). Acceptably high levels of agreement were indicated by several statistics (including k) for most disorders. Reliability was analyzed for diagnoses, subclassifications, and symptoms. Reduced agreement was found only for dysthymia, agoraphobia, and social phobia. Major causes were information variance and weaknesses of operationalization. Overall results were satisfactory when compared with other reliability studies.     

Cardiovascular Disease Among Women From Vulnerable Populations: A Review

On a global scale, cardiovascular disease (CVD) is the leading cause of mortality. It is also the number 1 cause of death among women, resulting in 8.6 million deaths annually and constituting one third of all deaths in women worldwide. The burden of CVD and related risk factors has taken priority in the policy development for noncommunicable diseases. However, vulnerable populations, defined here as women who are socially or economically disadvantaged (eg, low income), nonwhite (specifically South Asian and indigenous women), and those who are elderly have often been overlooked in these discussions. These additional vulnerabilities, which may exist independently or in combination, place such women at higher risk for CVD. Specifically, these vulnerabilities include low socioeconomic status, a low sense of control, high stress, South Asian or indigenous ancestry, and increased age. Thus it is vital that we initiate a multipronged approach to CVD prevention that includes rigorous monitoring of CVD risk factors in high-risk populations and the implementation of timely, accurate, and contextually tailored prevention programs, services, and treatments. Well-trained nonphysician health care workers can support the accurate monitoring and management of CVD and CVD risk factors so that groups of women who may otherwise be overlooked can receive adequate attention.     

DRGs在肾脏及泌尿系统疾病住院医疗绩效评价中的应用

目的 应用诊断相关组对医院学科发展不足领域肾脏及泌尿系统疾病进行住院医疗绩效评价,为医院加强精细化管理和学科建设提供数据支持.方法 以国家版DRGs(CN-DRGs)分组方式,运用CMI、DRGs组数、费用消耗指数、时间消耗指数、低风险组死亡率等指标对2015年-2019年医院出院病例进行分析.结果 2015年-201...