Cellular dynamics of corneal wound re-epithelialization in the rat. I. Fate of ocular surface epithelial cells synthesizing DNA prior to wounding

The fate of ocular surface epithelial cells in response to injury of the cornea was examined. Corneal epithelial cells were labeled during DNA synthesis with [3H]thymidine 1 h prior to wounding. A 3-mm diameter epithelial defect was made in the center of the rat cornea, with the basement membrane remaining intact. Within 12 h of abrasion, labeled cells were detected in the regenerating surface. At 18 h, there was a 2.7- and 17-fold increase of labeled basal and suprabasal cells, respectively, in the epithelium adjacent to the wound, and at 24 and 30 h there was an excessive number of cell layers (up to 7) at the margin of the abrasion. Re-epithelialization progressed as a gradient of cell layers that became diminished towards the center of the wound. Completion of layers 1, 2, 3, and 4 were recorded at 24, 30, 36, and 72 h, respectively. No changes in the labeling index of the limbus or conjunctiva were noted. These results suggest that processes of centripetal and vertical migration, as well as events related to cell division, in the uninjured corneal surface are not impeded by wounding of the corneal epithelium. However, wound healing appears to require cells with a basal phenotype, presumably because of this cell type's migratory capability.     

自体角膜缘移植术治疗复发性翼状胬肉疗效观察

目的　观察自体角膜缘移植术治疗复发性翼状胬肉的疗效。方法　对 10例 (10眼 )复发性翼状胬肉患者施行患眼健侧带结膜的自体角膜缘移植术。结果　随访 6～ 12个月 ,10例患者角膜移植片均透明光滑 ,愈合良好 ,无一例复发。结论　自体角膜缘移植术治疗复发性翼状胬肉有效、安全、简便。     

胬肉头部转位加角膜缘干细胞移植治疗翼状胬肉113例疗效观察

【目的】观察翼状胬肉头部转位＋角膜缘干细胞移植治疗翼状胬肉的疗效。【方法】对113例（140只眼）翼状胬肉患者，采用翼状胬肉头部转位＋角膜缘干细胞移植手术，观察术后胬肉复发及并发症情况，随诊3～12个月。【结果】113例（140只眼）除17眼失访者外，有4只眼出现复发，复发率3．25％，无一眼出现感染、植片坏死等并发症，少部分患者有长期眼部异物感等不适。【结论】翼状胬肉头部转位＋角膜缘干细胞移植是治疗翼状胬肉的一种有效方法，适于临床广泛应用。     

自体带角膜缘干细胞结膜瓣移植治疗翼状胬肉临床分析

目的:探讨自体带角膜缘干细胞结膜瓣移植治疗翼状胬肉的临床疗效.方法:翼状胬肉175例(182眼),其中进展期161眼,复发性9眼,在显微镜下行胬肉组织切除联合自体带角膜缘干细胞的结膜瓣移植重建角膜缘,术后对角结膜创面修复生长情况跟踪观察.结果:经过术后平均2年的随访观察角结膜创面愈合良好,4例复发,复发率2.20%.结论:常规胬肉切除联合自体带角膜缘干细胞结膜瓣移植重建角膜缘术对降低胬肉复发率的效果是肯定的.     

角膜缘干细胞移植联合丝裂霉素C治疗复发性翼状胬肉

目的 观察自体角膜缘干细胞移植联合丝裂霉素C治疗复发件翼状胬肉的疗效.方法 将58例(62眼)复发性翼状胬肉患者随机分为2组,治疗组(32例,35跟)采用角膜缘于细胞移植联合应用丝裂霉素C;对照组(26例,27眼)单纯采用角膜缘干细胞移植术治疗.术后随访6～30个月并比较疗效.结果 治疗组1眼冉复发,再复发率2.86%;对照组5眼再复发,再复发率18.52%,两组差异具有统计学意义(P<0.05).治疗组角膜创面上皮平均愈合时间为(8.34 ±2.38)d,对照组为(7.21±2.55)d,差异无统计学意义(P>0.05).两组均未发现严重并发症.结论 角膜缘干细胞移植联合应用丝裂霉素C治疗复发性翼状胬肉再复发率低,并发症少,为复发性翼状胬肉安全和有效的治疗方法 .     

白内障手术角巩膜缘切口和透明角膜缘切口的疗效比较

目的比较角巩膜缘切口和透明角膜缘切口白内障超声乳化吸除、人工晶体植入术后的疗效。方法选取本院2011年1月至2014年1月收治的120例白内障患者,所有患者均行超声乳化、人工晶体植入术。将患者随机分为观察组与对照组,每组各60例。观察组采用角巩膜缘切口,对照组采用透明角膜缘切口,观察两组患者治疗后d1、术后1周、1个月、3个月的视力、散光程度以及并发症情况。结果观察组术后各时间段裸眼视力显著优于对照组,角膜散光程度也显著小于对照组,差异均有统计学意义（均 P ＜0．05）;两组患者在并发症发生情况上无显著性差异（ P ＞0．05）。结论对白内障患者实施超声乳化吸除、人工晶体植入术,使用改良角巩膜缘切口超声乳化吸除的效果显著优于透明角膜缘切口,患者术后视力恢复情况好,角膜散光程度较小,值得临床推广应用。     

The limbus spiralis and its relationship to the developing tectorial membrane in the cochlear duct of the guinea pig fetus

Summary The development of the interdental cells of the limbus spiralis and of the inner spiral sulcus cells as well as the formation of the mesenchymal teeth of Huschke are described during fetal life up to the day of birth in the guinea pig. Additionally, the changes of the developing tectorial membrane are studied. The ultrastructural observations allow the conclusion that during fetal development at least a considerable part of the material of the tectorial membrane is secreted by the interdental cells of the limbus spiralis.Supported by Deutsche Forschungsgemeinschaft     

Rezidivierende Erosio corneae nach mechanischen Traumata Ergebnisse der transepithelialen phototherapeutischen Keratektomie

Background. Recurrent corneal erosion is a common clinical disorder characterised by repeated spontaneous breakdown of the corneal epithelium. The pathomechanism is due to abnormalities of the adhesion complex between the corneal epithelium and the stroma. In addition to local medical treatment, bandage lenses, debridement and anterior stromal puncture, patients can also be treated by excimer laser phototherapeutic keratectomy. Methods. We present a series of 45 eyes where a transepithelial therapeutic excimer photoablation (t-PTK) was carried out after recurrent erosions caused by mechanical trauma. We used the flying spot excimer laser Technolas 217-C (Bausch&; Lomb Surgical) and the scanning spot laser MEL70 (Aesculap Meditec). Treatment was performed in the relapse-free period. The zone of ablation measured 6–9 mm and the ablation depth10–30 μm. The mean follow-up period was 7 months (range 3–22). Results. After the first t-PTK treatment, 41 eyes remained without a relapse, but 8 patients had persistent symptoms, 4of which had a new epithelial breakdown and in 2 patients a second t-PTK had to be carried out. Conclusions. T-PTK appears to be a safe and effective procedure for treatment of recurrent corneal erosion. However, relative contraindications, such as a previous chemical burn, must be taken into account.     

Annulus fibrosus calcification in the cervical spine: radiologic — pathologic correlation

Intervertebral disc calcification can be secondary to a variety of pathologic processes including some of the arthritidies, metabolic diseases, and trauma. The annulus fibrosus is the most commonly calcified component and may mimic vertebral body fracture, limbus vertebra, or a persistent ring apophysis. We describe two young patients who developed calcification in the anterior annulus fibrosus and present radiologic-pathologic correlation in this condition in another case. This calcification may actually be secondary to subclinical or chronic stress on the involved intervertebral disc.     

单纯切除及联合自体角膜缘干细胞移植治疗翼状胬肉83例疗效观察

目的探讨翼状胬肉切除联合自体角膜缘干细胞移植治疗翼状胬肉的疗效。方法翼状胬肉83例（89眼）,采用单纯翼状胬肉切除术及联合自体角膜缘干细胞移植两种方法治疗,比较两种术式的术后复发率。结果按Prabhasawat等提出的翼状胬肉术后疗效分级标准,单纯翼状胬肉切除组术32例（33眼）,出现3级5例,4级即真性复发4例;自体角膜缘干细胞移植组51例（56眼）,出现2例3级,没有出现4级。结论自体角膜缘干细胞移植组术后复发率明显降低,方法简单易行,取自体角膜缘移植片方便,排斥反应小,是目前治疗翼状胬肉有效的方法之一。