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1.
采用流变学法测定了亚麻籽胶溶液的胶凝点、熔化点,并采用质构仪、扫描电镜和原子力显微镜等手段研究了影响亚麻籽胶凝胶强度的因素,结果表明亚麻籽胶具有胶凝性,它能形成一种热可逆的冷致凝胶,亚麻籽胶溶液的胶凝点低于其凝胶的熔化点,且亚麻籽胶溶液的胶凝点及其凝胶的熔化点均随冷却的起始温度的升高而升高。亚麻籽胶浓度、溶解温度、pH、NaCl、CaCl2及复合磷酸盐能影响亚麻籽胶的凝胶强度,亚麻籽胶的凝胶强度随着浓度的增加及溶解温度的升高而增强;在pH6~9的范围内,亚麻籽胶的凝胶强度达到最大;NaCl和复合磷酸盐可以降低亚麻籽胶的凝胶强度,低浓度(〈0.3%)的CaCl2可以增强亚麻籽胶的凝胶强度,而高质量分数(〉0.3%)的CaCl2能降低亚麻籽胶的凝胶强度。 相似文献
2.
目的:观察蛙皮素(BBS)对前列腺癌PC-3细胞骨架形态及细胞内游离Ca2+([Ca2+]i)浓度的影响。方法:①利用免疫荧光法(IH),结合激光扫描共聚焦显微镜(LSCM)检测10-5mol/L浓度BBS处理的PC-3细胞角蛋白(CK)的表达,以反映其对细胞骨架形态的影响;②应用Fluo-3/AM荧光标记技术和LSCM检测不同浓度BBS(10-9、10-7、10-5mol/L)处理的PC-3细胞[Ca2+]i浓度。结果:①10-5mol/L浓度的BBS可促进PC-3细胞CK表达及伪足形成;②BBS可提高PC-3细胞[Ca2+]i浓度,并具有浓度依赖性。结论:实验证明一定浓度的BBS可明显提高PC-3细胞[Ca2+]i浓度及CK表达,进而影响PC-3细胞骨架形态。本研究为探索BBS应用于肿瘤研究以及BBS作用后细胞内信息传递途径提供了基础。 相似文献
3.
强脉冲光治疗浅表(皮肤)血管瘤临床观察 总被引:4,自引:4,他引:0
目的:观察强脉冲光(IPL)治疗浅表(皮肤)血管瘤的疗效。方法:选择60例浅表(皮肤)血管瘤患者,随机分为治疗组和对照组,治疗组用535nm、580nm强脉冲光治疗皮损,每三周治疗一次,三次为一个疗程;对照组采用波长为532nm的Q开关Nd:YAG激光治疗,每两周治疗一次,三次为一个疗程。观察两组皮损的治愈率、并发症等情况,并行统计学处理。结果:治疗组皮损三次治愈率明显高于对照组(P〈0.01),患处瘢痕形成及炎症性色素沉着发生率均低于对照组。结论:强脉冲光(IPLTM)治疗浅表(皮肤)血管瘤效果满意。 相似文献
4.
5.
目的比较研究成年大鼠细胞周期蛋白依赖性激酶抑制因子在神经元和星形胶质细胞的表达差异。方法应用免疫荧光和激光扫描共聚焦显微镜观察成年大鼠生理状态下大脑皮层或海马CA1、CA3、DG区神经元和星形胶质细胞细胞周期蛋白依赖性激酶抑制因子(CDKI)p15Ink4b、p21cipl的表达。结果成年大鼠海马区和大脑皮层的神经元有p15Ink4b和p21cipl的表达,细胞核和细胞浆均有表达,且以胞核为主;星形胶质细胞也有上述细胞周期调控蛋白的表达,便细胞数目较少,并且表达这些指标的星形胶质细胞多聚集在海马区。结论成年大鼠大脑皮层和海马区的神经元和星形胶质细胞均表达p15Ink4b和p21cipl,而其在神经元的表达较星形胶质细胞更为普遍。 相似文献
6.
Chun-Hsiung Huang Fang-Yuan Ho Hon-Ming Ma Chan-Tsung Yang Jiann-Jong Liau Hung-Chan Kao Tai-Horng Young Cheng-Kung Cheng 《Journal of orthopaedic research》2002,20(5):1038-1041
Osteolysis induced by ultrahigh molecular weight polyethylene wear debris has been recognized as the major cause of long-term failure in total joint arthroplasties. In a previous study, the prevalence of intraoperatively identified osteolysis during primary revision surgery was much higher in mobile bearing knee replacements (47%) than in fixed bearing knee replacements (13%). We postulated that mobile bearing knee implants tend to produce smaller sized particles. In our current study, we compared the particle size and morphology of polyethylene wear debris between failed mobile bearing and fixed bearing knees. Tissue specimens from interfacial and lytic regions were extracted during revision surgery of 10 mobile bearing knees (all of the low contact stress (LCS) design) and 17 fixed bearing knees (10 of the porous-coated anatomic (PCA) and 7 of the Miller/Galante design). Polyethylene particles were isolated from the tissue specimens and examined using both scanning electron microscopy and light-scattering analyses. The LCS mobile bearing knees produced smaller particulate debris (mean equivalent spherical diameter: 0.58 microm in LCS, 1.17 microm in PCA and 5.23 microm in M/G) and more granular debris (mean value: 93% in LCS, 77% in PCA and 15% in M/G). 相似文献
7.
目的:了解抗龋IgY和IgY牙膏对体外培养细胞的影响。方法:体外培养人成纤维细胞(human skin fibrobasts,HSF)和中国仓鼠卵巢癌细胞(chinese hamster ovary,CHO),分别经不同浓度的抗龋IgY和IgY牙膏处理后常规培养,通过倒置显微镜和电子显微镜观察细胞生长情况和细胞超微结构。结果:经抗龋IgY处理的HSF和CHO细胞生长旺盛,超微结构无变化;经抗龋IgY牙膏处理的HSF和CHO细胞加药后部分细胞出现变圆或脱落、细胞生长缓慢和细胞器变性等变化,第2天恢复良好生长状态。结论:抗龋IgY对体外培养细胞生长、形态无影响;抗龋IgY牙膏中的某些成分可能对培养细胞有短暂的不良影响。 相似文献
8.
Summary Patients with post-concussional symptoms (PCS) about 6 months after a mild head injury (MHI) were examined for tolerance of light and sound in comparison with concussed patients without PCS and nonconcussed healthy controls. MHI patients with PCS were individually matched with subjects from the two control groups for the time elapsed from the injury, and for age and sex. Using a computerized rating technique, we assessed both the maximal and submaximal levels of lowered tolerance for light and sound over a wide range of stimuli. We found that the MHI patients with PCS 6 months after the trauma (n=11) tolerated significantly less well stimuli of intensities of 71 dB and 500 lx than MHI patients without PCS (n=11) and non-concussed controls (n=11). There were no significant differences in tolerance for light and sound between MHI patients without PCS and the non-injured controls. Decreased tolerance for light and sound may contribute to the persistence of symptoms up to 6 months after a mild head injury. The psychophysical method provides an objective measure for the evaluation of the late persistent postconcussional syndrome. 相似文献
9.
We describe a case of elastosis perforans serpiginosa with additional findings of degenerative skin changes. A 20-year-old man with hepatolenticular degeneration, under prolonged treatment with D-penicillamine, presented with a circular or serpiginous arrangement of nuchal papules. Histopathologically, transepidermal channels were accompanied by granulomatous reactions, with several giant cells engulfing elastic fibers. In addition to these findings of a typical elastosis perforans serpiginosa, we observed scar-like skin changes inside the circular arrangement of the papules. At the scar-like tissue, we found electron-microscopical evidence of randomly aggregated thin collagen fibers with no tendency toward systemic combined bundle formation, which is a characteristic feature of normal collagen fiber formation. Pseudoxanthoma-elasticum-like changes were observed on his neck. On his axillae and groin, slight skin thickening and wrinkling were detected. The diagnosis of elastosis perforans serpiginosa does not represent all of the manifestations or the pathological background described above. The skin manifestations described here represent not only an elastosis but also a total degenerative dermatosis with over-healed collagenosis. Thus, those dermatoses should be summarized as one entity, penicillamine-induced degenerative dermatosis. After considering the pathogenic background and clinical similarities, we further propose to simplify the penicillamine-induced skin manifestations to three categories: acute sensitivity reactions, bullous dermatoses, and degenerative dermatoses. 相似文献
10.