白介素-6对前列腺癌细胞生长的作用

目的：观察IL-6对前列腺癌细胞生长的影响，对雄激素依赖型和雄激素非依赖型前列腺癌细胞作用的差别。方法：分别测定雄激素依赖的LNCaP细胞和雄激素非依赖的PC-3细胞产生IL-6的能力及外源性IL-6和抗IL-6抗体对此两种细胞生长的影响。结果：LNCaP细胞几乎不产生IL-6，1ml(10^6个)PC-3细胞平均产生IL-6190pg/48h；外源性IL-6对LNCaP细胞生长有明显抑制作用，但对PC-3细胞的生长无明显作用；抗IL-6抗体能明显抑制PC-3细胞的生长，对LNCaP细胞生长无影响。结论：PC-3细胞以自分泌的形式分泌大量IL-6，抗IL-6抗体的封闭作用可抑制PC-3细胞的生长，外源性IL-6能抑制LNCaP细胞的生长。     

奥硝唑对滴虫性阴道炎治疗作用的临床观察

目的探讨奥硝唑治疗滴虫性阴道炎的疗效,关注治疗前后阴道灌洗液中白细胞介素-6（IL-6）的表达差异。方法190例滴虫性阴道炎患者依入院顺序分为两组。对照组共95例,应用替硝唑进行治疗;观察组共95例,应用奥硝唑治疗。观察治疗的效果,同时检测治疗前、治疗后阴道灌液液中IL-6的含量,观察不同治疗方法对IL-6的影响。结果治疗5d后,观察组的总有效率明显高于对照组,观察组与对照组患者治疗后阴道灌洗液中的IL-6均下降,但是观察组的下降值明显高于对照组。结论滴虫性阴道炎患者应用奥硝唑治疗临床疗效明显,并能有效调节阴道中的IL-6的含量,进而调节阴道的微环境,临床治疗中可以推广应用。     

白介素-2对胃癌术后患者免疫功能影响

目的：通过观察胃癌术后病人白介素－2（IL－2）治疗过程中外周血淋巴细胞表型、血清白介素－2受体（SIL－2R）和血清白介素－12（IL－12）水平的变化，对胃癌术后患者的免疫机能的影响进行评价。方法：胃癌术后患者64例随机分成两组：一组给予IL－2，另一组为对照组。用药前、后分别行IL－12和SIL－2R测定以及用流式细胞仪测定淋巴细胞的表型。结果：用药后血清IL－12及CD4^ 、CD8^ 、CD56^ 比用药前明显升高（P＜0．05）。而SIL－2R明显下降（P＜0．05）。结论：应用IL－2治疗胃癌术后患者，其细胞免疫机能明显改善。     

血清促炎细胞因子水平与急性胰腺炎严重程度的相关性研究

目的 探究血清促炎细胞因子白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)及高迁移率族蛋白B-1(HMGB1)水平在早期评估急性胰腺炎(AP)患者严重程度及预后的价值.方法 以33例重症急性胰腺炎(SAP)和38例轻症急性胰腺炎(MAP)患者为研究对象,另以51名健康体检者作为对照组.采用酶联免疫吸附试验(ELISA)检测血清IL-6、TNF-α及HMGB1水平,分析三者与患者Ranson评分、APACHEⅡ评分、Bahhazar CT评分、血清生化指标及预后的关系.结果 SAP组、MAP组、健康对照组血清IL-6水平分别为(553.72±175.76)pg/ml、(265.73±179.95)pg/ml和(16.43±3.32)pg/ml,三组间差异均有统计学意义(P均＜0.01).SAP组、MAP组、健康对照组血清TNF-α水平三组间差异均无统计学意义(P均＞0.05).SAP组、MAP组、健康对照组血清HMGB1水平分别为(11.48±6.94)μg/L、(6.13±5.80)μg/和(1.82±0.64)/μg/L,三组间HMGB1值差异均有统计学意义(P均＜0.05).患者血清HMGB1水平与IL-6,TNF-α的相关系数分别为0.896和0.724(P＜0.01).血清IL-6水平与Ranson评分、APACHE Ⅱ评分、Balthazar CT 评分均呈正相关;血清TNF-α水平与APACHEⅡ评分呈正相关;血清HMGB1水平与Ranson评分、Balthazar CT评分呈正相关.三者血清水平均与肌酐值呈正相关.病程中出现局部和(或)全身并发症者血清IL-6水平显著高于无并发症者.结论 血清IL-6、TNF-α、HMGB1水平与胰腺炎病情的严重程度显著相关,三者参与AP时急性肾功能不全的发生,血清IL-6水平升高与并发症发生显著相关.

Abstract：

Objective To explore the value of serum level of interleukin 6 (IL-6),tumor necrosis factor alpha (TNF-α) and high mobility group box-1 protein( HMGB1) in early assessment the severity and prognosis of acute pancreatitis (AP). Methods Thirty-three severe acute pancreatitis (SAP) patients and 38 mild acute pancreatitis (MAP) patients were selected as study objects;and 51healthy individuals were set as control group. Serum IL-6,TNF-α and HMGB1 concentrations were determined by enzyme-linked immunosorbent assay (ELISA),the association of them and the scores of Ranson,APACHE Ⅱ . Balthazar CT,serum biochemical parameters and prognosis was analyzed.Results The serum IL-6 levels of SAP group,MAP group and healthy control group were (553. 72±175.76) pg/ml,(265. 73±179. 95) pg/ml and (16. 43±3. 32) pg/ml;and there were statistical significance of these three groups (all P＜0. 01). There was no significant difference of TNF-α in the three groups (all P＞0. 05). The serum HMGB1 levels of SAP group,MAP group and healthy control group were (11. 48±6. 94)μg/L,(6. 13±5. 80)μg/L and (1. 82±0. 64)μg/L respectively,and there were statistical significant of these three groups (all P＜0. 05). The correlation coefficient of serum HMGB1 with IL-6 and TNF-α were 0. 896 and 0. 724 (P＜0. 01) respectively. The IL-6 level was positively correlated with the scores of Ranson,APACHE Ⅱ and Balthazar CT. The TNF-αconcentration was positively correlated with APACHE Ⅱ score,and the HMGB1 concentration positively correlated with scores of Ranson and Balthazar CT. The levels of IL-6,TNF-α and HMGB1were all positively correlated with the serum creatinine concentration. The IL-6 levels of patients with local and/or systemic complications were significantly higher than those without complications.Conclusion The serum levels of IL-6,TNF-α and HMGB1 are significantly correlated with the severity of pancreatitis,all of them take part in the development of acute renal insufficiency. The high level of serum IL-6 significantly correlated with complications.     

γ射线全身照射后小鼠骨髓造血细胞IL—3基因表达的 …

目的 探讨内源性ＩＬ－３基因表达在骨髓辐射损伤后修复中的作用。方法 ５．５Ｇｙ＾６０Ｃｏγ射线全身照射７８只ＬＡＣＡ小鼠，于照射后４周内分批活杀，将骨髓进行ＨＥ染色，并进行内源性ＩＬ－３基因表达的免疫组化、原位杂交和原位反转录ＰＣＲ检测。结果 照射后４周内小鼠骨髓出现明显损伤及损伤后重建现象。免疫组化观察ＩＬ－３于修复期造血细胞浆内明显增多，尤以照后２１天其量达高峰，而高位杂交则显示其ｍＲＮＡ于照