染料木黄酮对高同型半胱氨酸诱导的人血管内皮细胞组织因子表达的干预作用

目的探讨染料木黄酮对高同型半胱氨酸诱导的人大隐静脉内皮细胞表达组织因子(TF)的干预作用。方法不同浓度(0,5,10,20,40,50mg/L)染料木黄酮,不同时间(2、4、8、12、24、48、72h)作用于高同型半胱氨酸下(50μmol/L)的人大隐静脉内皮细胞,采用反转录-聚合酶链反应(RT-PCR)和酶联免疫吸附试验(ELISA)法检测TFmRNA表达和TF蛋白浓度。结果作用时间一定时间(24h),随着染料木黄酮浓度的增加,同型半胱氨酸诱导的内皮细胞培养液中TF蛋白浓度及内皮细胞TFmRNA表达水平显著降低(r=0.994,0.992,P<0.05);在染料木黄酮浓度一定(40mg/L)时,随着作用时间的延长,同型半胱氨酸所诱导的内皮细胞培养液中TF蛋白浓度及内皮细胞TFmRNA表达水平显著降低(r=0.972,0.982,P<0.05)。结论染料木黄酮一定程度上可抑制高同型半胱氨酸所致的内皮细胞TF表达的上调,抑制作用具有剂量和时间依赖性。     

The presence of monoiodinated derivates of daidzein and genistein in human urine and its effect on thyroid gland function

Dietary supplements used by women during menopause are usually based on plant extracts containing isoflavonoids, daidzein and genistein. Genistein is a known inhibitor of many enzymes, including thyroid peroxidase (TPO). In the thyroid follicle, genistein acts as its alternate substrate for the formation of genistein iodinated derivatives. The aim of this study was to search for daidzein- and genistein-iodinated derivatives in urine of isoflavonoid-supplemented women. Additionally, selected phytoestrogens, steroid and thyroid hormones before and after three months of phytoestrogen supplementation were estimated. Urinary levels of free phytoestrogen increased significantly after therapy. They ranged between 0.3–1600, 0.6–670 and 0–206 nmol/L for daidzein, genistein and S-equol, respectively. Monoiodinated derivatives of genistein were observed (0–504 pmol/L) in 60% of the investigated samples. Steroid and thyroid hormone levels were within the normal range and were not significantly altered. The presence of monoiodinated derivates in human urine confirmed that genistein and daidzein may enter human thyroid follicles and influence TPO. Since the levels of the free thyroid hormones were not affected, we propose that the use of phytoestrogen dietary supplements is not associated with the development of thyroid-gland disorders in subjects with adequate iodine intake.     

金雀异黄酮的抗血小板聚集作用及机制

目的观察金雀异黄酮对血小板聚集的影响,并初步探讨其作用机制。方法制备家兔富血小板血浆和贫血小板血浆,并通过加入不同浓度的金雀异黄酮,测定其对凝血酶和血小板活化因子(PAF)诱导的血小板聚集的抑制作用;并测定其对凝血酶的抑制率及PAF诱导聚集下的血栓素(TXB2)、6-酮-前列腺-F1α(6-Keto-PGF1α)的水平。结果金雀异黄酮能明显抑制由凝血酶和PAF诱导的血小板聚集,且金雀异黄酮与凝血酶有一定的亲和力,在PAF诱导的血小板聚集中,金雀异黄酮能明显降低TXB2水平,升高6-Keto-PGF1α水平。结论金雀异黄酮能够明显抑制由凝血酶和PAF诱导的血小板聚集,其作用与抑制凝血酶有一定关系外,可能与其通过调节PAF诱导聚集TXA2和PGI2的释放有关。     

金雀异黄酮通过调控转胶蛋白-2表达对缺氧/复氧诱导的心肌细胞损伤的影响

目的 研究金雀异黄酮(GEN)对缺氧/复氧诱导的心肌细胞损伤的影响及可能分子机制.方法 本研究的起止时间为2018年10月至2019年11月.建立心肌细胞缺氧/复氧(H/R)模型(H/R组),以常规培养的细胞作为对照组;用浓度分别为5 mg/L、10 mg/L、20 mg/L的GEN处理H9c2细胞后再进行H/R处理,...     

Genistein对APP695转染PC12细胞凋亡的保护作用

目的观察Genisteint（GST）对APP695转染PCI2细胞凋亡的保护作用。方法将培养的PC12细胞分为正常对照组、APP695转染组、GST干预组。采用流式细胞术检测PC12细胞凋亡率。结果APP695转染组与正常对照组比较,PC12细胞存活率明显降低,凋亡率明显升高（P〈0,01）。GST干预组与APP695转染组比较,细胞存活率显著升高,凋亡率显著降低,其中1μmol／LGST组与APP695转染纽此较,细胞凋亡率降低（P〈0．05）,5μmol／LGST组较APP695转染组细胞凋亡率则明显降低（P〈0．01）。结论GST对APP695基因转染引起的PC12细胞损伤有一定的保护作用。     

三羟基异黄酮对肝癌细胞侵袭转移能力的影响

目的探讨三羟基异黄酮（Genistein）对肝癌细胞侵袭转移能力的影响及其机制.方法以人高转移肝细胞癌细胞系HCCLM3为研究对象,用细胞基质粘附实验检测Genistein对肝癌细胞基质粘附能力的影响,Transwell小室侵袭运动实验检测Genistein对肝癌细胞侵袭、运动能力的影响.免疫细胞化学法检测肝癌细胞MMP-2蛋白表达.结果 Genistein能抑制肝癌细胞对FN和Marigel胶的粘附;Genistein能抑制肝癌细胞在Transwell小室中的侵袭运动;Genistein处理组肝癌细胞MMP-2蛋白表达较对照组降低.结论 Genistein能抑制肝癌细胞的侵袭转移,其机制之一可能在于Genistein能抑制肝癌细胞MMP-2蛋白表达.     

染料木素铬配合物对谷氨酸钠诱导的肥胖大鼠糖脂代谢的影响

目的:通过研究染料木素铬配合物(染料木素铬)对L-谷氨酸钠(MSG)诱导的肥胖大鼠糖代谢和脂代谢的影响,为开发治疗肥胖型2型糖尿病的新药提供依据。方法:采用MSG制备肥胖大鼠模型,将成模后的雌性大鼠(体质量超过对照组大鼠平均体质量的20%) 50只随机为模型组、二甲双胍(95 mg·kg^-1)组、低剂量(15 mg·kg^-1)染料木素铬组、中剂量(30 mg·kg^-1)染料木素铬组和高剂量(60 mg·kg^-1)染料木素铬组,另取10只正常大鼠设为对照组,按上述剂量灌胃给予受试药物,每日1次,连续4周。给药结束后取静脉血,采用氧化酶法检测大鼠血清中血糖(Glu)、甘油三脂(TG)和总胆固醇(TC)水平,酶联免疫吸附测定(ELISA)法检测大鼠血清中糖化血红蛋白(GHb)、胰岛素(INS)、一氧化氮合酶(NOS)、血管紧张素Ⅱ(AngⅡ)、内皮因子1 (ET-1)、前列环素2 (PGI2)、肿瘤坏死因子α (TNF-α)和白细胞介素6 (IL-6)水平。结果:与对照组比较,给药前后其余各组大鼠体质量明...     

Comparative effect of genistein and daidzein on the expression of MCP-1, eNOS, and cell adhesion molecules in TNF-��-stimulated HUVECs

We compared the effects of genistein and daidzein on the expression of chemokines, cell adhesion molecules (CAMs), and endothelial nitric oxide synthase (eNOS) in tumor necrosis factor (TNF)-α-stimulated human umbilical vascular endothelial cells (HUVECs). TNF-α exposure significantly increased expression of monocyte chemoattractant protein (MCP)-1, vascular adhesion molecule (VCAM)-1, and intercellular adhesion molecule-1. Genistein significantly decreased MCP-1 and VCAM-1 production in a dose-dependent manner, whereas CAM expression was not significantly lowered by genistein treatment. However, daidzein slightly decreased MCP-1 production. The effects of genistein and daidzein on MCP-1 secretion coincided with mRNA expression. Pre-treatment with either genistein or daidzein elevated eNOS expression and nitric oxide production disturbed by TNF-α exposure. A low concentration of isoflavones significantly inhibited nuclear factor (NF)κB activation, whereas a high dose slightly ameliorated these inhibitive effects. These results suggest that genistein had a stronger effect on MCP-1 and eNOS expression than that of daidzein. Additionally, NFκB transactivation might be partially related to the down-regulation of these mRNAs in TNF-α-stimulated HUVECs.     

金雀异黄酮对去卵巢大鼠脑抗氧化作用的影响

目的研究金雀异黄酮对去卵巢大鼠大脑抗氧化作用的影响,为使用植物雌激素防治女性更年期后老年性痴呆提供依据。方法40只3月龄雌性SD大鼠,随机分为假手术组、去卵巢对照组、金雀异黄酮组和苯甲酸雌二醇组,给予相应手术和治疗。术后6周分析大鼠额叶、颞叶、海马和基底前脑的SOD和MDA值。结果假手术组颞叶和海马的SOD值低于基底前脑(P<0.05);额叶和颞叶的MDA值高于海马和基底前脑(P<0.05);去卵巢对照组基底前脑的SOD值显著低于假手术组、金雀异黄酮组和苯甲酸雌二醇组(P<0.05),MDA值显著高于假手术组、金雀异黄酮组和苯甲酸雌二醇组(P<0.05);假手术组、金雀异黄酮组及苯甲酸雌二醇组之间SOD值和MDA值差异无统计学意义(P>0.05)。结论去卵巢对照组大鼠基底前脑抗氧化能力显著降低,氧化损伤程度显著升高,金雀异黄酮替代治疗可增加去卵巢大鼠基底前脑的抗氧化能力,降低基底前脑的氧化损伤,可用于女性更年期后老年性痴呆的防治。     

染料木素磺酸钠对离体心脏缺血再灌注损伤的保护作用

目的：研究染料木素磺酸钠（GSS）对离体心脏缺血再灌注损伤的保护作用。方法：应用大鼠离体心肌缺血再灌注损伤模型,在灌流液中加入高、中、低剂量的染料木素磺酸钠。测定大鼠心率、左心室收缩压、心肌舒张/收缩速度及心肌组织乳酸脱氢酶（LDH）和心肌肌酸激酶（CK）活性的变化。结果：给药组与模型组相比,缺血再灌后染料木素磺酸钠组心率减慢、左心室收缩压升高、心肌舒张/收缩功能明显改善;心肌LDH及CK的活性更高（P〈0.01或P〈0.001）。结论：染料木素磺酸钠对离体心脏缺血再灌注损伤起保护作用。