急性脑出血性疾病与血浆过氧化脂质、超氧化物岐化酶、谷胱甘肽过氧化物酶关系的研究

目的：探讨急性脑出血性疾病与血浆过氧化脂质（ＬＰＯ）、超氧化物岐化酶（ＳＯＤ）、谷胱甘肽过氧化物酶（ＧＳＨ－ＰＸ）的关系。方法：用改良硫代巴比妥法、黄嘌呤氧化酶法和谷胱甘肽氧化酶法对４４例急性颅内出血患者血浆ＬＰＯ,ＳＯＤ和ＧＳＨ－ＰＸ含量测定,并以２６例正常对照。结果：急性脑出血患者血浆ＬＰＯ显著高于正常对照（Ｐ＜０．００１）,而ＳＯＤ、ＧＳＨ－ＰＸ显著低于正常人组（Ｐ＜０．００１、Ｐ＜０．０５）。ＬＰＯ与ＳＯＤ、ＧＳＨ－ＰＸ呈负相关（ｒ＝－０．２８,ｒ＝－０．３８）。结论：血浆中ＬＰＯ、ＳＯＤ、ＧＳＨ－ＰＸ含量测定对判断急性脑出血性疾病严重程度、评估预后及辅助治疗有一定参与价值。     

原发性肾病患儿血GSHPX／LPO比值的探讨

本文观察了40例2－13岁原发性肾病患儿血GSHPX／LPO经值变化，对照组为50例体检健康儿童，结果表明：肾病组血GSHPX／LPO比值较对照组极显著降低（P＜0．01），本病确实存在氧化与抗氧化平衡失调，为肾病的抗氧化治疗提供了又一客观理论基础。     

活动性肺结核病患者血中SOD,GSHPX,LPO的动态变化

对２０例活动性肺结核病人血中超氧化物歧化酶（ＳＯＤ）、谷胱甘肽过氧化物酶（ＧＳＨＰＸ）、过氧化脂质（ＬＰＯ）的动态观察发现：活动性肺结核病人血中ＳＯＤ、ＧＳＨＰＸ的水平明显低于正常人，而ＬＰＯ则高于正常人（Ｐ＜０．０１）。经正规抗痨治疗１个月后，再次检测上述指标，发现ＳＯＤ、ＧＳＨＰＸ均有不同程度升高（分别增加１７．４％和６．９％），而ＬＰＯ则下降２１．９％（Ｐ＜０．０５）；且三项指标与正常组对比仍存在显著性差异（Ｐ＜０．０５）。提示：活动性肺结核的病情与血中ＳＯＤ、ＧＳＨＰＸ、ＬＰＯ的水平有密切的关系。这些指标对活动性肺结核的病情分析、疗效观察及预后有一定的实用价值。     

Modulation of apoptosis and improved redox metabolism with the use of a new antioxidant formula

Oxidative stress is involved in the pathogenesis of a wide spectrum of diseases, implicating that strategies directed at counterbalancing oxidative processes could have a role in clinical medicine. There is also an evidence that oxidative stress acts as a major determinant of apoptotic cell death. Many studies have reported favourable effects of antioxidant formulas on several parameters of the oxidant-antioxidant balance, but none of them has focused whether antioxidant formulas could modulate apoptosis. We investigated in 20 healthy individuals the effect of supplementation with a formula containing alpha-tocopherol, alpha-lipoic acid, coenzyme Q(10), carnitines, and selenomethionine, on plasma oxidant status and peroxide levels, erythrocyte antioxidant enzymes, lymphocyte apoptosis, and generation of ROS at the mitochondrial level. Control subjects received only carnitines or an incomplete formula with alpha-tocopherol, alpha-lipoic acid, coenzyme Q(10), and selenomethionine. Supplementation with the complete formula resulted in a significant increase in the plasma antioxidant status that was mirrored by a decrease in blood peroxide levels and a reduced generation of ROS at the mitochondrial level. This was associated with a significant decrease in the frequency of peripheral blood lymphocytes, with either CD4 or CD8 phenotype, undergoing apoptosis. Less consistent results were found when either incomplete formula was used. Our study suggests that supplementation with antioxidant formulas can modulate the process of apoptosis under in vivo conditions. The clinical potential of this strategy in the treatment of diseases with an elevated commitment to apoptosis should be explored.