We evaluated the inhibitory effect of DS-4574, a peptidoleukotriene antagonist with mast cell stabilizing action, on rat gastric mucosal lesions induced by compound 48/80 (C48/80: a mast cell degranulator), in comparison with those of disodium cromoglycate (DSCG: a mast cell stabilizer), LY171883 (a peptidoleukotriene antagonist) and cimetidine (a histamine H2 receptor antagonist). Subcutaneous administration of C48/80 (1 mg/kg) once daily for four consecutive days produced extensive gastric lesions in the fundic mucosa. DS-4574 (20, 50 and 100 mg/kg/day, oral) and DSCG (200 mg/kg/day, intraperitoneal) treatment markedly inhibited formation of these mucosal lesions, but LY171883 (100 and 200 mg/kg/day, oral) and cimetidine (400 mg/kg/day, oral) treatment did not. Moreover, DS-4574 and DSCG significantly suppressed both hyperhistaminemia and histamine release from rat peritoneal mast cells induced by C48/80. These results indicate that the inhibitory effect of DS-4574 on gastric lesions induced by C48/80 may be related to its mast cell stabilizing action, but to neither its antisecretory nor its peptidoleukotriene antagonistic activity. 相似文献
Cementum resorption (cementolysis) comparable to osteocytic osteolysis and concomitant with cementocyte maturation has been observed in alpharadiographs of rat molars. This phenomenon was enhanced by administration of parathyroid extract (Para-Thormone, Lilly) and retarded by feeding a diet containing 1% MgO for 48 days.
3H-thymidine-labeled rats have shown cells with radioactive nuclei at the cementodentinal junction 3 days later and none thereafter, pointing to a turnover of young cementum comparable to that of trabecular bone.
Zusammenfassung Eine Resorption des Zements (Zementolyse), vergleichbar mit einer durch Osteocyten verursachte Osteolyse, und begleitet von einer Zementocytenreifung wurde anhand von Alpha-Radiographien von Rattenbackenzähnen beobachtet.Dieser Vorgang wurde durch Verabreichung von Parathyroid-Extrakt (Para-Thormone, Lilly) gesteigert und durch 48tägige Verfütterung einer 1% MgO enthaltenden Diät verlangsamt.Ratten, die mit3H-Thymidin markiert sind, zeigten Zellen mit radioaktivem Nuclei an der zemento-dentinalen Übergangsstelle, und zwar nur nach 3 Tagen — später nicht mehr. Dies weist auf einen Umsatz von jungem Zement hin, ähnlich jenem der Knochenbälkchen.
Résumé L'existence d'un phénomène de résorption comparable à l'ostéolyse péri-ostéocytaire au niveau des cementocytes adultes du rat a été mise en évidence par l'alpharadiographie. Cette «cémentolyse» a été stimulée par l'administration d'extrait parathyroîdien et retardée chez des rats dont la diète contenait 1% de MgO.La thymidine tritiée a été retrouvée par radioautographie dans des noyaux de cellules situées à la jonction cément-dentine, 3 jours aprés l'injection, démontrant ainsi un «turnover» du cément des jeunes rats, comparable à celui de l'os trabéculaire.
Trastuzumab deruxtecan (DS-8201a) is an antibody-drug conjugate (ADC) composed of a monoclonal antibody targeting human epidermal growth factor receptor 2 (HER2) conjugated to a topoisomerase I inhibitor (DXd) at a drug-to-antibody ratio (DAR) of 7-8. Here, we examined the pharmacokinetic (PK) profiles of DS-8201a and DXd in cynomolgus monkeys, a cross-reactive species.
Following intravenous (iv) administration of DS-8201a, the linker was stable in plasma, and systemic DXd exposure was low. DXd was rapidly cleared following iv dosing. Biodistribution studies revealed that intact DS-8201a was present mostly in the blood without tissue-specific retention. The major pathway of excretion for DXd was the faecal route following iv administration of radiolabelled DS-8201a. The only detectable metabolite in the urine and faeces was unmetabolized DXd. DXd is a substrate of organic anion transporting polypeptides, P-gp, and breast cancer resistance protein.
In conclusion, the stable linker in circulation and the high clearance of DXd upon release resulted in the low systemic exposure to DXd. Furthermore, the minimal tissue-specific retention and rapid excretion of DXd into faeces as its unmetabolized form with potentially limited impact on drug???drug interaction as a victim were also critical elements of the PK profile of DS-8201a.
The antisecretory effect of DS-4574, a mast cell stabilizer with peptidoleukotriene antagonism, on the hypersecretion of gastric acid stimulated by several secretagogues was examined in the pig. Goettingen miniature pigs with chronic gastric fistula were used. Intramuscular injection of carbachol (60 g/kg), tetragastrin (50 g/kg) or histamine (200 g/kg)-induced gastric acid hypersecretion. Intraduodenal administration of DS-4574 (10 and 20 mg/kg) significantly inhibited both the hypersecretion induced by carbachol and that by tetragastrin. On the other hand, DS-4574 (50 mg/kg, intraduodenal) did not suppress histamine-induced hypersecretion. In thein vitro study, no effect on hog gastric K+-dependent ATPase activity was found at concentrations of DS-4574 from 10–7 to 10–4M. These results were highly similar to those in the rat. The suppression of histamine release from histamine-containing cells in the gastric mucosa of the rat was concluded to be an antisecretory effect of DS-4574. 相似文献