Hepatoprotective activity of the ethanol extract of Sarcopyramis Nepalensis

The present study examined the protective effect of the ethanol extract of Sarcopyramis nepalensis (EESN) on agents-induced hepatotoxicity in mice and the possible mechanism. Acute liver injury was induced by administration of either CCl4 or D-GalN. The animals were divided into 5 groups in terms of different treatment: normal group, CCl4 or D-GalN group, silymarin or bifendate group, low dose EESN group (10 mg/kg) and high dose EESN group (30 mg/kg). Liver function was evaluated by detecting the levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The oxidize stress markers were measured, including malondialdehyde (MDA), glutathione peroxidase (GSH) and superoxide dismutase (SOD). Liver tissues were histopathologically examined by hematoxy-lin-eosin (H&E) staining. The acute toxicity study revealed that there was no toxicity of EESN at the dose of 5 g/kg in mice. The levels of ALT and AST in serum, and the MDA level in live tissues were significantly increased and the activities of SOD and GSH substantially decreased in mice after CCl4 or D-GalN treatment. These biochemical and oxidize stress markers were profoundly improved after treatment with EESN at different doses, which was similar to the results of silymarin or bifendate treatment. The histophathological examination revealed the significant improvement in the pathological changes of the liver in EESN-treated mice as compared to those in CCl4 or D-GalN group. It was concluded that EESN possesses potential antioxidant and hepatoprotective properties and has therapeutic potential for liver diseases.     

银杏叶片对化学诱导急性肝坏死大鼠的作用

目的　观察银杏制剂对急性肝损害大鼠的保护作用。方法　腹腔内注射 10 %D 氨基半乳糖致动物急性肝损伤 ,分为模型组、预防 1和 2组 (给银杏制剂 )及阳性对照组 (给齐墩果酸片 ) ,另有空白对照组及给等量的蒸馏水灌胃 ,1天 1次 ,共 7天。末次给药后 ,禁食 2 4h后断头取血及留肝组织作病理检查。结果　模型组大鼠血中PAF和ALT明显升高 ,病理肝脏细胞明显变性坏死 ,而预防组大鼠各项指标明显改善 ,病理损害程度也明显减轻。结论　银杏内酯能部分阻断PAF对肝脏的损害 ,具有一定的保肝作用     

胡黄连提取物对四氯化碳或半乳糖胺损伤的原代培养大鼠肝实质细胞的作用

为研究胡黄连提取物对四氯化碳(CCI4）或半乳糖胺(D-GaIN）损伤原代培养大鼠肝实质细胞的保护作用。半原位酶分离法分离大鼠肝细胞。利用CC44或D-GaIN引起原代培养大鼠肝实质细胞损伤模型。以培养液中谷丙转氨酶(ALT）活性变化为指标。并采用MTT法对细胞活性和增殖进行检测。结果：胡黄连提取物能够增强受损肝细胞的活性和增殖能力。但不能显著降低CCI4或D-GaIN肝损伤模型ALT活性。     

丹栀逍遥散对D-氨基半乳糖所致大鼠急性肝损伤保护作用的研究

目的探讨丹栀逍遥散对D-氨基半乳糖(D-GaIN)所致大鼠急性肝损伤的保护作用机制。方法将60只SD雄性大鼠随机分为空白组,模型组,联苯双酯组,丹栀逍遥散高、中、低剂量组,每组10只。连续灌胃给药7 d后,除空白组腹腔注射等体积生理盐水外,其余大鼠均腹腔注射D-GaIN。24 h后,检测血清中天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、肝匀浆中超氧化物歧化酶(SOD)、还原型谷胱甘肽-过氧化物酶(GSH-PX)、丙二醛(MDA)的含量。结果丹栀逍遥散能明显抑制由D-GaIN所致的血清中ALT、AST以及肝匀浆中MDA含量的升高,并提高肝匀浆中SOD和GSH-PX的含量,其中大部分差异有统计学意义(P<0.05)。结论丹栀逍遥散对D-GaIN所致的大鼠急性肝损伤具有保护作用。     

丹芪活肝胶囊对D-氨基半乳糖致大鼠肝损伤的保护作用

目的观察丹芪活肝胶囊对D-氨基半乳糖（D—GalN）所致大鼠肝损伤的保护作用。方法sD大鼠60只,随机分6组,分别为正常对照组,模型组,联苯双酯组,丹芪活肝胶囊高剂量组、中剂量组、低剂量组。大鼠分别灌胃给药,1次／d,连续7d,末次给药24h后,除正常对照组外,其余各组大鼠均腹腔注射D．GalN600mg,／kg,24h后采血,检测血清ALT、AST活性及TP、ALB、TBIL含量并观察肝组织病理学变化。结果丹芪活肝胶囊能降低D—GalN所致大鼠肝损伤血清ALT、AST活性,提高TP、ALB含量,降低TBIL含量。其中大部分差异有显著性（P〈0．05）。丹芪活肝胶囊高、中剂量组能明显改善肝组织损伤的程度。结论丹芪活肝胶囊对D—GalN诱导的大鼠肝损伤有保护作用,其作用机制可能与保护肝细胞膜、改善蛋白代谢有关,具体机制还需进一步实验研究。     

茵陈蒿汤提取物保肝作用的血清药理学研究

茵陈蒿汤出自《伤寒论》,由茵陈蒿、栀子、大黄组成,能清热利湿,解毒退黄,主要用于治疗湿热黄疸症。现代研究表明,它具有利胆、保胆、解热抗炎镇痛、抗菌抗病毒的作用。利胆保肝作用是本方临床治疗多种黄疸及传染性肝炎、急性肝炎、急性病毒性肝炎、重型肝炎的主要药理基础。本实验室既往研究已观察到茵陈蒿汤醇提物的保肝作用优于其水提物。本文通过CCl4、D-GalN LPS和BCG LPS诱导小鼠肝损伤观察茵陈蒿汤醇提物的氯仿、乙酸乙酯萃取部位及剩余乙醇提取物降低ALT活性的作用,试图分析茵陈蒿汤保肝作用的效应物质基础。1实验材料1.1动…