ELISA检测襄虫病人血清循环抗原及其在疗效考核方面的应用

用快速双抗体夹心ＥＬＩＳＡ检测囊虫病人血清中的循环抗原，其阳性率为６７．５％；正常人血有的假阳性率为５．３３％；包虫、肝吸虫和弓形虫病人血清的交叉反应率分别为６．９％、７．５％和１２．５％。结果表明．该法与常规双抗体夹心ＥＬＩＳＡ相比具有较为敏感、快速、稳定和节省血清、试剂用量的优点，但其特异性有待进一步提高。用快速双抗体夹心ＥＬＩＳＡ和间接型ＥＬＩＳＡ检测第１至１０疗程囊虫病人血清中的循环抗原和抗体，表明检测循环抗原考核囊虫病人的治疗效果优于检测抗体     

REGULATING FUNCTION OF COENZYMIZATION AND DECOENZYMIZATION OF THE LACTATE DEHYDROGENASE ISOZYMES IN THE MOUSE TISSUES DURING HYPOXIA

Objective. To study the characteristics of changes of LDH enzyme patterns of mice under slight hypoxia.Methods. Mice treated with artificial hypoxia, various tissues were made for the test of LDH enzymatic activity by the specific staining technique. LDH (1 -5) relative percentage enzymatic activity (RPEA) were measured with CS-910 dual-wavelength thin layer chromatography scanner.Results. The RPEA of LDH isozymes of various tissues after slight hypoxia shifted to the isozymes LDH1 and LDH2, whose principal subunits are H subunits, and the RPEA of LDH,(H4), LDH2(H3M) increased, while RPEA of LDH5(M4) in various tissues decreased prominently except the cardiac muscle, and that of LDH4(HM3) decreased as well. After polyacrylamide gel electrophoresis (PAGE) of the hypoxia treated cardiac muscle specimen was made, activity subbands originated regularly in the isozyme patterns of LDH, with the regularity of LDH1 (0 subband), LDH2 (0-1 subbands), LDH3 (0-2 subbands), LDH4 (1-3 subbands), LDH5 (2-4 subbands).     

兔乳酸脱氢酶C4的分离纯化及动力学研究

建立兔乳酸脱氢酶Ｃ４（ＬＤＨ－Ｃ４）分离纯化的新方法，研究该酶的动力学特性。采用Ｂｌｕｅ Ｄｅｘｔｅｒａｎ和Ｓｅｐｈａｒｏｓｅ ４Ｂ交联制成Ｂｌｕｅ Ｄｅｘｔｒａｎ－Ｓｅｐｈａｑｒｏｓｅ ４Ｂ染料配体亲和层析柱，结合ＱＡＥ－Ｓｅｐｈａｄｅｘ Ａ４５０离子交换层析分离兔ＬＤＨ－Ｃ４，以作图法测定该酶动力学数据。     

猪囊尾蚴抗原的免疫化学研究

应用琼脂双扩散(DID)、免疫电泳(IEP)、聚丙烯酰胺凝胶电泳(PAGE)和十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)对猪囊尾蚴的各部分抗原,即完整幼虫、囊液、头节和囊壁抗原进行分析。实验结果表明,4种抗原间存在相同的抗原成分。囊液中含有糖蛋白和脂蛋白。囊液、头节和囊壁蛋白质的主要分子量均在100KDa以下。囊液与头节有3条分子量相同的主带(60、46、38KDa),与囊壁只有1条分子量(46KDa)相同主带。从免疫学和生物化学角度初步探索了囊液、头节和囊壁蛋白质之间的相互关系及主要蛋白质组分。     

Contribution of phosphodiesterase isozymes to the regulation of the L-type calcium current in human cardiac myocytes

1. To determine the contribution of the various phosphodiesterase (PDE) isozymes to the regulation of the L-type calcium current (I_Ca(L)) in the human myocardium, we investigated the effect of selective and non-selective PDE inhibitors on I_Ca(L) in single human atrial cells by use of the whole-cell patch-clamp method. We repeated some experiments in rabbit atrial myocytes, to make a species comparison.
2. In human atrial cells, 100 μM pimobendan increased I_Ca(L) (evoked by depolarization to +10 mV from a holding potential of −40 mV) by 250.4±45.0% (n=15), with the concentration for half-maximal stimulation (EC₅₀) being 1.13 μM. I_Ca(L) was increased by 100 μM UD-CG 212 by 174.5±30.2% (n=10) with an EC₅₀ value of 1.78 μM in human atrial cells. These two agents inhibit PDE III selectively.
3. A selective PDE IV inhibitor, rolipram (1–100 μM), did not itself affect I_Ca(L) in human atrial cells. However, 100 μM rolipram significantly enhanced the effect of 100 μM UD-CG 212 on I_Ca(L) (increase with UD-CG 212 alone, 167.9±33.9, n=5; increase with the two agents together, 270.0±52.2%; n=5, P<0.05). Rolipram also enhanced isoprenaline (5 nM)-stimulated I_Ca(L) by 52.9±9.3% (n=5) in human atrial cells.
4. In rabbit atrial cells, I_Ca(L) at +10 mV was increased by 22.1±9.0% by UD-CG 212 (n=10) and by 67.4±12.0% (n=10) by pimobendan (each at 100 μM). These values were significantly lower than those obtained in human atrial cells (P<0.0001). Rolipram (1–100 μM) did not itself affect I_Ca(L) in rabbit atrial cells. However, I_Ca(L) was increased by 215.7±65.2% (n=10) by the combination of 100 μM UD-CG 212 and 100 μM rolipram. This value was almost 10 times larger than that obtained for the effect of 100 μM UD-CG 212 alone.
5. These results imply a species difference: in the human atrium, the PDE III isoform seems dominant, whereas PDE IV may be more important in the rabbit atrium for regulating I_Ca(L). However, PDE IV might contribute significantly to the regulation of intracellular cyclic AMP in human myocardium when PDE III is already inhibited or when the myocardium is under β-adrenoceptor-mediated stimulation.

     

槟榔与白胡椒对猪囊尾蚴形态学改变的影响

目的　观察研究中药槟榔、白胡椒对体外培养的猪囊尾蚴形态学改变的影响。方法　取经槟榔、白胡椒药液作用不同时间的囊尾蚴进行大体形态及扫描电镜观察。结果　经槟榔、白胡椒分别作用 3 0min ,1h后所有虫体蠕动均停止。槟榔、白胡椒对囊尾蚴分别作用 2 0min ,40min ,虫体表面开始出现部分剥蚀区 ,且随时间延长 ,其剥蚀区增大。结论　两种药物作用于囊尾蚴的效果均好 ,可以作为人或猪囊虫病的治疗药物     

Evagination of Taenia solium cysticerci: a histologic and electron microscopy study

BACKGROUND: After a human being ingests a cysticercus, the larval stage of Taenia solium, the cysticercus gradually develops toward the adult parasite. In this paper, we describe the sequential progress of evagination of cysticerci. METHODS: Intact cysticerci were obtained from swine muscle, and incubated in bovine bile to stimulate evagination. Dissecting, light, and electron microscopy of whole parasites and histologic sections were used for photographic registers. RESULTS: The first event was the widening of the opening of the bladder wall for the scolex and neck to emerge. The two chambers that conform the cysticercus were identified. Histologic sections provided explanation for the conformation of the spiral canal.The scolex uncoils during evagination but does not turn inside out. CONCLUSIONS: The scolex and the neck comprise a different structure from the bladder wall, although they are contiguous.     

广西中枢神经系统疾病患者囊虫感染调查及流行病学分析

目的了解广西壮族自治区中枢神经系统（CNS）疾病患者中脑囊虫感染状况，分析其流行特征。方法用ELISA法检测2000-2008年来自治区疾控中心就诊的CNS疾病患者的囊虫特异性抗体，并对抗体阳性患者进行囊虫病流行病学分析。结果2736例CNS疾病患者血清和／或脑脊液中囊虫特异性抗体阳性者403人，阳性率为14．73％。阳性者分布于全自治区55个县（市、区），大多集中在广西南部的南宁市及周边的贵港、武呜、宾阳、横县等市县，在阳性者的构成比中，农村高于城市，男性多于女性，成人多于青少年，农民多于其他职业者；2000年以后检出的囊虫抗体阳性率整体呈逐渐下降的趋势．但流行的区域却逐渐扩大，尤其在交通、经济落后，环境卫生较差的边远山区。结论脑囊虫感染是引起广西人群CNS疾病的一个重要原因之一，在重点地区特定人群中开展囊虫病防治，是减少CNS疾病发生的重要手段。因此．今后在全自治区，尤其在边远山区仍需加强囊虫病防治工作。     

猪囊尾蚴特异循环免疫复合物致病作用的研究

本文对脑型和混合型猪囊尾蚴病患者脑脊液（ＣＳＦ）中特异循环免疫复合物（ＣＩＣ）进行了检测，其结果与患者ＣＴ表现及吡喹酮治疗第一个疗程过程中患者出现的高颅压情况存在一定联系。人工免疫复合物（ＩＣ）对ＢＡＬＢ／ｃ鼠作用后进行病理检查，发现脑实质和肾脏小血管均出现充血现象．部分小血管内皮细胞遭到破坏。因此认为特异循环免疫复合物可能是脑型、混合型猪囊尾蚴病患者在第一疗程中出现高颅压反应的主要因素之一。