复方栀子冲剂栀子甙薄层扫描测定

应用薄层扫描法测定了复方栀子冲剂中栀子甙的含量，对实验条件，以正交试验法进行优选，确定了最佳方案，排除了干扰，得到了满意的效果。     

辣蓼颗粒成型工艺的研究

目的探讨辣蓼颗粒的成型工艺条件。方法考察不同辅料即乳糖、玉米淀粉和糊精对浸膏粉吸湿性的影响；用正交试验法优选制粒工艺条件及评价颗粒的流动性与吸湿性等。结果3种辅料均可明显降低浸膏粉的吸湿性；最佳制粒工艺条件为浸膏粉与玉米淀粉、乳糖按2：2：1的比例混匀，以75％乙醇为润湿剂制软材，湿粒以70％温度干燥，成品颗粒的流动性好；临界相对湿度为75％。结论混合辅料（玉米淀粉和乳糖）可改善浸膏粉的成型性和颗粒剂的抗湿性；辣蓼颗粒制粒工艺条件的优化为产品的工业生产环境控制提供了科学依据。     

补肾抗衰口服液对大鼠衰老模型免疫器官影响的实验研究

研究补肾抗衰口服液对大鼠衰老模型免疫器官胸腺和脾脏的影响.结果显示,模型组大鼠胸腺和脾脏重量减轻,胸腺重/体重比值、脾重/体重比值减小,胸腺组织学观察,显示萎缩改变;药物组大鼠胸腺和脾脏重量、胸腺重/体重、脾重/体重比值接近正常对照组,胸腺组织学观察,未显示萎缩改变.本研究结果表明,补肾抗衰口服液能延缓胸腺和脾脏萎缩,保护机体的正常免疫功能,提示该药有抗衰老的作用.     

薄层扫描法测定复脉定冲剂中远志有效成分的含量

采用薄层扫描法对复脉定冲剂中远志有效成分３，４，５－三甲氧基反式肉桂酸乙酯的含量进行测定。方法简单、快速，结果可靠，可作为该制剂的质控标准。     

健脾降脂颗粒中绿原酸的含量测定

目的　研究健脾降脂颗粒中绿原酸的含量测定方法。方法　采用高效液相法。色谱柱YWGC18(2 5 0mm× 4 6mm ,10 μm) ,室温 ,10 %乙腈─冰醋酸 (5∶0 1)为流动相 ,流速为 1mL/min ,检测波长 32 9nm。结果　绿原酸在 0 0 2 0 8～ 0 10 4 μg范围内呈线性关系 (r =0 9994 ,n =5 ) ,平均加样回收率为 96 2 3% ,RSD为 0 798%。结论　本方法准确、可行 ,可用于健脾降脂颗粒的质量控制     

复方广金钱草冲剂检验方法研究

应用薄层层析法，对制剂中广金钱草、黄芩、陈皮所含黄酮类成分，在同一色谱中得到的分离与鉴定，并以薄层一分光光度法对制剂中大黄所含成分大黄素进行了含量测定，方法重现性好，回收率为１００．１％，ＲＳＤ为１．０９％。     

基于骨免疫微环境探讨补肾活血方防治绝经后骨质疏松症的研究进展

骨质疏松症（OP）是我国人群中最常见的多发病,好发于绝经后的老年妇女,增加了患者骨折的风险。绝经后骨质疏松症（PMOP）是一种以骨量减少和骨折风险增加为特征的全身性疾病,主要是绝经后雌激素水平显著降低导致的。除了雌激素缺乏对骨骼的直接负面影响外,绝经后妇女免疫状态的改变间接导致骨骼的持续破坏,因为绝经后妇女通常表现出慢性低级别炎症表型,细胞因子表达和免疫细胞谱发生改变。PMOP已严重危害我国女性健康。补肾活血方是一种应用广泛的补肾活血中药,近年来,随着我国临床医师与患者越来越重视中医药,补肾活血方被应用于多种疾患,包括骨科的多种疾病,其对PMOP的防治效果亦明显。该文基于骨免疫微环境,以骨代谢对PMOP产生影响为基础,论述补肾活血方的作用机制。     

Light bodies in human pituitary adenomas

Summary Light bodies are large cytoplasmic granules originally described in the gonadotrophic cells of the rat pituitary gland. In order to determine whether similar bodies occur in the human anterior pituitary gland, 89 pituitary adenomas and periadenomatous tissue from 20 cases were examined by transmission electron microscopy. Double membrane bound bodies with filamentous internal structure identical to rodent light bodies were identified in 10 hormone-producing adenomas: 5 PRL, 1 PRL-GH, 2 GH, and 2 ACTH-producing tumours. No light bodies were found in the remaining 79 tumours nor in the pituitary cells in periadenomatous tissue from 20 cases. These results show that some human pituitary adenomas may contain light bodies identical to those seen in gonadotrophs of rat pituitary.Abbreviations PRL prolactin - GH growth hormone - ACTH adenocorticotropic hormone - FSH follicle-stimulating hormone - LH luteinizing hormone     

补肾化瘀解毒方药物血清对肺癌A549/DDP细胞的耐药逆转及MRP表达的影响

目的：探讨补肾化瘀解毒方药物血清对肺癌细胞耐药逆转作用及机制。方法:采用MTT及流式细胞术，分别观察补肾化瘀解毒方药物血清对肺癌A549/DDP耐药细胞的细胞毒作用和多药耐药相关蛋白（MRP）表达的影响。结果:补肾化瘀解毒方药物血清对肺癌耐药细胞有一定的杀伤作用，能增强顺铂（DDP）对肺癌敏感细胞和耐药细胞的杀伤作用，显著降低MRP的表达（P＜0.01），但对维拉帕米（VRP）的协同逆转作用不明显。结论:补肾化瘀解毒方药物血清具有增效和耐药逆转作用，其机制可能与其抑制肺癌耐药细胞MRP的表达有关。     

Control of fluid shear response in circulating leukocytes by integrins

Recent evidence shows that circulating leukocytes respond not only to humoral inflammatory mediators but also to fluid stresses. Application of fluid shear stress (of the order of 1–10 cm² to fresh migrating leukocytes leads to initial retraction of pseudopods, an important step to facilitate normal passage of leukocytes through the microcirculation and to prevent spreading on the endothelium. The ability to respond to fluid shear stress, however, may be regulated under different physiological conditions. In the current study, we examine the role of integrins in the fluid shear response as measured by pseudopod retraction with the use of antibodies against human neutrophil ₁ and ₂ integrins. Neutrophils adhering via ₂ integrins exhibit normal ability to project pseudopods and to migrate. Such cells show normal response to fluid shear with rapid pseudopod retraction. In contrast, attachment via ₁ integrins leads to firmly adhesive leukocytes, spreading and almost no cell migration. Such leukocytes exhibit a significantly attenuated ability for pseudopod retraction under fluid shear. These results suggest that integrins may serve as a regulating mechanism for fluid shear response in human leukocytes. Attachment via ₁ integrins may lead to an abolishment of the fluid shear response. © 2002 Biomedical Engineering Society. PAC2002: 8716-b, 8719Tt