Immunoelectronmicroscopical demonstration of in vivo bound complement C 3 in psoriatic lesions

Summary The presence of in vivo bound complement (C3) within the stratum corneum of 4 psoriatic cases is demonstrated ultrastructurally by the use of the peroxidase—antiperoxidase multistep technique. The positive reaction product was located in the intercellular space and on the surface of the parakeratotic cells within the horny layer. The membranes of the horny cells were not altered and peroxidase granules could also not be detected within their cytoplasm. The explanation of this finding as well as its pathogenetic significance is discussed.Supported by the Deutsche Forschungsgemeinschaft. The technical assistance of Mrs. I. Pfab and Mrs. A. Hess is gratefully acknowledged     

2型糖尿病甲状腺功能及甲状腺自身抗体的评价

目的分析2型糖尿病患者甲状腺功能变化和自身抗体水平。方法化学发光法对78例2型糖尿病患者以及74例正常对照组血清FT3、FT4、TSH、aTPO、aTG进行检测分析。结果2型糖尿病甲状腺功能异常者占19．23％。其中15．38％表现为甲状腺功能减退,发生率显著高于正常对照组（P〈0．05）;3．85％的患者表现为甲状腺功能亢进,与正常对照组比较无明显差异（P〉0．05）。2型糖尿病患者aTPO阳性率为52．56％,与正常对照组比较有极显著差异（P〈0．01）;aTG阳性率16．67％,与正常对照组比较统计学差异不明显（P〉0．05）。结论2型糖尿病患者甲状腺功能异常发生率较高,主要表现为甲状腺功能减低和甲状腺自身抗体aTPO的增高,对2型糖尿病患者定期随访甲状腺功能很有必要。     

早孕妇女活动性巨细胞病毒感染的调查及其宫内感染检测方法的研究

本文对北京、青岛及沈阳三市的1202名早孕妇女进行了原发性HCMV感染的临床调查,血清中特异性HCMV—IgM和IgA抗体的酶联免疫吸附法检测;分析了患病的风险因素;并采用辣根过氧化物酶复合物(PAP)免疫组化技术检测其胚胎绒毛中的HCMV,以便建立一种宫内感染诊断的方法。利用透射电镜和聚合酶链反应(PCR)技术来验证此方法的可靠性,结果显示PAP法的阳性及阴性率与电镜观察的符合率为100％,与PCR法的符合率分别为97.0％与86.6％,实为一种敏感、经济、易行的宫内诊断方法。