骨痹通天丸抗炎镇痛免疫作用研究

实验动物口服骨痹通天丸5．58g／kg．2．79g／kg，观察本品对炎症反应，局部疼痛和免疫功能的影响。结果表明，骨痹通天丸的两个剂量组对大鼠蛋清性关节肿具有不同程度的抑制作用（P＜0．01或0．05）；对二甲苯性耳廓肿仅大剂量组具有明显抑制作用（P＜0．01）；对小鼠热刺激所致的疼痛反应具有不同程度的抑制作用（P＜0．01或0．05）；对化学刺性疼痛两个剂量组均有明显抑制作用（P＜0．01）；并可抑制小鼠重要免疫器官的增长和巨噬细胞的吞噬功能（P＜0．05），对血清溶血素未显示其效应。     

人胎盘组织液抗炎药效学研究

目的研究人胎盘组织液的主要药效作用。方法建立二甲苯所致小鼠耳肿胀及大鼠棉球肉芽肿的药理模型，观察人胎盘组织液对炎症模型动物的抗炎作用。结果与模型对照组相比，连续7d肌肉注射0．1mL/kg剂量的人胎盘组织液对二甲苯所致小鼠耳肿胀起到明显抑制作用，且差异显著（P〈0．01）；连续6d肌肉注射0．1mL／kg剂量的人胎盘组织液对大鼠棉球肉芽组织增生起到明显抑制作用，且差异显著（P〈0．01）。结论人胎盘组织液具有明显的抗炎作用。     

Rapid non-genomic inhibitory effects of glucocorticoids on human neutrophil degranulation

Background: Glucocorticoids acting as anti-inflammatory or immunosuppressive drugs have been shown to exert most of their effects genomically. Recent findings suggest that non-genomic activity might be relatively more important in mediating the therapeutic effects of high-dose pulsed glucocorticoid. However, few non-genomic anti-inflammatory effects were reported, much less non-genomic mechanisms.Objective: This study was performed to investigate the nongenomic effects of glucocorticoids on human neutrophil degranulation.Methods: Purified human neutrophils were pretreated with 6 -methylprednisolone or hydrocortisone for 5 min, and then primed with N-formyl-methionyl-leucyl-phenylalanine (fMLP) (10^–6 M) or phorbol myristate acetate (PMA) (50 ng/ml) in the presence of cytochalasin B. The release of two markers of neutrophil granules, lactoferrin and myeloperoxidase, was measured by ELISA and enzymology methods respectively.Results: Both 6 -methylprednisolone (10^–5–10^–4 M) and hydrocortisone (10^–4 M) showed significant inhibitory effects on neutrophil degranulation within 5 min after fMLP administration. For PMA stimulated degranulation, 6 -methylprednisolone (10^–4 M) showed significant inhibitory effects (p < 0.01), while hydrocortisone (10^–4 M) only showed an inhibitory tendency (P > 0.05). Neither RU486 (10^–5 M) nor cycloheximide (10^–4 M) could alter the inhibitory effects of glucocorticoids.Conclusion: Our results demonstrate that megadoses of glucocorticoids exert rapid inhibitory effects on human neutrophil degranulation at the cellular level via a new mechanism that is independent of corticosteroid type II receptor occupation or protein synthesis. We infer that these effects may be very important when glucocorticoids act as anti-inflammatory drugs during pulse therapy.Received 20 May 2004; returned for revision 21 July 2004; accepted by M.J. Parnham 23 September 2004L. Liu and Y. X. Wang contributed equally to this work.     

活血止痛方的抗炎及抗血栓形成作用

目的 :观察活血止痛方对小鼠的抗炎作用及对大鼠血栓形成的影响。方法 :用二甲苯作为致炎剂 ,观察活血止痛方对二甲苯所致小鼠耳廓肿胀的影响 ,采用大鼠体外颈总动脉—颈外静脉血流旁路形成血小板血栓法 ,观察活血止痛方的抗血栓形成作用。结果 :活血止痛方能显著抑制耳肿胀 (P<0 .0 1) ,能显著减轻血栓湿重 (P<0 .0 1)。结论 :活血止痛方有明显的抗炎、抗脑血栓形成作用     

虎杖提取物抗炎作用的实验研究

[目的]考察虎杖的乙酸乙酯提取物的抗炎作用及初步机制。[方法]分别以地塞米松、阿司匹林和环磷酰胺为阳性对照药,采用虎杖的乙酸乙酯提取物对小鼠和大鼠的多种炎症模型进行抑制实验。[结果]虎杖的乙酸乙酯提取物对角叉菜胶所致大鼠足跖肿胀有显著抑制作用(P<0.00 J);对小鼠、大鼠纸片法所致肉芽肿有显著抑制作用(P<0.01);对腹腔注射醋酸所致小鼠毛细血管通透性增高有显著抑制作用(P<0.01);对角叉菜胶所致小鼠肿胀足中炎症介质PGE2合成有显著抑制作用(P<0.001);对三硝基氯苯诱导的小鼠迟发性超敏反应有显著抑制作用(P<0.01);对肾上腺切除大鼠纸片法致肉芽肿(P<0.01)及角叉菜胶所致足跖肿胀(P<0.05)有显著抑制作用,但其抑制率均较正常大鼠相应实验抑制率有所降低。[结论]虎杖的乙酸乙酯提取物有一定的抗炎作用,其作用机制可能是抑制炎症介质PGE2的合成、抑制细胞免疫及与垂体-肾上腺皮质系统有关。     

银屑宁胶囊抗炎清热作用的药理研究

目的：探讨银屑宁胶囊的抗炎清热作用。方法：银屑宁胶囊灌胃大小鼠一段时间后，用二甲苯诱发小鼠耳廓部急性炎性水肿和小鼠腹部皮肤毛细血管通透性亢进，将新鲜蛋清和鹿角菜胶悬液大鼠皮下注射造成其足跖肿胀，同时用2，4-硝基苯酚溶液和干酵母悬液诱发大鼠全身发热。结果：银屑宁胶囊对大鼠鹿角菜胶，蛋清性足肿胀及小鼠二甲苯所致的耳肿胀有明显的抑制作用，可明显拮抗小鼠皮肤毛细血管通透性亢进。同时能明显抑制干酵母及2，4-二硝基苯酚所引起的大鼠全身性发热。结论：银屑宁胶囊具有良好的抗炎清热作用。     

盆腔舒胶囊抗炎镇痛作用及对子宫收缩影响

目的： 通过盆腔舒胶囊对慢性炎症的抗炎及镇痛作用研究，探讨其治疗慢性盆腔炎的作用机制。方法：抗炎作用采用二甲苯小鼠耳廓肿胀模型、大鼠子宫炎症模型、小鼠滤纸片肉芽肿增生模型；镇痛作用采用小鼠热板法及小鼠扭体反应法；对家兔子宫的影响采用对正常家兔体外子宫平滑肌收缩的影响和对缩宫素所致家兔体外子宫平滑肌收缩的影响两种方法。结果：盆腔舒胶囊抗炎作用优于妇科千金片，接近氢化可的松；镇痛作用强于妇科千金片，接近阿司匹林；对正常家兔和对缩宫素所致家兔体外子宫平滑肌的抑制作用优于妇科千金片，接近阿托品。结论：盆腔舒胶囊具有良好的抗炎，镇痛及抑制子宫平滑肌收缩的作用。     

镇关灵治疗类风湿性关节炎的药效学研究

目的 探索镇关灵治疗类风湿性关节炎(rheumatoida~dtis，RA)的作用。方法 以佐剂性关节炎(AA)、蛋清性关节炎、角叉菜胶性关节炎为实验病理目标，建立二硝基氟苯所致小鼠迟发型超敏反应(DTH)、棉球肉芽肿及醋酸所致小鼠腹腔毛细血管通透性增高和扭体反应等病理模型。结果 镇关灵能显著抑制大鼠佐剂性关节炎、蛋清性关节炎、角又菜胶所致炎症的足跖肿胀度和棉球肉芽肿，抑制由醋酸引起的小鼠扭体反应和腹腔毛细血管通透性增高，并可抑制DTH。结论 镇关灵具有良好的抗炎镇痛免疫抑制作用，对类风湿性关节炎具有明显的预防和治疗作用。     

Hepatoprotective effect of mulberry water extracts on ethanol-induced liver injury via anti-inflammation and inhibition of lipogenesis in C57BL/6J mice

Many plant extracts and their bioactive substances are well recognized for their potential to exert as chemoprotective agents against common alcoholic liver injury. In this study, the effects of Mulberry water extracts (MWE) treatment in the prevention of alcohol-induced liver injury were investigated in mice. MWE contain many nutrients and bioactive substances, including fifteen types of polyphenols and anthocyanin compounds. The parameters of histopathology, immunohistochemistry, antioxidant defense and proinflammatory mediator demonstrated the inhibitory effect of MWE on alcohol-induced liver injury. Plasma and hepatic content analysis showed that MWE inhibited the levels of liver injury biomarkers (AST, ALT and ALP), triglyceride (TG) and cholesterol (TC). Furthermore, treatment with MWE lessened the expression of lipid synthesis-related proteins, increased the p-AMPK/AMPK ratio and PPAR-α. Fatty acid oxidation and export via microsomal triglyceride transfer protein (MTP) were both activated as well as carnitine palmitoyltransferase-1 (CPT1). These results suggested that MWE prevents alcohol-induced liver injury through the activation of the AMPK and PPAR-α signal. This may be mediated by multiple pathways, including reduced lipid accumulation and lipid synthesis, increased fatty acid transport and fatty acid oxidation responses, decreased oxidative stress and facilitated anti-inflammation.