全文获取类型
收费全文 | 5019篇 |
免费 | 220篇 |
国内免费 | 157篇 |
专业分类
耳鼻咽喉 | 36篇 |
儿科学 | 144篇 |
妇产科学 | 49篇 |
基础医学 | 655篇 |
口腔科学 | 170篇 |
临床医学 | 298篇 |
内科学 | 791篇 |
皮肤病学 | 36篇 |
神经病学 | 328篇 |
特种医学 | 83篇 |
外国民族医学 | 1篇 |
外科学 | 345篇 |
综合类 | 633篇 |
预防医学 | 440篇 |
眼科学 | 19篇 |
药学 | 955篇 |
中国医学 | 323篇 |
肿瘤学 | 90篇 |
出版年
2023年 | 56篇 |
2022年 | 89篇 |
2021年 | 126篇 |
2020年 | 98篇 |
2019年 | 112篇 |
2018年 | 81篇 |
2017年 | 106篇 |
2016年 | 136篇 |
2015年 | 132篇 |
2014年 | 252篇 |
2013年 | 391篇 |
2012年 | 228篇 |
2011年 | 287篇 |
2010年 | 223篇 |
2009年 | 194篇 |
2008年 | 216篇 |
2007年 | 245篇 |
2006年 | 211篇 |
2005年 | 180篇 |
2004年 | 167篇 |
2003年 | 135篇 |
2002年 | 138篇 |
2001年 | 104篇 |
2000年 | 99篇 |
1999年 | 110篇 |
1998年 | 76篇 |
1997年 | 74篇 |
1996年 | 60篇 |
1995年 | 75篇 |
1994年 | 68篇 |
1993年 | 63篇 |
1992年 | 47篇 |
1991年 | 43篇 |
1990年 | 41篇 |
1989年 | 56篇 |
1988年 | 45篇 |
1987年 | 40篇 |
1986年 | 32篇 |
1985年 | 56篇 |
1984年 | 45篇 |
1983年 | 31篇 |
1982年 | 36篇 |
1981年 | 35篇 |
1980年 | 29篇 |
1979年 | 39篇 |
1976年 | 31篇 |
1974年 | 27篇 |
1973年 | 42篇 |
1972年 | 30篇 |
1971年 | 27篇 |
排序方式: 共有5396条查询结果,搜索用时 15 毫秒
1.
2.
The peri-implantation period of pregnancy is critical for conceptus development, implantation, and signaling for establishment of pregnancy. This study evaluated the effects of bisphenol A (BPA) on proliferation, adhesion, and migration of porcine trophectoderm (pTr2) cells, expression of transporters of arginine and synthesis of amino acids. All concentrations of BPA decreased proliferation and adhesion of pTr2 cells after 96 h compared to the control group. Lower concentrations of BPA (1 × 10−9, 1 × 10-8, 10-7M) increased (P < 0.05), but higher concentrations of BPA (1 × 10-5, 1 × 10-4 M) decreased migration of pTr2 cells. BPA increased expression of SLC7A1 mRNA at lower concentrations (1 × 10−9 to 1 × 10-6M) and SL7A6, another cationic acid transporter, at higher concentrations (1 × 10-5, 1 × 10-4 M). BPA also down-regulated the expression of IGF1 and IGF1 receptor at concentrations of 1 × 10-7 to 1 × 10-4 M compared to the control group. The expression of mRNAs for aquaporins (AQP) 3 and 4 were reduced at all concentrations of BPA, but at lower concentrations of BPA, (1 × 10−9 to 1 × 10-8M) expression of AQP9 mRNA increased and the expression of AQP11 was not affected by BPA (P > 0.05). There was an inhibitory effect of BPA on the release of synthesis of asparagine, threonine, taurine, tryptophan, and ornithine into the culture medium by pTr2 cells. Collectively, BPA adversely affected the expression of transporters for cationic amino acids like arginine, as well as AQPs, IGF1, and IGF1R associated with proliferation, migration, and adhesion of pTr2 cells. Those adverse effects would likely increase pregnancy losses during the peri-implantation period of pregnancy. 相似文献
3.
4.
It is known that low glucose concentrations increase the aspartate and decrease the glutamate content of brain tissue both in vivo and in vitro. To see whether these changes occur in the transmitter compartment or not, the release of aspartate and glutamate evoked by electrical-field stimulation or by high K+ was followed in slices of rat hippocampus superfused with 5 or 0.2 mM glucose. Superfusion with 0.2 mM glucose increased the evoked release of aspartate about ten times and that of glutamate about threefold. This shift in the ratio of aspartate to glutamate released was accompanied by a similar increase in the relative amount of aspartate contained in the slices. The high evoked release of aspartate and glutamate was well maintained, provided 0.5 mM glutamine was added to the medium. Changing the concentration of glucose after the first period of stimulation rapidly altered the relative amounts of aspartate and glutamate released but not the enhanced release of glutamate. The large evoked release of both aspartate and glutamate in 0.2 mM glucose was almost entirely Ca2+-dependent. The relative amounts of aspartate and glutamate released by 50 mM K+ also changed when the glucose concentration was reduced. Results suggest two effects of low glucose concentrations: an increase in the overflow of synaptically released glutamate due to a decreased uptake and an increase in the proportion of aspartate to glutamate formed and released from the transmitter pool. These observations are consistent with the interpretation that these two transmitters can be released in different proportions from the same terminals. 相似文献
5.
OBJECTIVE: The purpose of this study was to characterize the etching behavior of titanium in concentrated sulfuric acid and discuss its application on surface modification of titanium for biological use. METHODS: Commercially pure titanium (cpTi) plate was etched in 48% H2SO4 at RT -90 degrees C for 0.25-8 h. The weight loss was derived from the weight differences before and after etching. The surfaces after etching were characterized by surface roughness, X-ray diffractometry, and scannning electron spectroscopy. The apparent activation energy of the dissolution of cpTi into acid was derived from an Arrhenius plot of the rate of weight loss versus the acid temperature. RESULTS: The surface roughness of cpTi increased with the acid temperature and etching time. The surface roughness was strongly related to the weight loss. The weight loss increased drastically with the acid temperature after an initial period, which shortened with increasing acid temperature. The apparent activation energy for the dissolution of cpTi in H2SO4 was derived as 67.8 kJ/mol. SIGNIFICANCE: This study indicates that etching with concentrated sulfuric acid is an effective way to modify the surface of titanium for biological applications. 相似文献
6.
目的:观察高渗氯化钠高氧液对失血性休克家兔动脉血气和血乳酸值的影响,评价其对失血性休克的早期救治效果。方法:制备高渗氯化钠溶液(HS)、生理盐水高氧液(NSO)和高渗氯化钠高氧液(HSO)。30只雄性家兔制备失血性休克模型[于10min内使平均动脉压(MAP)降至40mmHg(1mmHg=0.133kPa),维持60min],随机分为NSO,HS,HSO组3个治疗组。分别按6mL/kg剂量5min内静脉输入NSO,HS和HSO。记录休克前后及给药后心率(HR)、呼吸(RR)、MAP及尿滴(UD),测定休克前、休克60min,给药后30,60,120min时血乳酸(BL)和动脉血气值。最后观察尸肺,测定肺系数。结果:HS和HSO组均显著地改善MAP,HR和UD,降低BL,改善代谢性酸中毒,肺系数明显低于NSO组。HSO与NSO及HS比较,能更显著地降低血BL,提高动脉血氧饱和度(SaO2)和动脉血氧分压(PaO2)。结论:HSO较HS和NSO能更显著地降低血BL,提高SaO2和PaO2,对失血性休克的早期救治具有较高的使用价值。 相似文献
7.
Cultured human proximal tubule cell monolayers maintained on permeable supports were treated simultaneously with the aminoglycoside antibiotic, gentamicin, and poly-
-aspartic acid (PAA), an inhibitor of aminoglycoside nephrotoxicity. Following 4 days of exposure, cell monolayers were placed into Ussing chambers to allow monitoring of transepithelial electrical properties. For each of the three cell isolatation examined, aminoglycoside-induced alterations in electrogenic transport, reflected by changes in short-circuit current (Isc), as well as alterations in paracellular properties, indicated by changes in transepithelial electrical resistance (RT), were diminished in the presence of PAA. Alterations resulting from selective basolateral exposure to gentamicin were unchanged in the case of apically applied PAA and attenuated only when PAA acid was added basolaterally. This is the first demonstration of PAA inhibition of aminoglycoside-induced cellular alterations involving human cells. 相似文献
8.
经临床鉴定静脉内注射小儿氨基酸对治疗胎儿宫内发育迟缓具有肯定效果。在B超监视下,使用小儿氨基酸的孕妇组于用药期间,胎儿生长参数BPD和HC显著高于使用成人氨基酸的孕妇组。10例足月娩出胎儿体重均达到甚至超过2.5kg。由此得出结论:使用小儿氨基酸注射液对于改善胎儿宫内发育迟缓优于成人氨基酸。 相似文献
9.
健脾降脂颗粒中绿原酸的含量测定 总被引:1,自引:0,他引:1
目的 研究健脾降脂颗粒中绿原酸的含量测定方法。方法 采用高效液相法。色谱柱YWGC18(2 5 0mm× 4 6mm ,10 μm) ,室温 ,10 %乙腈─冰醋酸 (5∶0 1)为流动相 ,流速为 1mL/min ,检测波长 32 9nm。结果 绿原酸在 0 0 2 0 8~ 0 10 4 μg范围内呈线性关系 (r =0 9994 ,n =5 ) ,平均加样回收率为 96 2 3% ,RSD为 0 798%。结论 本方法准确、可行 ,可用于健脾降脂颗粒的质量控制 相似文献
10.
E. Angelini M. Teixeira J.-M. Aran E. Ferrary 《European archives of oto-rhino-laryngology》1998,255(7):331-333
Taurine is a β-aminosulfonic acid and is a ubiquitous amino acid whose role in the cochlea is not well established. In this
study, its entry from blood into perilymph was investigated in the guinea pig as animal model. The penetration rate of [3H]taurine (molecular weight 125) into the perilymph of the scala vestibuli was measured 1 and 2 h after the intravenous infusion
of [3H]taurine in nephrectomized animals. Results showed a rate of penetration in perilymph related to plasma at 36 ± 4.7% (n = 5) after 1 h and 43 ± 5.6% (n = 5) after 2 h. Compared to the penetration rate of urea (molecular weight 60) and mannitol (molecular weight 186) reported
previously in rats, a passive entry of taurine into perilymph through the blood-perilymph barrier is suggested.
Received: 30 July 1997 / Accepted: 15 January 1998 相似文献