Agilent 2100 Bioanalyzer在基因差异表达研究中的应用

目的观察Agilent 2100 Bioanalyzer 芯片分析系统(以下简称Bioanalyzer)在基因差异表达研究中的应用。方法应用限制性显示技术分别从正常和热休克处理后的酿酒酵母细胞中分离出cDNA片段,然后再用Bioanalyzer和传统的琼脂糖凝胶电泳技术对RD-PCR产物进行检测分析。结果Bioanalyzer能更快速、敏感地分离和显示差异表达的基因片段,并且通过对差异片段进行定量比较,发现了数个表达有明显差异的基因片段。结论Bioanalyzer在基因差异表达研究中具有重要的应用价值。     

Impacts of the Affordable Care Act's Medicaid Expansion on Women of Reproductive Age: Differences by Parental Status and State Policies

Introduction

We use data from the Behavioral Risk Factor Surveillance System (BRFSS) from 2012 to 2015 to estimate the effects of the Affordable Care Act's (ACA) Medicaid expansions on insurance coverage and access to care for low-income women of reproductive age (19–44).

Primary empty sella syndrome with visual field defects

A patient with an enlarged, asymmetric sella turcica and visual field defects suggestive of a pituitary or parasellar tumor underwent extensive roentgenographic and pituitary function studies. No abnormalities in pituitary luteinizing hormone, follicle-stimulating hormone, thyroid-stimulating hormone, ACTH, prolactin or vasopressin secretion were detected. Growth hormone secretion was provoked by arginine infusion but not by hypoglycemia. Pneumoencephalography revealed air in the sella turcica, and no evidence of tumor. Thus, an enlarged sella turcica in a patient with visual field defects but normal pituitary function may suggest the presence of an “empty sella syndrome.”     

XE-2100全自动血细胞分析仪在慢性肾衰血液透析中的应用研究

目的 探讨利用XE-2100全自动血细胞分析仪检测血液透析患者动脉血红细胞压积来确定血容量的改变。方法 对60例慢性肾衰维持性血液透析的患者,在透析过程中分5次检测透析管路动脉端血液红细胞压积,并根据红细胞压积变化计算血容量(BV)下降比率,同时监测血压变化,以此确定合适的干体重和透析速度。结果 在超滤4小时后有14例患者红细胞压积升高太多,血容量下降超过允许范围(>15％),以至于收缩压下降超过30mmHg,部分患者出现低血压症状。46例患者红细胞压积上升合适,血容量下降正常(<15％),收缩压下降小于30mmHg,无低血压症状出现。结论 在一定的时间内多次检测红细胞压积来计算透析血容量,使血容量下降率控制在15％以内,就可以减少透析性低血压的发生。     

Formation,bioavailability and organoleptic properties of an inclusion complex of femoxetine with β-cyclodextrin

Femoxetine, a new selective serotonin uptake inhibitor with antidepressant properties, possesses a very bitter taste which hinders the development of oral liquid formulations. The present study has shown that it is possible to improve the organoleptic properties by inclusion complexation of the drug with β-cyclodextrin. Phase solubility diagrams and UV-spectroscopic studies revealed the formation of 1 :1 and 1: 2 inclusion complexes, the strongest complex being obtained for femoxetine in free-base form. A microcrystalline inclusion complex was isolated and shown to have the stoichiometric composition of 1: 2 (femoxetine: β-cyclodextrin). In a single dose cross-over study in 5 volunteers, the bioavailability of the solid complex formulated as an aqueous suspension was found to be similar to that observed for a sugar-coated tablet of femoxetine hydrochloride.     

全自动血液分析仪监测肿瘤患者化疗前、后骨髓状态的临床应用

目的 研究肿瘤患者化疗前后白细胞(WBC)、血小板(PLT)、平均血小板体积(MPV)未成熟网织红细胞指数(IRF)、网织红细计数(RET)的变化,了解病人的骨髓状态.方法 用XE-2100全自动血液分析仪进行测定.结果 肿瘤患者化疗期间IRF、RET在化疗5天后即出现明显下降.化疗后15天,IRF、RET、MPV即出现显著上升.结论 全自动血液分析仪能为医生观测病人的骨髓状态提供更敏感的指标.     

Fetal movements registered by the pregnant woman correlated to retrospective estimations of fetal movements from cardiotocographic tracings

After reviewing 30 cardiotocographic tracings from 15 pregnant women who directly registered fetal movements (FM) on the cardiotocographic (CT) tracings, we have found no correlation between the actual number of FM and the FM found by retrospective estimation from CT tracings. Ours is the first study to show this lack of correlation. On the contrary, we have found that by using the “retrospective” or “indirect” method, almost 90% of the FM are overlooked. This is a very important loss of information since registration of FM seems to be a good method for surveillance of the fetus at risk. We also discuss our own methods for overcoming these problems.