Soluble adhesion molecules ICAM-1, VCAM-1, P-selectin in children with Helicobacter pylori infection

AIM: To assess the sICAM-1, sVCAM-1, and sP-selectin levels in children with Helicobacter pylori (H pylori) infection and to evaluate their significance for the morphological changes found in gastric mucosa. METHODS: The study included 106 children: 59 children (55.7%) with chronic gastritis and positive IgG against H pylori, 29 children (27.3%) after previous H pylori infection without the bacterium colonization but with positive IgG against H pylori, and 18 children (17%) with functional disorders of the gastrointestinal system but with normal IgG against H pylori. Endoscopic and histopathological evaluation of gastric mucosa was performed based on the Sydney System classification. The evaluation of sP-selectin, sICAM-1, sVCAM-1 levels in the sera of children was carried out using ELISA test. RESULTS: The assessment of gastritis activity degrees indicated statistically significant values in the antrum and corpus (P＜0.001) of children examined. Serum sVCAM-1 levels were higher in group with gastritis due to H pylori infection than in group without infection and differed statistically (P＜0.05). Serum sVCAM-1 levels proved to be the highest among other adhesive molecules in infected children and decreased after eradication of H pylori. Serum sICAM-1 levels were similar in all examined groups. Serum sP-selectin levels were similar in children with and without H pylori infection. CONCLUSION: Assessment of adhesive molecules (sP-selectin, sICAM-1, sVCAM-1) in the sera of children with active H pylori infection can show the participation of sVCAM-1 in the pathogenesis of gastric mucosal inflammation. sP-selectin and sICAM-1 concentrations in the sera of children with H pylori infection after eradication cannot reveal any significant differences as compared to healthy children.     

安络化纤丸联合硫普罗宁治疗慢性乙型肝炎肝硬化的临床研究

目的 探讨安络化纤丸联合硫普罗宁治疗慢性乙型肝炎肝硬化的临床疗效。方法 选取2021年8月—2023年2月西安市第八医院收治的166例慢性乙型肝炎肝硬化患者,按随机数字表法将所有患者分为对照组和治疗组,每组各83例。对照组静脉滴注注射用硫普罗宁,每次将0.2 g加入0.9%氯化钠注射液250 mL中充分稀释后给药,1次/d。治疗组在对照组治疗基础上口服安络化纤丸,6 g/次,2次/d。两组患者的疗程均为3个月。观察两组临床疗效,比较治疗前后两组肝功能指标[谷丙转氨酶（ALT）、总胆红素（TBiL）、白蛋白与球蛋白比值（A/G）]、谷草转氨酶（AST）与血小板（PLT）比值指数（APRI）评分、肝脏硬度测量（LSM）值、肝/脾CT值比值及血清肝纤维化指标[Ⅲ型前胶原N端肽（PC-Ⅲ）、透明质酸（HA）、Ⅳ型胶原（Ⅳ-C）、层黏连蛋白（LN）]和高迁移率族蛋白B1(HMGB1)、白细胞介素-17(IL-17)、可溶性P选择素（s Pselectin）水平。结果 治疗后,治疗组总有效率为90.36%,显著高于对照组的78.31%(P<0.05)。治疗后,两组血清ALT、TBiL水平均显...     

瑞舒伐他汀对慢性心力衰竭患者血清中细胞因子浓度的影响

目的：观察瑞舒伐他汀短期治疗对慢性心力衰竭（chronic heart failure,CHF）患者血清中可溶性选择素-E（sE-selectin）和P（sP-selectin）水平的影响。方法：70例CHF患者随机分为常规治疗组和干预组,常规治疗组给予强心、利尿、扩血管治疗,干预组在此基础上加用瑞舒伐他汀10mg/晚,治疗4周。采用ELISA测定其治疗前后血清中sE-selectin和sP-selectin水平。结果：心功能Ⅳ级患者血清sE-selectin和sP-selec-tin明显高于心功能Ⅱ、Ⅲ级患者（P〈0.01）,而心功能Ⅱ、Ⅲ级组间比较差异无统计学意义（P〉0.05）。两组治疗后血清sE-selectin和sP-selec-tin水平均较治疗前有显著下降（P〈0.05或P〈0.01）,干预组sE-selectin下降水平优于常规治疗组（P〈0.05）,而sP-selectin水平组间比较差异无统计学意义（P〉0.05）。结论：随着心功能恶化sE-selectin和sP-selectin水平明显升高,瑞舒伐他汀短期治疗能降低血清sE-selectin水平。     

Reduced platelet response to aspirin in patients with coronary artery disease and type 2 diabetes mellitus

Introduction

Diabetes mellitus is complicated by accelerated atherosclerosis, resulting in an increased risk of coronary artery disease (CAD) and thrombosis. Despite the proven benefits of aspirin, previous studies indicate a reduced cardiovascular protection from aspirin in diabetic patients. We aimed to investigate whether diabetes mellitus influenced the platelet response to aspirin in patients with CAD.

Materials and Methods

Platelet aggregation and activation were evaluated during aspirin treatment in 85 diabetic and 92 non-diabetic patients with CAD. Adherence to aspirin was carefully controlled. All patients had CAD verified by coronary angiography and were taking 75 mg non-enteric coated aspirin daily.

Results

Diabetic patients showed significantly higher levels of platelet aggregation compared to non-diabetic patients evaluated by VerifyNow^® Aspirin (p = 0.03) and Multiplate^® aggregometry using arachidonic acid (AA) 0.5 mM (p = 0.005) and 1.0 mM (p = 0.009). In addition, platelet activation determined by soluble P-selectin was significantly higher in diabetics compared to non-diabetics (p = 0.005). The higher AA-induced aggregation was associated with higher levels of HbA_1c. Compliance was confirmed by low levels of serum thromboxane B₂ (below 7.2 ng/mL). Diabetics had significantly higher levels of serum thromboxane B₂ (p < 0.0001).

Conclusions

Diabetic patients with CAD had significantly higher levels of both platelet aggregation and activation compared to non-diabetic patients with CAD despite treatment with the same dosage of aspirin. These findings may partly explain the reduced cardiovascular protection from aspirin in diabetic patients.     

尼莫地平联合艾地苯醌治疗脑梗死恢复期效果及对患者S-100β蛋白P选择素表达的影响

目的 探讨尼莫地平联合艾地苯醌治疗脑梗死恢复期效果及对患者S-100β蛋白、P选择素表达的影响.方法 将105例脑梗死恢复期患者按照简单随机化分组方法分为3组,各35例.对照A组给予尼莫地平治疗,对照B组给予艾地苯醌治疗,联合组给予尼莫地平联合艾地苯醌治疗,观察4周.采用美国国立卫生研究院卒中量表评定3组患者的临床疗效...     

早期抗栓治疗对急性非ST段抬高心肌梗死患者血浆可溶性P选择素的影响

目的:观察急性非ST段抬高心肌梗死患者血浆可溶性P(sP)选择素的动态变化及早期抗栓治疗对它的影响。方法:急性非ST段抬高心肌梗死发作6 h内入院患者18例,ELISA法测定心肌梗死发作6、12、24、48、72 h的sP选择素,并同时测定血常规和C反应蛋白浓度。所有患者在入院时进行阿司匹林加噻氯吡啶联用低分子肝素抗栓治疗,另选稳定性心绞痛患者21例及非冠心病患者20例作为对照。结果:(1)急性非ST段抬高心肌梗死患者发病6 h内sP选择素的浓度显著高于稳定性心绞痛组和非冠心病患者组(167．4±18．1 ng／ml vs 75．4±6．8 ng／ml vs 67．9±7．7 ng／ml,P<0．001);症状发作12 h、24 h sP选择素浓度仍维持在高水平(180．2±16．6 ng／ml,160．4±14．5 ng／ml);发病48 h、72 h．sP选择素浓度与对照纽比较无统计学差异。(2)急性非ST段抬高心肌梗死患者发病6 h白细胞计数,中性粒细胞百分比与C反应蛋白即升高,24 h达高峰,72 h后仍高于正常。结论:急性非ST段抬高心肌梗死发生6 h P选择素水平明显升高,早期抗栓治疗使P选择素在24 h后下降,第48小时达基线水平,但不能完全抑制炎症反应。     

Peripheral blood progenitor cell grafts contain high levels of platelet-secreted mediators

BACKGROUND: The cytokine network in peripheral blood progenitor cell (PBPC) grafts may affect hematopoietic reconstitution or the risk of postransplant relapse of malignant disorders through effects on normal progenitor cells or contaminating malignant cells. Whether thrombopoietin (TPO), SCF, and platelet-secreted mediators are parts of this network was investigated. STUDY DESIGN AND METHODS: Peripheral blood and PBPC plasma samples were collected consecutively from patients with malignant disorders who underwent PBPC harvest. Blood samples were collected immediately before and after apheresis. Patients underwent mobilization by chemotherapy plus G-CSF, except for one patient who received only G-CSF. Plasma levels were also determined for healthy controls. RESULTS: PBPC grafts had greater levels of platelet-secreted platelet factor 4 (PF4), beta-thromboglobulin, and platelet-derived growth factor isoform AB, as compared with venous levels in patients and controls. Although platelet and PF4 levels in autografts were significantly correlated, the graft:blood ratio was higher for PF4 than for platelets. In both the patients' blood and the autografts, TPO levels were increased from the levels in normal controls. Blood and graft levels of SCF were within the normal range. CONCLUSION: The cytokine network of PBPC autografts includes increased levels of TPO and several platelet-derived mediators.     

弥散性血管内凝血中敏感实验室检测指标的观察

目的：对反映弥散性血管内凝血（DIC）敏感的实验室检测指标进行比较分析，方法：检测120例Pre-DIC患者，30例DIC患者及30例健康志愿者血浆中PT，TT，KPTT，Fbg,D-Dimer,sP-选择素，凝血酶调节蛋白及BPC的变化，结果：Pre-DIC组及DIC患者组BPC，Fbg,D-Dimer,sP-选择素及TM与正常对照组比较有统计差异（P＜0．05或P＜0．01），对Pre-DIC组患者进行回顾性分析发现，对于诊断早期DIC的敏感实验室检测指标依次为；sP-选择素，TM、D－Dimer,BPC,Fbg,KTPP,TP,TT.敏感度依次为：96．7％，90．8％，81．7％，80．8％，76．6％，63．3％，56．7％，50．8％，而其特异性分别为60．5％，55．3％，73．6％，47．4％，26．3％，44．7％，42．1％，36．8％，虽然Pre-DIC组患者sP-选择素，TM较正常对照组有不同程度的升高，但其升高程度不及DIC组，随后发生DIC的76例患者sP-选择素，TM较非DIC患者升高明显，结论：sP-选择素，TM为DIC时出现异常最早的实验室指标之一。它们反映了患者体内高凝状态及内皮损伤程度，可作为诊断DIC得首选指标。