Combined effects of a high-fat diet and chronic valproic acid treatment on hepatic steatosis and hepatotoxicity in rats

Aim： To investigate the potential interactive effects of a high-fat diet （HFD） and valproic acid （VPA） on hepatic steatosis and hepatotoxicity in rats. Methods： Male SD rats were orally administered VPA （100 or 500 mg.kgl.d1） combined with HFD or a standard diet for 8 weeks. Blood and liver samples were analyzed to determine lipid levels and hepatic function biomarkers using commercial kit assays. Low- molecular-weight compounds in serum, urine and bile samples were analyzed using a metabonomic approach based on GC/TOF-MS. Results： HFD alone induced extensive hepatocyte steatosis and edema in rats, while VPA alone did not cause significant liver lesions. VPA significantly aggravated HFD-induced accumulation of liver lipids, and caused additional spotty or piecemeal necrosis, accompanied by moderate infiltration of inflammatory cells in the liver. Metabonomic analysis of serum, urine and bile samples revealed that HFD significantly increased the levells of amino acids, free fatty acids （FFAs） and 3-hydroxy-butanoic acid, whereas VPA markedly decreased the levels of amino acids, FFAs and the intermediate products of the tricarboxylic acid cycle （TCA） compared with the control group. HFD aggravated VPA-induced inhibition on lipid and amino acid metabolism. Conclusion： HFD magnifies VPA-induced impairment of mitochondria113-oxidation of FFAs and TCA, thereby increases hepatic steatosis and hepatotoxicity. The results suggest the patients receiving VPA treatment should be advised to avoid eating HFD.     

小体积良性前列腺增生治疗方式的选择

目的：寻找治疗小体积良性前列腺增生（ＢＰＨ）患者的最佳治疗方式。方法：对４２例小体积ＢＰＨ患者采用直肠Ｂ超及尿动力学检查相结合方法进行综合分析，如移行区指数（ＴＺＩ）〉０．４且存在膀胱颈梗阻者，采用ＴＵＲＰ治疗；如ＴＺＩ≤０．４且存在膀胱颈梗阻者，则行药物治疗，结果：ＴＺＩ〉０．４者ＴＵＲＰ治疗效果好（９３．３％），药物治疗效果较差（５０．０Ａ％０；ＴＺＩ≤０．４者药物治疗优于ＴＵＲＰ治疗：ＴＺＩ≤０．４而无膀胱颈梗阻者手术及药物治疗均无效。结论：治疗小体积ＢＰＨ患者的原则是尿动力学检查有膀胱颈梗阻存在，如ＴＺＩ〉）．４，宜行ＴＵＲＰ治疗；如ＴＺＩ〉０．４，则行药物治疗，如尿动力学检查无膀胱颈梗阻存在，则应继续寻找病因。