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Nilana MT Barros Raquel L Neves William N Addison Diego M Assis Monzur Murshed Adriana K Carmona Marc D McKee 《Journal of bone and mineral research》2013,28(3):688-699
X‐linked hypophosphatemia (XLH/HYP)—with renal phosphate wasting, hypophosphatemia, osteomalacia, and tooth abscesses—is caused by mutations in the zinc‐metallopeptidase PHEX gene (phosphate‐regulating gene with homologies to endopeptidase on the X chromosome). PHEX is highly expressed by mineralized tissue cells. Inactivating mutations in PHEX lead to distal renal effects (implying accumulation of a secreted, circulating phosphaturic factor) and accumulation in bone and teeth of mineralization‐inhibiting, acidic serine‐ and aspartate‐rich motif (ASARM)‐containing peptides, which are proteolytically derived from the mineral‐binding matrix proteins of the SIBLING family (small, integrin‐binding ligand N‐linked glycoproteins). Although the latter observation suggests a local, direct matrix effect for PHEX, its physiologically relevant substrate protein(s) have not been identified. Here, we investigated two SIBLING proteins containing the ASARM motif—osteopontin (OPN) and bone sialoprotein (BSP)—as potential substrates for PHEX. Using cleavage assays, gel electrophoresis, and mass spectrometry, we report that OPN is a full‐length protein substrate for PHEX. Degradation of OPN was essentially complete, including hydrolysis of the ASARM motif, resulting in only very small residual fragments. Western blotting of Hyp (the murine homolog of human XLH) mouse bone extracts having no PHEX activity clearly showed accumulation of an ~35 kDa OPN fragment that was not present in wild‐type mouse bone. Immunohistochemistry and immunogold labeling (electron microscopy) for OPN in Hyp bone likewise showed an accumulation of OPN and/or its fragments compared with normal wild‐type bone. Incubation of Hyp mouse bone extracts with PHEX resulted in the complete degradation of these fragments. In conclusion, these results identify full‐length OPN and its fragments as novel, physiologically relevant substrates for PHEX, suggesting that accumulation of mineralization‐inhibiting OPN fragments may contribute to the mineralization defect seen in the osteomalacic bone characteristic of XLH/HYP. © 2013 American Society for Bone and Mineral Research. 相似文献
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Dentin structure in familial hypophosphatemic rickets: benefits of vitamin D and phosphate treatment
Chaussain-Miller C Sinding C Septier D Wolikow M Goldberg M Garabedian M 《Oral diseases》2007,13(5):482-489
OBJECTIVE: To evaluate the outcome of 1-(OH) vitamin D and oral phosphate treatment on dentin structure in patients with familial hypophosphatemic rickets, and expression of SIBLINGs (a family of non-collagenous proteins involved in dentinogenesis) and osteocalcin. PATIENTS AND METHODS: Seven patients with familial hypophosphatemic rickets (age 3-16 years) were studied before or during treatment. Deciduous and permanent teeth were prepared for scanning electron microscopy (SEM) analysis and immunohistochemistry. RESULTS: Untreated or inadequately treated patients had necrotic teeth with impaired dentin mineralization including unmerged calcospherites and accumulation of non-collagenous proteins in wide interglobular spaces. Most of the primary incisors analyzed displayed fissures linking enamel subsurface to pulp horn. These elements may explain the bacterial penetration and dental abscesses despite the absence of carious lesions. Well-treated patients had healthy teeth with good dentin mineralization and little evidence of calcospherites. CONCLUSION: Treatment of hypophosphatemic children with 1-(OH) vitamin D and oral phosphate insures good dentin development and mineralization, and prevents clinical anomalies such as the dental necrosis classically associated with the disease. Starting treatment during early childhood and good adherence to the therapy are mandatory to observe these beneficial effects. 相似文献
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JieYuan Jin LiYang Zhang Shuai Guo Ke Tang Lei Zeng Rong Xiang JieYu Liang 《Journal of clinical laboratory analysis》2022,36(3)
BackgroundHypophosphatemia is mainly characterized by hypophosphatemia and a low level of 1alpha,25‐Dihydroxyvitamin D2 (1,25‐(OH)2D2) and/or 1alpha,25‐Dihydroxyvitamin D3 (1,25‐(OH)2D3) in the blood. Previous studies have demonstrated that variants in PHEX and FGF23 are primarily responsible for this disease. Although patients with variants of these two genes share almost the same symptoms, they exhibit the different hereditary pattern, X‐link dominant and autosome dominant, respectively. Three‐dimensional (3D) printing is a method which can accurately reconstruct physical objects, and its applications in orthopedics can contribute to realizing a more accurate surgical performance and a better outcome.MethodsAn X‐linked hypophosphatemia (XLH) family was recruited, with four patients across three generations. We screened candidate genes and filtered a duplication variant in PHEX. Variant analysis and co‐segregation confirmation were then performed. Before the operation of our patient, a digital model of our patient''s leg had been rebuilt upon the CT scan data, and a polylactic acid (PLA) model had been 3D‐printed.ResultsA novel duplication PHEX variant c.574dupG (p.A192GfsX20) was identified in a family with XLH. Its pathogenicity was confirmed by the co‐segregation assay and online bioinformatics database. The preoperative plan was made with the help of the PLA model. Then, arch osteotomy and transverse osteotomy were performed under the guidance of the previous simulation. The appearance of the surgical‐intervened leg was satisfactory.ConclusionsThis study identified a novel PHEX variant and showed that 3D printing tech is a very promising approach for corrective osteotomies. 相似文献
4.
Ito M Akai E Izuka M Segawa H Kuwahata M Miyamoto K 《Journal of bone and mineral metabolism》2004,22(1):3-11
Inactivating mutations and/or deletions of PHEX (Phosphate-regulating gene with Homologies to Endopeptidase on the X chromosome) are responsible for X-linked hypophosphatemic rickets in humans. In the present study, three Drosophila PHEX homologues (dPHEX-1, -2, -3) were isolated by the screening of a Drosophila cDNA library and expressed sequence tag (EST) database. The structural region involving motif II: 456WMXXXTKXXAXXK468 (numbered according to human PHEX), motif VI: 602WW603, and motif VIII: 746CXLW749 was conserved in the dPHEX family. Zinc-coordinating motifs (HEFTH and GENIADNGG) were also conserved in the dPHEX family. All three dPHEX genes were expressed during all stages of Drosophila development. The expression of dPHEX-1 was suppressed by dietary phosphate deprivation, but the expression of dPHEX-2 and that of dPHEX-3 were not affected. In-situ hybridization showed a ubiquitous distribution of dPHEX-1 and dPHEX-2, while dPHEX-3 was highly expressed in the larval brain. In an analysis of subcellular localization, dPHEX-1 was localized to intracellular organelles and dPHEX-3 was localized predominately in the plasma membrane of Drosophila embryonic S2 cells. Homozygosity of a dPHEX-1 mutation, a transposon insertion in the dPHEX-1 promoter region, was completely lethal at an early stage of embryonic development. The present study indicates that three homologues are likely involved in the phosphate homeostasis of Drosophila. 相似文献
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目的研究2例X-连锁低血磷性佝偻病(XLH)患儿及家系磷酸盐调节基因(PHEX)的突变类型,以明确其遗传学病因。方法回顾性分析2例XLH患者临床资料,应用高通量测序技术从基因组水平对先证者的XLH致病基因PHEX进行检测,并应用PCR-Sanger测序法对突变基因的家系分布进行验证。结果 2例患儿均检测到PHEX基因新发突变,1例为移码突变c.931dupC,导致翻译提前终止,产生截短蛋白p.Gln311Profs*13;另1例为剪接位点突变IVS14+1GA,导致外显子15跳跃,产生不完整的氨基酸链。2例患儿父母的基因表型均正常。结论 c.931dup C和IVS14+1GA是PHEX基因的两个新突变,可能是XLH新的致病性突变。 相似文献
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目的:研究PHEX抗体对小鼠牙胚发育的影响,探讨PHEX在牙齿发育过程的作用.方法:E17孕鼠尾静脉注射浓度分别为0.1mg/kg(A组)和0.5mg/kg(B组)的PHEX抗体.对照组N组注射0.1mol/LPBS.采用Mallory三色法染色观察钟状早期(E18)、钟状晚期(P1)、牙齿硬组织形成和矿化期(P3)牙胚的发育情况.免疫组织化学染色观察牙胚中PHEX蛋白的表达情况.结果:Mallory三色法染色结果显示;3组牙胚中釉质的发育无显著差异;成牙本质细胞的分化和牙本质的形成在A、N组中未见明显区别,而在B组中则明显延迟和抑制.E18、P1、P3 3组成釉细胞中的PHEX蛋白在牙胚中的表达均呈强阳性.P1、P3 A组和N组中成牙本质细胞中的PHEX表达也呈强阳性,但B组在P1期靠近基底膜的牙乳头细胞中表达呈弱阳性,在P3期也仅有少量的弱阳性表达.结论:0.5 mg/kg浓度的PHEX抗体可显著抑制成牙本质细胞的分化以及PHEX在其中的表达,进而抑制牙本质的生成;PHEX抗体对成釉细胞的发育和釉质的矿化无明显作用. 相似文献
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Bixia Zheng Chunli Wang Qiuxia Chen Ruochen Che Yugen Sha Fei Zhao Guixia Ding Wei Zhou Zhanjun Jia Songming Huang Ying Chen Aihua Zhang 《Journal of bone and mineral research》2020,35(9):1718-1725
X-linked hypophosphatemia (XLHR) is caused by loss-of-function mutations in the phosphate regulating endopeptidase homolog X-linked (PHEX) gene. Considerable controversy exists regarding genotype–phenotype correlations in XLHR. The present study describes the clinical features and molecular genetic bases of 53 pediatric patients with XLHR. Overall, 47 different mutations were identified, of which 27 were not previously described in the literature or entered in the Human Gene Mutation Database (HGMD). A high prevalence (72.34%) of truncating variants was observed in XLHR patients. The clinical presentation and severity of XLHR did not show an evident correlation between the truncating and non-truncating mutation types in our cohort. To further delineate the characteristics of PHEX variants underlying this nonsignificant trend, we assessed the effects of 10 PHEX variants on protein expression, cellular trafficking, and endopeptidase activity. Our results showed that the nonsense mutations p.Arg567*, p.Gln714*, and p.Arg747* caused a reduction of protein molecular weight and a trafficking defect. Among seven non-truncating mutations, the p.Cys77Tyr, p.Cys85Ser, p.Ile281Lys, p.Ile333del, p.Ala514Pro, and p.Gly572Ser mutants were not secreted into the medium and remained trapped inside cells in an immature form, whereas the p.Gly553Glu mutant was terminally glycosylated and secreted into the medium. We further assessed the endopeptidase activity of the p.Gly553Glu mutant using a quenched fluorogenic peptide substrate and revealed that the activity of p.Gly553Glu significantly reduced to 13% compared with the wild type, which indicated disruption of catalytic function. These data not only support the clinical results showing no correlation between disease severity and the type of PHEX mutation but also provide helpful molecular insights into the pathogenesis of XLHR. © 2020 American Society for Bone and Mineral Research. 相似文献
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Inherited hypophosphatemic disorders in children and the evolving mechanisms of phosphate regulation
Phosphorous is essential for multiple cellular functions and constitutes an important mineral in bone. Hypophosphatemia in
children leads to rickets resulting in abnormal growth and often skeletal deformities. Among various causes of low serum phosphorous
are inherited disorders associated with increased urinary excretion of phosphate, including autosomal dominant hypophosphatemic
rickets (ADHR), X-linked hypophosphatemia (XLH), autosomal recessive hypophosphatemia (ARHP), and hereditary hypophosphatemic
rickets with hypercalciuria (HHRH). Recent genetic analyses and subsequent biochemical and animal studies have revealed several
novel molecules that appear to play key roles in the regulation of renal phosphate handling. These include a protein with
abundant expression in bone, fibroblast growth factor 23 (FGF23), which has proven to be a circulating hormone that inhibits
tubular reabsorption of phosphate in the kidney. Two other bone-specific proteins, PHEX and dentin matrix protein 1 (DMP1),
appear to be necessary for limiting the expression of fibroblast growth factor 23, thereby allowing sufficient renal conservation
of phosphate. This review focuses on the clinical, biochemical, and genetic features of inherited hypophosphatemic disorders,
and presents the current understanding of hormonal and molecular mechanisms that govern phosphorous homeostasis. 相似文献