SHORT COMMUNICATION: GIFT and IUI in the district general hospital

Recent reports on gamete intra-Fallopian transfer (GIFT) inhospitals without IVF support have been encouraging. We reporton the use of GIFT and intrauterine insemination (IUI) at NorthamptonGeneral Hospital, where capital costs for setting up the programmehave been low. To date seven IUI and three GIFT treatments havebeen completed, resulting in three IUI positive pregnancy tests.One miscarried after several positive -HCGs and one GIFT pregnancywas achieved.     

Bilateral enzymic changes in rat nodose ganglia following unilateral cervical vagotomy.

Aspects of the metabolism of the nodose ganglia of the rat and their relationship to chromatolysis induced by unilateral cervical vagotomy were examined. The development of chromatolysis in the vagotomized ganglion followed a typical pattern. The changes in nucleic acid metabolism were elucidated by measurement of the activity of the pyrimidine biosynthesis specific enzyme: glutamine dependent carbamoyl phosphate synthetase. The activity of this enzyme showed a biphasic response supporting the conclusion that the first rapid increase in the rate of synthesis of ribosomal RNA may be a result of increased activity of the de novo pyrimidine biosynthetic pathway; a biphasic response also occurred in the enzyme activity in the contralateral ganglion. Lysosomal activity during the response was investigated by measuring the activity of acid phosphatase. There was also a bilateral change in the acid phosphatase activities of the two ganglia. This was more marked in the vagotomized ganglion, but followed a biphasic time response.The bilateral nature of the response observed in enzyme activities may reflect the similarities in metabolic requirements of axon regrowth in the vagotomized ganglion and axon collateral growth in the contralateral ganglion.     

中药部位复方DGH对STZ诱发的糖尿病大鼠早期视网膜形态学影响的实验研究

目的观察中药部位复方DGH对链脲佐菌素（STZ）诱发的糖尿病大鼠早期视网膜损害的形态学影响,探讨中医药治疗糖尿病视网膜病变的病理机制。方法采用STZ诱发的糖尿病大鼠模型,分为5组：空白对照组、递法明组和DGH低、中、高剂量组,另设有正常对照组。连续灌胃3个月处死大鼠,其中每只大鼠左眼做视网膜组织切片、右眼视网膜消化铺片。结果DGH各组血糖水平有不同程度的下降。空白对照组毛细血管基底膜边界模糊不清,可见毛细血管壁断裂、多处局灶性多形核白细胞阻塞管腔,偶见闭锁的毛细血管等;DGH高、中、低剂量组、递法明组对基底膜内皮细胞增殖,基底膜增厚等有不同程度的改善,其中以高剂量组显著。DGH低剂量组、递法明组大鼠视网膜毛细血管周细胞明显凋亡,可见毛细血管局部扩张、膨隆,微血管瘤形成及渗透性改变,DGH高、中剂量组毛细血管周细胞数目减少,但毛细血管管腔基本正常,血管走向平直。结论DGH能抑制视网膜周细胞丧失及基底膜增厚,对DM大鼠视网膜微血管形态学损害有一定的保护作用,并在一定程度上能调控STZ诱发的糖尿病大鼠的血糖水平。     

Population-level surgical outcomes for infantile hypertrophic pyloric stenosis

Objectives

Determine national outcomes for pyloromyotomy; how these are affected by: (i) surgical approach (open/laparoscopic), or (ii) centre type/volume and establish potential benchmarks of quality.